Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACIFYW_RS02345 Genome accession   NZ_CP173026
Coordinates   488976..490202 (-) Length   408 a.a.
NCBI ID   WP_248431770.1    Uniprot ID   -
Organism   Vibrio cholerae strain SPH 24-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483976..495202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFYW_RS02320 (ACIFYW_02320) parC 483977..486262 (+) 2286 WP_000102515.1 DNA topoisomerase IV subunit A -
  ACIFYW_RS02325 (ACIFYW_02325) yacG 486311..486508 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  ACIFYW_RS02330 (ACIFYW_02330) zapD 486666..487406 (-) 741 WP_000207194.1 cell division protein ZapD -
  ACIFYW_RS02335 (ACIFYW_02335) coaE 487438..488046 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  ACIFYW_RS02340 (ACIFYW_02340) pilD 488043..488918 (-) 876 WP_267774830.1 A24 family peptidase Machinery gene
  ACIFYW_RS02345 (ACIFYW_02345) pilC 488976..490202 (-) 1227 WP_248431770.1 type II secretion system F family protein Machinery gene
  ACIFYW_RS02350 (ACIFYW_02350) pilB 490245..491933 (-) 1689 WP_404272710.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACIFYW_RS02355 (ACIFYW_02355) - 491940..492344 (-) 405 WP_057551631.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACIFYW_RS02360 (ACIFYW_02360) nadC 492589..493479 (-) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACIFYW_RS02365 (ACIFYW_02365) ampD 493777..494322 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45132.42 Da        Isoelectric Point: 10.3551

>NTDB_id=1070029 ACIFYW_RS02345 WP_248431770.1 488976..490202(-) (pilC) [Vibrio cholerae strain SPH 24-01]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKGKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLRTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1070029 ACIFYW_RS02345 WP_248431770.1 488976..490202(-) (pilC) [Vibrio cholerae strain SPH 24-01]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGTCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTCTCTTTATTGGCGCGCCTAACTCATCGCGTGAAAGGCAAAGACATCACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCCATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATTACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAAGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAACTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTTGTGTTGGTCGCGCTCGGGGTATC
TTACTTAATGCTCACCATGGTCATCCCAGAGTTTGAAAGCATGTTTAAAGGCTTTGGTGCTGAACTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTACAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCCTTCCAGATCCGTTTAAAAACCAGCCGCTTAGGGCTGAAATTTCCGATTATTGGCAA
TGTGCTCGCCAAAGCGTCCATCGCCAAATTCAGCCGCACGCTTGCCACCAGCTTTGCCGCGGGGATTCCGATCCTCGCCA
GTTTAAGAACCACCGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCCGCG
GGTATGCCGATGTACATTGCCATGCGCAATACCGAAGCCTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCAGGTCAATTGGATGACATGCTCAACAAGGTGGCCACCATCTATGAATTTGAAGTCGATAACACGGTTGATAACTTGG
GCAAAATTCTTGAGCCGCTGATCATCGTGTTTCTTGGGACGGTGGTGGGTGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.51

100

0.995
  pilC Vibrio campbellii strain DS40M4

73.75

98.039

0.723
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilC Legionella pneumophila strain ERS1305867

41.96

97.549

0.409
  pilG Neisseria gonorrhoeae MS11

41.294

98.529

0.407
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402

Multiple sequence alignment