Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAM43_RS01955 Genome accession   NZ_AP028671
Coordinates   422753..424486 (-) Length   577 a.a.
NCBI ID   WP_369631667.1    Uniprot ID   -
Organism   Variovorax sp. V77     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417753..429486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM43_RS01935 (GmRootV77_03810) - 418340..419833 (-) 1494 WP_095743910.1 MBOAT family protein -
  ACAM43_RS01940 (GmRootV77_03820) coaE 419910..420518 (-) 609 WP_369631664.1 dephospho-CoA kinase -
  ACAM43_RS01945 (GmRootV77_03830) - 420532..421497 (-) 966 WP_369631665.1 A24 family peptidase -
  ACAM43_RS01950 (GmRootV77_03840) pilC 421497..422726 (-) 1230 WP_369631666.1 type II secretion system F family protein Machinery gene
  ACAM43_RS01955 (GmRootV77_03850) pilB 422753..424486 (-) 1734 WP_369631667.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM43_RS01965 (GmRootV77_03860) - 424731..425723 (-) 993 WP_070063612.1 polyprenyl synthetase family protein -
  ACAM43_RS01970 (GmRootV77_03870) rplU 425906..426217 (+) 312 WP_042578903.1 50S ribosomal protein L21 -
  ACAM43_RS01975 (GmRootV77_03880) rpmA 426230..426490 (+) 261 WP_013543198.1 50S ribosomal protein L27 -
  ACAM43_RS01980 (GmRootV77_03890) cgtA 426563..427639 (+) 1077 WP_062474512.1 Obg family GTPase CgtA -
  ACAM43_RS01985 (GmRootV77_03900) proB 427720..428859 (+) 1140 WP_062474510.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62554.84 Da        Isoelectric Point: 5.8798

>NTDB_id=106838 ACAM43_RS01955 WP_369631667.1 422753..424486(-) (pilB) [Variovorax sp. V77]
MAAAEIPVKETTQIALPGLARALVSAGKLAAKTAEDIYQKSLSGRTSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAASAAETI
NSIVGTEFEFDDVTADTSGDANEQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHHYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARMGIDALGYDADEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCMLCRAPIDIPR
EALLDAGFKDEDINGTWKPYRPVGCAACSGGYKGRVGIYQVMPISEAIQEIILRDGSALDIANQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNL

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=106838 ACAM43_RS01955 WP_369631667.1 422753..424486(-) (pilB) [Variovorax sp. V77]
ATGGCCGCTGCCGAAATTCCCGTTAAAGAAACTACGCAGATCGCCCTTCCCGGCTTGGCGCGCGCCCTCGTGTCGGCCGG
CAAGCTCGCGGCGAAGACAGCCGAAGACATCTATCAGAAGTCGCTGAGCGGCCGCACCAGCTTCATCGCCGAACTGACCG
GCACCGGCGCCGTTTCGGCTGCGGACCTCGCCCACACCCTCTCAAGCGCCTTCGGCGCCCCGCTGCTCGACCTCGATGCC
ATCGATCACCAGCGCCTGCCCAAGGACCTGCTCGATCCGAAGCTCTGCCATGCGTACCGCATCGTCGTTCTCAGCAAGCG
CAACAACCGCCTGATCGTCGCGACGGCCGACCCGTCGGACCAGCAGGCCGCGGAAAAAATCAAGTTCGCGTCGCAGATGG
GCGTGGACTGGGTCATCGCGGAGTACGACAAGCTCTCGCGCATGATCGAAGCCGCCGCTGCGAGCGCTGCGGAAACGATC
AACAGCATCGTCGGCACCGAGTTCGAGTTCGACGACGTCACGGCCGACACCTCGGGCGACGCCAACGAACAGGCCATCGC
CGAGGTCGAAGACGCACCTGTGGTGCGCTTCCTGCACAAGATGCTGCTCGACGCCGTGAGCATGCGAGCCTCGGACATCC
ACTTCGAGCCCTACGAACACCACTACCGGGTGCGCTTTCGAGTCGACGGCGAGCTGCGCGAAATCGCCAGCCCACCCACG
GTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATTTCGCGGCTCGACATCTCGGAAAAACGCGTGCCGCAAGACGG
CCGCATGAAGCTCAAGATCGGGCCCGATCGCGTGATCGACTTTCGCGTGAGCACGTTGCCCACGCTGTTCGGCGAAAAGA
TCGTCGTGCGTATCCTGGACCCGAGCAGCGCGCGCATGGGCATCGACGCCCTGGGCTACGACGCCGACGAAAAAGAGCGA
CTCCTCCACGCCATCGGCCGCCCCTATGGCATGGTGCTGGTGACCGGCCCCACGGGTTCGGGCAAGACCGTGTCGCTCTA
CACCTGCCTGAACCTGCTCAACCAGCCCGGCGTCAACATCGCAACGGCGGAAGACCCGTCGGAAATCAACCTGCCCGGCG
TCAACCAGGTCAACGTCAACGAGAAGGCCGGCCTCACTTTCGCGGCCGCGCTGCGCGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAAACGGGCCACCTGGTGCT
GTCGACGCTGCACACCAACGACGCCCCGACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCGTTCAATATCGCGT
CGAGCGTGATCCTGATCACCGCGCAACGCCTCGCGCGCCGCCTGTGCATGCTGTGCCGCGCACCGATCGACATCCCGCGC
GAAGCCCTGCTCGATGCCGGCTTCAAAGACGAAGACATCAACGGCACCTGGAAGCCCTACCGCCCGGTCGGCTGCGCCGC
CTGCAGCGGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATTTCGGAAGCCATCCAGGAAATCATCCTGC
GCGACGGCAGTGCGCTAGACATCGCGAACCAATCCGAAGCCGAAGGCGTGCGCTCGCTGCGCCAGTCCGGCCTTCGCAAA
GTCATGCAGGGCCTCACTTCACTCGAAGAAGTCGTCGCCTGCACCAACCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-157)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.31

99.48

0.53
  pilB Acinetobacter baumannii D1279779

53.929

97.054

0.523
  pilF Neisseria gonorrhoeae MS11

52.847

97.4

0.515
  pilB Legionella pneumophila strain ERS1305867

48.319

97.92

0.473
  pilB Vibrio cholerae strain A1552

46.607

97.054

0.452
  pilB Vibrio campbellii strain DS40M4

45.826

97.574

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.276

97.227

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.147

94.107

0.378
  pilF Thermus thermophilus HB27

37.61

98.614

0.371

Multiple sequence alignment