Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACIFYW_RS01320 Genome accession   NZ_CP173026
Coordinates   283221..284237 (+) Length   338 a.a.
NCBI ID   WP_404272691.1    Uniprot ID   -
Organism   Vibrio cholerae strain SPH 24-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 278221..289237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFYW_RS01305 (ACIFYW_01305) oxyR 279010..279903 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  ACIFYW_RS01310 (ACIFYW_01310) tnpA 280075..280512 (+) 438 WP_000503164.1 IS200/IS605-like element IS1004 family transposase -
  ACIFYW_RS01315 (ACIFYW_01315) - 280601..283078 (-) 2478 WP_162797021.1 penicillin-binding protein 1A -
  ACIFYW_RS01320 (ACIFYW_01320) pilM 283221..284237 (+) 1017 WP_404272691.1 type IV pilus assembly protein PilM Machinery gene
  ACIFYW_RS01325 (ACIFYW_01325) pilN 284227..284811 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  ACIFYW_RS01330 (ACIFYW_01330) pilO 284804..285394 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACIFYW_RS01335 (ACIFYW_01335) pilP 285384..285902 (+) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  ACIFYW_RS01340 (ACIFYW_01340) pilQ 285928..287643 (+) 1716 WP_404272692.1 type IV pilus secretin PilQ Machinery gene
  ACIFYW_RS01345 (ACIFYW_01345) aroK 287830..288354 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37770.45 Da        Isoelectric Point: 7.3328

>NTDB_id=1070022 ACIFYW_RS01320 WP_404272691.1 283221..284237(+) (pilM) [Vibrio cholerae strain SPH 24-01]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRWHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRSLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1070022 ACIFYW_RS01320 WP_404272691.1 283221..284237(+) (pilM) [Vibrio cholerae strain SPH 24-01]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCGCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTGGCATGCCGTTAAAAAAGCGGGTTTTGAACCC
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAATAATGTGGCAAAACGCAAACGCTCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.408

100

0.994
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment