Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACFWHB_RS02450 Genome accession   NZ_CP172298
Coordinates   483562..484803 (-) Length   413 a.a.
NCBI ID   WP_106885656.1    Uniprot ID   A0AAP4J3C9
Organism   Aeromonas sp. A-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478562..489803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFWHB_RS02425 (ACFWHB_02425) mutT 480535..480939 (+) 405 WP_395949381.1 8-oxo-dGTP diphosphatase MutT -
  ACFWHB_RS02430 (ACFWHB_02430) yacG 481012..481206 (-) 195 WP_025328449.1 DNA gyrase inhibitor YacG -
  ACFWHB_RS02435 (ACFWHB_02435) zapD 481216..481938 (-) 723 WP_162520171.1 cell division protein ZapD -
  ACFWHB_RS02440 (ACFWHB_02440) coaE 481976..482590 (-) 615 WP_395949382.1 dephospho-CoA kinase -
  ACFWHB_RS02445 (ACFWHB_02445) pilD 482609..483481 (-) 873 WP_395949383.1 prepilin peptidase Machinery gene
  ACFWHB_RS02450 (ACFWHB_02450) pilC 483562..484803 (-) 1242 WP_106885656.1 type II secretion system F family protein Machinery gene
  ACFWHB_RS02455 (ACFWHB_02455) pilB 484934..486640 (-) 1707 WP_395949384.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACFWHB_RS02460 (ACFWHB_02460) - 486644..487078 (-) 435 WP_395949385.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACFWHB_RS02465 (ACFWHB_02465) nadC 487391..488254 (-) 864 WP_395949386.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFWHB_RS02470 (ACFWHB_02470) - 488258..488728 (-) 471 WP_111911112.1 TIGR02281 family clan AA aspartic protease -
  ACFWHB_RS02475 (ACFWHB_02475) ampD 488865..489437 (+) 573 WP_111911110.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45345.33 Da        Isoelectric Point: 9.9044

>NTDB_id=1066098 ACFWHB_RS02450 WP_106885656.1 483562..484803(-) (pilC) [Aeromonas sp. A-8]
MATLAQKQNAPKKVFSFRWHGVNRKGQKVSGELQADSITTVKAELRKQGVNVTRVSKQGQGMFSKGGARIKPMDIAVISR
QITTMLSAGVPLVQSLQIIARGHEKAAVRELIGQIAADVETGTPLSDALRRHPRHFDALYCDLVEAGEQSGALETIYDRI
AIYREKSEALKSKIKKAMFYPAMVILVAIIVTSILLLFVIPQFEEIFKSFGAELPAFTRFIIAISRFMQDWWYAIFGGIA
LTVFLYVRAWRSSQKVRDNTDKFILSIPVVGNILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTATLAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVAAIFEQEVDDMVDGLTSLLEPIIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=1066098 ACFWHB_RS02450 WP_106885656.1 483562..484803(-) (pilC) [Aeromonas sp. A-8]
ATGGCTACATTAGCTCAAAAACAAAACGCACCAAAAAAGGTCTTCTCCTTCCGCTGGCACGGCGTCAATCGCAAGGGACA
AAAGGTTTCCGGTGAGTTGCAAGCCGACAGCATCACGACAGTCAAGGCGGAGCTGCGCAAGCAGGGCGTCAATGTCACCA
GGGTCAGCAAGCAGGGCCAGGGAATGTTTTCCAAGGGCGGCGCCAGGATCAAGCCGATGGACATCGCCGTCATCTCCCGC
CAGATCACCACCATGCTCTCTGCCGGCGTGCCGCTGGTGCAGAGCCTGCAGATCATTGCCCGTGGCCATGAAAAGGCGGC
GGTGCGCGAGCTGATCGGCCAGATCGCCGCCGATGTGGAGACAGGCACCCCCCTGTCAGATGCGCTGCGCCGCCACCCCC
GCCACTTCGATGCCCTCTACTGTGACCTGGTGGAGGCGGGGGAGCAGTCAGGCGCGCTGGAAACCATCTACGACCGGATT
GCCATCTATCGCGAGAAGAGCGAGGCGCTCAAATCCAAGATCAAGAAGGCCATGTTCTATCCCGCCATGGTCATCCTGGT
CGCCATCATAGTGACCTCCATACTGTTGCTGTTCGTCATCCCCCAGTTCGAAGAGATCTTCAAGAGCTTCGGGGCCGAGC
TGCCCGCCTTCACCCGATTCATCATTGCCATCTCCCGCTTCATGCAGGATTGGTGGTACGCCATCTTCGGCGGCATAGCC
CTGACAGTATTCCTCTATGTGCGCGCCTGGCGCAGCTCCCAGAAGGTACGTGACAACACCGACAAGTTCATCCTCTCCAT
TCCAGTCGTTGGCAACATACTGCACAAGGCGGCCATGGCCCGTTTCGCCCGCACCCTCTCCACCACCTTCTCCGCCGGTA
TTCCCCTGGTGGATGCCCTGGTCTCCGCTGCCGGGGCCTCGGGCAACTATGTCTATCGCACGGCTACCCTGGCCATTCGC
AACGAAGTCGTGGCTGGCATGCAGATCAACGTCGCCATGCGCACCGTGGATCTTTTCCCCGACATGGTGATCCAGATGGT
GATGATAGGTGAAGAGTCCGGCGCCATCGACGACATGCTCTCCAAGGTCGCCGCCATCTTCGAGCAGGAGGTGGACGACA
TGGTCGATGGTCTCACCAGCCTGCTCGAGCCCATCATAATGGTGGTGCTGGGGGTGCTGGTCGGCGGCATGGTCGTCGCC
ATGTACCTCCCCATCTTCAAGCTGGGCTCTGTGATACACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.303

95.884

0.53
  pilC Legionella pneumophila strain ERS1305867

51.605

98.063

0.506
  pilC Acinetobacter baumannii D1279779

51.87

97.094

0.504
  pilC Acinetobacter baylyi ADP1

50.882

96.126

0.489
  pilC Vibrio cholerae strain A1552

46.192

98.547

0.455
  pilC Vibrio campbellii strain DS40M4

44.802

97.821

0.438
  pilG Neisseria meningitidis 44/76-A

40.247

98.063

0.395
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilC Thermus thermophilus HB27

38

96.852

0.368

Multiple sequence alignment