Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACIFYW_RS02350 Genome accession   NZ_CP173026
Coordinates   490245..491933 (-) Length   562 a.a.
NCBI ID   WP_404272710.1    Uniprot ID   -
Organism   Vibrio cholerae strain SPH 24-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 485245..496933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFYW_RS02325 (ACIFYW_02325) yacG 486311..486508 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  ACIFYW_RS02330 (ACIFYW_02330) zapD 486666..487406 (-) 741 WP_000207194.1 cell division protein ZapD -
  ACIFYW_RS02335 (ACIFYW_02335) coaE 487438..488046 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  ACIFYW_RS02340 (ACIFYW_02340) pilD 488043..488918 (-) 876 WP_267774830.1 A24 family peptidase Machinery gene
  ACIFYW_RS02345 (ACIFYW_02345) pilC 488976..490202 (-) 1227 WP_248431770.1 type II secretion system F family protein Machinery gene
  ACIFYW_RS02350 (ACIFYW_02350) pilB 490245..491933 (-) 1689 WP_404272710.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACIFYW_RS02355 (ACIFYW_02355) - 491940..492344 (-) 405 WP_057551631.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACIFYW_RS02360 (ACIFYW_02360) nadC 492589..493479 (-) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACIFYW_RS02365 (ACIFYW_02365) ampD 493777..494322 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACIFYW_RS02370 (ACIFYW_02370) - 494477..495865 (-) 1389 WP_001959226.1 IS4-like element ISVa18 family transposase -
  ACIFYW_RS02375 (ACIFYW_02375) fldB 496004..496522 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62050.12 Da        Isoelectric Point: 5.9043

>NTDB_id=1070030 ACIFYW_RS02350 WP_404272710.1 490245..491933(-) (pilB) [Vibrio cholerae strain SPH 24-01]
MLTNLVAILRQAELISATQEQAVITQVSASGTSVPEALLGLGIFHAQELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAT
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPDE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=1070030 ACIFYW_RS02350 WP_404272710.1 490245..491933(-) (pilB) [Vibrio cholerae strain SPH 24-01]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGATTACACAGGT
TAGCGCTTCGGGGACGTCGGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACGCCCAAGAGCTGACCGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCAACTTGTGCCAACAGCTCGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTTGCGGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAATTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCTGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGATCGATTCAAGGTGCCGCCAATCAGGGTAAAGAGATCAGCCAAGATGAGCTCGCCACT
CTGGTCAAGGTCAGTGATGACGAGCTGCAATCCATTGAAGATCTCAGCCAAGATGACTCGCCGGTTAGCCGTTTTATCAA
CCAGGTACTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCTTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCACGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGACGGGCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGCGTTTCCACGCTTCCCACCTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTAATGACAGGCCCAACCGGCAGCGGCAAAACCGTTTCACTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTAGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAACCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACT
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACGCTGCACACCAACTCGGCCGCCGAAACCGT
GATTCGCTTAGCCAATATGGGGGTGGAGCCGTTTAACCTTGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCATTTCAGCCCGATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGATATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAGCGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.11

100

0.991
  pilB Vibrio parahaemolyticus RIMD 2210633

74.021

100

0.74
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

51.81

93.416

0.484
  pilB Acinetobacter baylyi ADP1

50.484

91.993

0.464
  pilB Acinetobacter baumannii D1279779

50.289

92.349

0.464
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37.167

100

0.397
  pilF Thermus thermophilus HB27

40.111

96.263

0.386
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB Haemophilus influenzae Rd KW20

39.279

93.772

0.368

Multiple sequence alignment