Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACF0KO_RS19950 Genome accession   NZ_CP172307
Coordinates   4548460..4550175 (-) Length   571 a.a.
NCBI ID   WP_064030935.1    Uniprot ID   -
Organism   Methylomonas sp. 2B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4543460..4555175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0KO_RS19920 (ACF0KO_19920) - 4543803..4544759 (+) 957 WP_064030929.1 Nudix family hydrolase -
  ACF0KO_RS19925 (ACF0KO_19925) yacG 4544744..4544947 (-) 204 WP_064030930.1 DNA gyrase inhibitor YacG -
  ACF0KO_RS19930 (ACF0KO_19930) zapD 4544947..4545717 (-) 771 WP_082885650.1 cell division protein ZapD -
  ACF0KO_RS19935 (ACF0KO_19935) coaE 4545714..4546325 (-) 612 WP_064030932.1 dephospho-CoA kinase -
  ACF0KO_RS19940 (ACF0KO_19940) pilD 4546325..4547191 (-) 867 WP_064030933.1 A24 family peptidase Machinery gene
  ACF0KO_RS19945 (ACF0KO_19945) pilC 4547240..4548457 (-) 1218 WP_064030934.1 type II secretion system F family protein Machinery gene
  ACF0KO_RS19950 (ACF0KO_19950) pilB 4548460..4550175 (-) 1716 WP_064030935.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF0KO_RS19955 (ACF0KO_19955) thiC 4550323..4552191 (-) 1869 WP_064030936.1 phosphomethylpyrimidine synthase ThiC -
  ACF0KO_RS19960 (ACF0KO_19960) recD 4552469..4554103 (+) 1635 WP_064030937.1 exodeoxyribonuclease V subunit alpha -
  ACF0KO_RS19965 (ACF0KO_19965) - 4554100..4554519 (+) 420 WP_064030938.1 hypothetical protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63116.75 Da        Isoelectric Point: 6.2556

