Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACIFYW_RS01340 Genome accession   NZ_CP173026
Coordinates   285928..287643 (+) Length   571 a.a.
NCBI ID   WP_404272692.1    Uniprot ID   -
Organism   Vibrio cholerae strain SPH 24-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 280928..292643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFYW_RS01320 (ACIFYW_01320) pilM 283221..284237 (+) 1017 WP_404272691.1 type IV pilus assembly protein PilM Machinery gene
  ACIFYW_RS01325 (ACIFYW_01325) pilN 284227..284811 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  ACIFYW_RS01330 (ACIFYW_01330) pilO 284804..285394 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACIFYW_RS01335 (ACIFYW_01335) pilP 285384..285902 (+) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  ACIFYW_RS01340 (ACIFYW_01340) pilQ 285928..287643 (+) 1716 WP_404272692.1 type IV pilus secretin PilQ Machinery gene
  ACIFYW_RS01345 (ACIFYW_01345) aroK 287830..288354 (+) 525 WP_000818616.1 shikimate kinase AroK -
  ACIFYW_RS01350 (ACIFYW_01350) aroB 288382..289467 (+) 1086 WP_404272693.1 3-dehydroquinate synthase -
  ACIFYW_RS01355 (ACIFYW_01355) - 289522..290997 (+) 1476 WP_114716551.1 AAA family ATPase -
  ACIFYW_RS01360 (ACIFYW_01360) - 291067..291900 (+) 834 WP_000744680.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62351.47 Da        Isoelectric Point: 4.6094

>NTDB_id=1070026 ACIFYW_RS01340 WP_404272692.1 285928..287643(+) (pilQ) [Vibrio cholerae strain SPH 24-01]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPPAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVDSVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEEQGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1070026 ACIFYW_RS01340 WP_404272692.1 285928..287643(+) (pilQ) [Vibrio cholerae strain SPH 24-01]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCACCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTTGATGTGAAAGATTTTTCGACCCCTGTTGATAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCTGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTTATCTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAGCGGGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTTAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACCAACT
CGCTATTGATCCGAGAGCTGCCCGATAACATTGCCGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCAGGGGGTACGCTGGGGCGTAATGTCGAC
CAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTATTGGCGGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCGGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGTTTGAT
TACCACTAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTGAGCTTGAAGGTGACACCACAAATCACCCCCGATAACCGCTTGGTGCTCGACTTG
AGCGTGACCCAAGACCGACGTGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTATCGATCGATACTCAGCGTATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGCGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

99.475

100

0.995
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

99.475

100

0.995
  pilQ Vibrio campbellii strain DS40M4

78.182

96.322

0.753
  pilQ Legionella pneumophila strain ERS1305867

42.778

94.571

0.405
  pilQ Legionella pneumophila strain Lp02

42.778

94.571

0.405
  comQ Acinetobacter baylyi ADP1

39.336

100

0.394
  pilQ Acinetobacter baumannii strain A118

37.649

100

0.387
  pilQ Acinetobacter baumannii D1279779

37.649

100

0.387
  pilQ Pseudomonas aeruginosa PAK

39.162

96.147

0.377

Multiple sequence alignment