Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACF0KO_RS19945 Genome accession   NZ_CP172307
Coordinates   4547240..4548457 (-) Length   405 a.a.
NCBI ID   WP_064030934.1    Uniprot ID   A0A177N9M5
Organism   Methylomonas sp. 2B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4542240..4553457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0KO_RS19910 (ACF0KO_19910) - 4542317..4542541 (+) 225 WP_064030927.1 sulfurtransferase TusA family protein -
  ACF0KO_RS19915 (ACF0KO_19915) argJ 4542587..4543801 (+) 1215 WP_064030928.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  ACF0KO_RS19920 (ACF0KO_19920) - 4543803..4544759 (+) 957 WP_064030929.1 Nudix family hydrolase -
  ACF0KO_RS19925 (ACF0KO_19925) yacG 4544744..4544947 (-) 204 WP_064030930.1 DNA gyrase inhibitor YacG -
  ACF0KO_RS19930 (ACF0KO_19930) zapD 4544947..4545717 (-) 771 WP_082885650.1 cell division protein ZapD -
  ACF0KO_RS19935 (ACF0KO_19935) coaE 4545714..4546325 (-) 612 WP_064030932.1 dephospho-CoA kinase -
  ACF0KO_RS19940 (ACF0KO_19940) pilD 4546325..4547191 (-) 867 WP_064030933.1 A24 family peptidase Machinery gene
  ACF0KO_RS19945 (ACF0KO_19945) pilC 4547240..4548457 (-) 1218 WP_064030934.1 type II secretion system F family protein Machinery gene
  ACF0KO_RS19950 (ACF0KO_19950) pilB 4548460..4550175 (-) 1716 WP_064030935.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF0KO_RS19955 (ACF0KO_19955) thiC 4550323..4552191 (-) 1869 WP_064030936.1 phosphomethylpyrimidine synthase ThiC -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44273.24 Da        Isoelectric Point: 6.5109

>NTDB_id=1066205 ACF0KO_RS19945 WP_064030934.1 4547240..4548457(-) (pilC) [Methylomonas sp. 2B]
MAKQDEQIEFNWDGFDKQNKKTKGTIMAKSEVIARTELRRMGIRVVGIKRKAKPLFGAKVQKITSADIAIFARQLATMLE
AGVPLVQSFDIIGRGHDNPSMAELLLAIKADIEGGDTMAQALAKRPIYFDDLFCNLVAAGEHAGVLETLLDKIATYKEKT
ESIKKKVKKALTYPVAVLVVAFIVTAILLIFVVPVFEDLFKSFGADLPAFTQFVIGLSAWVQEWWWAVLLVIGGGGYTFV
YFKKRSQAFNHFLDRTLLKIPVVGMILEKSAIARFARTLSTMSAAGVPLVEALVSVAGACGNILFHDAVMSVRDEVSTGQ
QLKVALEQTGMFPNMVVQMVAIGEESGSIDAMLDKVADFFEEEVDNLVDNLSSLMEPIIMAILGVLVGGLIIAMYLPIFK
LGAAM

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1066205 ACF0KO_RS19945 WP_064030934.1 4547240..4548457(-) (pilC) [Methylomonas sp. 2B]
ATGGCAAAGCAAGACGAGCAAATCGAGTTCAATTGGGACGGTTTCGATAAGCAAAACAAAAAAACCAAGGGTACGATCAT
GGCCAAAAGCGAAGTGATTGCCCGAACCGAGCTGCGGCGGATGGGGATACGCGTCGTCGGCATCAAGCGCAAGGCTAAGC
CGCTATTCGGCGCCAAGGTGCAGAAAATCACCAGCGCGGATATCGCGATATTTGCCAGACAGTTGGCGACGATGTTGGAA
GCCGGCGTGCCGCTGGTGCAGTCCTTCGACATTATCGGCCGCGGCCACGACAATCCCAGCATGGCCGAGCTGTTGCTGGC
GATCAAGGCCGACATCGAAGGCGGCGACACCATGGCCCAGGCCTTGGCGAAACGGCCTATCTATTTCGACGATTTGTTCT
GCAATTTGGTCGCCGCGGGCGAGCATGCCGGGGTGTTGGAAACCTTGCTGGACAAGATTGCCACCTACAAGGAAAAAACC
GAGTCGATCAAGAAAAAGGTCAAAAAGGCACTGACCTACCCGGTCGCGGTGTTGGTGGTTGCCTTCATCGTGACCGCGAT
TTTGCTGATTTTCGTGGTGCCGGTGTTCGAGGACTTGTTTAAAAGTTTCGGCGCCGACCTGCCGGCCTTCACTCAATTCG
TGATCGGCTTGTCTGCCTGGGTACAGGAATGGTGGTGGGCGGTGTTGCTGGTGATCGGCGGCGGCGGTTATACCTTTGTC
TACTTCAAGAAACGCTCGCAGGCGTTTAACCATTTTCTCGATAGAACGCTGTTGAAGATTCCGGTGGTCGGCATGATTTT
GGAAAAATCGGCGATCGCCCGTTTTGCTCGCACGCTATCGACGATGTCGGCGGCCGGCGTGCCGCTGGTCGAGGCCTTGG
TGTCGGTGGCCGGCGCTTGCGGCAATATTCTGTTTCATGATGCGGTAATGAGCGTCCGCGACGAAGTATCCACCGGTCAG
CAGTTGAAGGTGGCGTTGGAACAAACCGGCATGTTTCCGAATATGGTGGTGCAAATGGTCGCCATCGGCGAGGAATCCGG
CTCTATCGATGCGATGTTGGATAAGGTGGCGGATTTCTTCGAAGAAGAGGTAGACAACCTGGTCGACAACCTCAGCAGCC
TGATGGAGCCGATCATCATGGCCATCTTGGGCGTATTGGTCGGCGGCTTGATCATCGCGATGTATTTACCCATCTTCAAG
CTCGGCGCCGCGATGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A177N9M5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

59.296

98.272

0.583
  pilC Pseudomonas stutzeri DSM 10701

57.36

97.284

0.558
  pilC Acinetobacter baumannii D1279779

54.43

97.531

0.531
  pilC Acinetobacter baylyi ADP1

52.709

100

0.528
  pilG Neisseria gonorrhoeae MS11

46.269

99.259

0.459
  pilG Neisseria meningitidis 44/76-A

47.532

95.062

0.452
  pilC Vibrio cholerae strain A1552

40.759

97.531

0.398
  pilC Vibrio campbellii strain DS40M4

40.201

98.272

0.395
  pilC Thermus thermophilus HB27

37.186

98.272

0.365

Multiple sequence alignment