Detailed information NTG1066204

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACF0KO_RS19940
• Genome accession: NZ_CP172307
• Coordinates: 4546325..4547191 (-)
• Length: 288 a.a.
• NCBI ID: WP_064030933.1
• Uniprot ID: A0A177N9U3
• Organism: Methylomonas sp. 2B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4541325..4552191

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF0KO_RS19910 (ACF0KO_19910)	-	4542317..4542541 (+)	225	WP_064030927.1	sulfurtransferase TusA family protein	-
ACF0KO_RS19915 (ACF0KO_19915)	argJ	4542587..4543801 (+)	1215	WP_064030928.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
ACF0KO_RS19920 (ACF0KO_19920)	-	4543803..4544759 (+)	957	WP_064030929.1	Nudix family hydrolase	-
ACF0KO_RS19925 (ACF0KO_19925)	yacG	4544744..4544947 (-)	204	WP_064030930.1	DNA gyrase inhibitor YacG	-
ACF0KO_RS19930 (ACF0KO_19930)	zapD	4544947..4545717 (-)	771	WP_082885650.1	cell division protein ZapD	-
ACF0KO_RS19935 (ACF0KO_19935)	coaE	4545714..4546325 (-)	612	WP_064030932.1	dephospho-CoA kinase	-
ACF0KO_RS19940 (ACF0KO_19940)	pilD	4546325..4547191 (-)	867	WP_064030933.1	A24 family peptidase	Machinery gene
ACF0KO_RS19945 (ACF0KO_19945)	pilC	4547240..4548457 (-)	1218	WP_064030934.1	type II secretion system F family protein	Machinery gene
ACF0KO_RS19950 (ACF0KO_19950)	pilB	4548460..4550175 (-)	1716	WP_064030935.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACF0KO_RS19955 (ACF0KO_19955)	thiC	4550323..4552191 (-)	1869	WP_064030936.1	phosphomethylpyrimidine synthase ThiC	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31913.90 Da
• Isoelectric Point: 6.8271

>NTDB_id=1066204 ACF0KO_RS19940 WP_064030933.1 4546325..4547191(-) (pilD) [Methylomonas sp. 2B]
MLTMLEQFPPLLIGVALLLGLLVGSFLNVVIYRLPVMLQRDWRRDCHEFLQLPYSEPTEAFNLMWPASHCPACNAPIKAY
QNIPVLSYLLLAGRCANCATPIAWRYPLVEAFTGLCSALVAWKLGFGLGLAFGLLLTWSLIALSFIDFDHQLLPDSITLP
MLWLGLLLSLFGVYAEPRDAIIGAAAGYWVLWSVYQGFKLLTGKEGMGFGDFKLLALLGAWFGWQYLPQIVLLSSLLGAL
VGVTMIATGRREASKPIPFGPYLAAAGWIAMLWGDDINRWYLHFAGLN

Nucleotide

• Length: 867 bp

>NTDB_id=1066204 ACF0KO_RS19940 WP_064030933.1 4546325..4547191(-) (pilD) [Methylomonas sp. 2B]
ATGCTGACGATGTTGGAACAATTTCCGCCGCTGCTGATTGGCGTGGCGCTACTGCTAGGTCTACTGGTCGGCAGTTTCTT
GAATGTGGTGATTTACCGTTTGCCGGTGATGCTGCAACGCGATTGGCGGCGCGATTGCCACGAGTTTTTACAACTGCCGT
ATTCGGAGCCCACCGAGGCTTTCAATCTGATGTGGCCGGCTTCGCATTGTCCGGCCTGTAACGCGCCGATCAAGGCCTAT
CAGAACATTCCGGTGTTGAGCTATTTACTGTTGGCTGGCCGGTGCGCGAACTGCGCTACCCCTATCGCTTGGCGCTATCC
ATTGGTCGAGGCGTTTACCGGACTGTGTTCCGCGCTGGTGGCCTGGAAGTTGGGCTTCGGGCTGGGGTTGGCATTCGGTT
TGTTGTTGACCTGGAGCTTGATTGCGTTGAGTTTCATTGATTTCGACCATCAATTGTTGCCCGACAGCATCACTTTGCCG
ATGTTGTGGCTGGGTTTGTTGCTGAGTTTGTTCGGTGTCTATGCCGAACCGCGCGATGCGATCATCGGCGCGGCGGCTGG
CTATTGGGTACTGTGGAGCGTTTACCAGGGCTTCAAACTGCTGACCGGCAAGGAAGGGATGGGCTTCGGCGATTTCAAGT
TATTGGCCCTACTTGGCGCCTGGTTCGGTTGGCAGTATCTGCCGCAGATTGTGTTGCTGTCGTCGCTGTTGGGCGCGTTG
GTCGGCGTGACGATGATCGCGACCGGGCGGCGCGAAGCGTCCAAGCCGATTCCGTTCGGGCCTTATCTGGCGGCGGCCGG
TTGGATCGCCATGCTGTGGGGCGACGACATCAACCGCTGGTATCTGCATTTTGCCGGATTGAATTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-123)

3D structure

• AlphaFold DB: A0A177N9U3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	52.5	97.222	0.51
pilD	Acinetobacter nosocomialis M2	52.143	97.222	0.507
pilD	Vibrio cholerae strain A1552	51.449	95.833	0.493
pilD	Neisseria gonorrhoeae MS11	48.958	100	0.49
pilD	Vibrio campbellii strain DS40M4	49.47	98.264	0.486