>NTDB_id=1066206 ACF0KO_RS19950 WP_064030935.1 4548460..4550175(-) (pilB) [Methylomonas sp. 2B]
MATAPSNIHFSGLAKCLIQKGLLTEETAAAYLQEAQKRKQAFVSYLVEHKVLDSRTVASIASAEFGVPFFDLDAIDVASL
PIKMVSEKLIRQHHALPLFKRGNRLFVATADPTNFQALDEIKFHTRHNTENILVEEDKLMKMIDTALEAADTSMKDLLDD
DLDNIDISAGDEAKPAEEVGGSDIDDAPIVRFVNKILLDSIKRGVSDIHMEPFEKKFRIRFRADGILSEVASPPVGIANR
IISRIKVMSKMDIAERRVPQDGRIKMQISKNRAIDFRVNTCPTLFGEKVVMRILDPTSAQIGIEKLGFEPVQQKNFLDAI
HRPYGMVLVTGPTGSGKTVSLYTGLNILNSVERNISTAEDPVEITVEGINQVNVNAKAGLTFAEALRAFLRQDPDIIMVG
EIRDLETASIAVKAAQTGHLVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVNLIMAQRLARRLCEHCKKEADFPENIWLS
AGFSKEEIGTFKIYEPVGCEHCNNGYKGRVGIYQVMPISEAMRALILRGGNAMDMAALSKTEGINDLRASGLLKIRQGIT
SLEEIDRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1066206 ACF0KO_RS19950 WP_064030935.1 4548460..4550175(-) (pilB) [Methylomonas sp. 2B]
ATGGCGACAGCTCCTTCCAATATTCATTTCAGCGGCTTGGCCAAGTGCCTGATCCAAAAAGGCCTGTTGACTGAAGAGAC
TGCCGCCGCCTATCTGCAAGAAGCCCAAAAACGCAAGCAAGCCTTCGTCAGTTATCTGGTCGAGCATAAAGTGCTCGACA
GTCGGACCGTGGCCTCCATCGCTTCCGCCGAGTTCGGCGTGCCGTTTTTCGATTTGGATGCGATAGATGTGGCGTCGTTG
CCTATCAAAATGGTCAGCGAAAAGCTGATCCGCCAACATCACGCTCTGCCACTGTTCAAACGCGGTAACCGCTTGTTCGT
CGCCACCGCCGATCCGACCAATTTCCAGGCGCTGGACGAGATCAAGTTCCATACTCGGCACAATACCGAGAACATTCTGG
TCGAAGAAGACAAATTGATGAAGATGATCGATACCGCGCTGGAAGCGGCCGATACATCAATGAAGGATTTGCTCGACGAC
GACCTGGACAACATCGATATCTCCGCCGGCGACGAAGCCAAGCCGGCCGAGGAAGTCGGCGGTTCGGACATCGACGATGC
GCCCATCGTCCGTTTCGTCAACAAAATTCTGCTCGATTCGATCAAGCGCGGCGTTTCGGATATTCACATGGAGCCGTTCG
AGAAGAAGTTCCGGATCCGGTTTCGGGCCGACGGCATTTTGTCCGAAGTCGCCAGTCCGCCGGTCGGTATTGCCAATCGG
ATCATATCCCGGATCAAGGTCATGTCGAAAATGGACATCGCCGAGCGGCGGGTGCCCCAGGACGGCCGGATCAAAATGCA
AATTTCCAAGAATCGGGCCATCGATTTCCGGGTGAATACCTGTCCGACGCTGTTCGGCGAAAAAGTGGTGATGCGGATAC
TCGATCCCACCAGCGCCCAGATCGGTATCGAAAAATTGGGTTTCGAGCCTGTTCAACAAAAAAACTTTTTGGACGCCATT
CACCGGCCCTACGGGATGGTGTTGGTGACCGGGCCAACCGGTAGCGGTAAGACGGTGTCGCTGTACACCGGCTTGAACAT
TCTGAACAGCGTCGAGCGGAACATTTCGACCGCCGAAGATCCGGTCGAGATCACGGTCGAAGGTATCAATCAGGTCAACG
TCAATGCCAAAGCCGGCTTGACCTTCGCCGAAGCCTTGCGGGCTTTTTTGAGGCAAGACCCGGATATCATCATGGTCGGC
GAGATCCGCGACCTGGAAACCGCCAGCATCGCGGTCAAGGCAGCGCAAACCGGTCACTTGGTATTGTCGACCCTCCACAC
CAACGACGCGCCGCAAACCATCAATCGCTTGGTGCAGATGGGGATAGAGCCGTTCAATATCGTGTCGGCGGTGAATCTGA
TCATGGCCCAGCGCTTGGCGCGGCGGCTTTGCGAGCATTGCAAGAAGGAGGCCGACTTTCCGGAAAACATCTGGTTGTCG
GCCGGCTTTAGTAAGGAAGAAATCGGCACCTTCAAAATTTACGAACCGGTCGGTTGCGAGCATTGCAACAATGGCTACAA
GGGGCGGGTGGGTATCTATCAGGTGATGCCGATCAGCGAAGCGATGCGCGCGTTGATTTTGCGTGGCGGCAATGCCATGG
ACATGGCCGCGCTGTCCAAAACCGAAGGCATCAACGACCTTAGGGCTTCCGGCTTATTAAAAATACGGCAGGGTATTACG
TCGCTCGAAGAAATCGACCGCGTCACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.323

100

0.555
  pilB Acinetobacter baumannii D1279779

52.356

100

0.525
  pilB Acinetobacter baylyi ADP1

52.66

98.774

0.52
  pilF Neisseria gonorrhoeae MS11

48.208

97.723

0.471
  pilB Vibrio cholerae strain A1552

48.771

92.644

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

45.907

98.424

0.452
  pilB Vibrio campbellii strain DS40M4

45.133

98.949

0.447
  pilF Thermus thermophilus HB27

39.606

97.723

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.503

98.249

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

39.091

96.322

0.377

Multiple sequence alignment