Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACAM43_RS01950 Genome accession   NZ_AP028671
Coordinates   421497..422726 (-) Length   409 a.a.
NCBI ID   WP_369631666.1    Uniprot ID   -
Organism   Variovorax sp. V77     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416497..427726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM43_RS01925 (GmRootV77_03790) zapD 416510..417265 (-) 756 WP_070063618.1 cell division protein ZapD -
  ACAM43_RS01930 (GmRootV77_03800) - 417302..418333 (-) 1032 WP_369631663.1 hypothetical protein -
  ACAM43_RS01935 (GmRootV77_03810) - 418340..419833 (-) 1494 WP_095743910.1 MBOAT family protein -
  ACAM43_RS01940 (GmRootV77_03820) coaE 419910..420518 (-) 609 WP_369631664.1 dephospho-CoA kinase -
  ACAM43_RS01945 (GmRootV77_03830) - 420532..421497 (-) 966 WP_369631665.1 A24 family peptidase -
  ACAM43_RS01950 (GmRootV77_03840) pilC 421497..422726 (-) 1230 WP_369631666.1 type II secretion system F family protein Machinery gene
  ACAM43_RS01955 (GmRootV77_03850) pilB 422753..424486 (-) 1734 WP_369631667.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM43_RS01965 (GmRootV77_03860) - 424731..425723 (-) 993 WP_070063612.1 polyprenyl synthetase family protein -
  ACAM43_RS01970 (GmRootV77_03870) rplU 425906..426217 (+) 312 WP_042578903.1 50S ribosomal protein L21 -
  ACAM43_RS01975 (GmRootV77_03880) rpmA 426230..426490 (+) 261 WP_013543198.1 50S ribosomal protein L27 -
  ACAM43_RS01980 (GmRootV77_03890) cgtA 426563..427639 (+) 1077 WP_062474512.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44710.42 Da        Isoelectric Point: 9.6713

>NTDB_id=106837 ACAM43_RS01950 WP_369631666.1 421497..422726(-) (pilC) [Variovorax sp. V77]
MATVASSRSQVTHKEFVFEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKAIKPKDIAIFTRQLA
TMMKAGVPLLQSFDIVGRGNANASVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEDLLDRLATY
MEKTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKQVFSSFGADLPAPTLFVMAMSEFFVSYWWLIFGVLGGGI
YFFLQAWKRNERVQKVMDRLLLRIPIFGTLIEKSCVARWTRTLATMFAAGVPLVEALDSVGGASGNSVYGDATAKIQQEV
STGTSLTTAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=106837 ACAM43_RS01950 WP_369631666.1 421497..422726(-) (pilC) [Variovorax sp. V77]
ATGGCAACAGTGGCATCCTCCCGCTCCCAGGTCACTCACAAGGAATTCGTCTTCGAATGGGAAGGCAAGGACCGCAACGG
CAAGCTGGTGCGCGGCGAACTTCGCGCCGCCGGGGAGAACCAGGTGCAGGCGGCCTTGCGTCGCCAGGGCGTGCTGGCCT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGCAAGGCGATCAAGCCCAAGGACATCGCGATCTTCACGCGCCAGCTCGCG
ACCATGATGAAGGCCGGGGTGCCGCTGCTGCAGTCCTTCGACATCGTGGGTCGCGGCAACGCGAACGCGAGTGTCGCCAA
GCTGCTGAACGACATCCGCAGCGATGTCGAGACCGGCACCTCGCTGTCGGCCGCTTTTCGCAAGTTTCCGAAGTACTTCG
ACAACCTCTATTGCAACCTGGTGGAAGCCGGTGAGGCGGCCGGTATTTTGGAGGACCTGCTCGACCGCTTGGCGACCTAC
ATGGAAAAGACCGAAGCGATCAAGTCCAAGATCAAGTCGGCATTGATGTACCCGACCTCGGTGGTCGTCGTGGCCTTCGT
GGTGGTGGCGATCATCATGATCTTCGTGATCCCTGCCTTCAAGCAGGTGTTCAGTTCGTTCGGTGCTGACCTGCCCGCGC
CTACGCTCTTCGTGATGGCAATGAGCGAGTTCTTCGTTTCCTATTGGTGGCTGATCTTCGGCGTGCTCGGTGGTGGCATC
TATTTCTTTCTGCAAGCGTGGAAACGCAATGAGCGCGTCCAGAAGGTCATGGACCGCCTGCTGCTGCGCATTCCCATTTT
CGGCACGCTGATCGAGAAATCCTGCGTGGCCCGCTGGACCCGCACGCTCGCCACCATGTTCGCGGCCGGCGTGCCGCTGG
TGGAGGCGCTCGACTCGGTGGGCGGCGCATCGGGCAACTCGGTGTACGGCGACGCCACCGCCAAGATCCAGCAGGAGGTC
TCGACCGGCACCAGCCTCACGACGGCCATGACCAACGTCAACCTGTTCCCCTCGATGGTGATTCAAATGACCGCCATCGG
CGAGGAATCAGGCTCGATCGACCACATGCTCGGCAAGGCCGCCGACTTCTACGAGTCCGAAGTCGACGACATGGTTGCCG
GCCTCTCCAGCCTGATGGAACCCATCATCATCGTGTTCCTGGGCGTCATCATCGGCGGCATCGTGGTGTCGATGTACCTG
CCCATCTTCAAGCTCGGCCAGGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.465

98.778

0.528
  pilG Neisseria gonorrhoeae MS11

52.645

97.066

0.511
  pilG Neisseria meningitidis 44/76-A

52.393

97.066

0.509
  pilC Acinetobacter baylyi ADP1

50.608

100

0.509
  pilC Legionella pneumophila strain ERS1305867

49.749

97.311

0.484
  pilC Acinetobacter baumannii D1279779

49.874

97.066

0.484
  pilC Vibrio cholerae strain A1552

41.206

97.311

0.401
  pilC Thermus thermophilus HB27

38.425

100

0.394
  pilC Vibrio campbellii strain DS40M4

39.055

98.289

0.384

Multiple sequence alignment