Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFWHB_RS02455 Genome accession   NZ_CP172298
Coordinates   484934..486640 (-) Length   568 a.a.
NCBI ID   WP_395949384.1    Uniprot ID   -
Organism   Aeromonas sp. A-8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 479934..491640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFWHB_RS02425 (ACFWHB_02425) mutT 480535..480939 (+) 405 WP_395949381.1 8-oxo-dGTP diphosphatase MutT -
  ACFWHB_RS02430 (ACFWHB_02430) yacG 481012..481206 (-) 195 WP_025328449.1 DNA gyrase inhibitor YacG -
  ACFWHB_RS02435 (ACFWHB_02435) zapD 481216..481938 (-) 723 WP_162520171.1 cell division protein ZapD -
  ACFWHB_RS02440 (ACFWHB_02440) coaE 481976..482590 (-) 615 WP_395949382.1 dephospho-CoA kinase -
  ACFWHB_RS02445 (ACFWHB_02445) pilD 482609..483481 (-) 873 WP_395949383.1 prepilin peptidase Machinery gene
  ACFWHB_RS02450 (ACFWHB_02450) pilC 483562..484803 (-) 1242 WP_106885656.1 type II secretion system F family protein Machinery gene
  ACFWHB_RS02455 (ACFWHB_02455) pilB 484934..486640 (-) 1707 WP_395949384.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACFWHB_RS02460 (ACFWHB_02460) - 486644..487078 (-) 435 WP_395949385.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACFWHB_RS02465 (ACFWHB_02465) nadC 487391..488254 (-) 864 WP_395949386.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFWHB_RS02470 (ACFWHB_02470) - 488258..488728 (-) 471 WP_111911112.1 TIGR02281 family clan AA aspartic protease -
  ACFWHB_RS02475 (ACFWHB_02475) ampD 488865..489437 (+) 573 WP_111911110.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACFWHB_RS02480 (ACFWHB_02480) pdhR 489713..490477 (+) 765 WP_106885661.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62564.93 Da        Isoelectric Point: 5.8194

>NTDB_id=1066099 ACFWHB_RS02455 WP_395949384.1 484934..486640(-) (pilB) [Aeromonas sp. A-8]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAALGKLMDTEQDTLGMDHIDEAEI
SELEVSDEGSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRSRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNLDQLGFDDRQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPDAELQELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1066099 ACFWHB_RS02455 WP_395949384.1 484934..486640(-) (pilB) [Aeromonas sp. A-8]
ATGACCTCTAGCCTCAATAGCGGCCTGGCGACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAGAATGACATCCTCGACAGCAAGGCCCTCG
CGGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTTATCGAGAAGCATCATGTGTTGCCCATCTATACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACGGAAGCCTTGCTGGTTGAGGAGAGCA
AGCTCACCGCCGCCCTCGGCAAGCTGATGGACACCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAGATC
TCGGAGCTCGAGGTTTCCGATGAGGGTTCGCGCCTCGACGAGAGCGTCAATACCGCCGACGACGATGCCCCCATAGTCAA
ATACATCCATAAAATCATGATGGATGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACA
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCCCGT
CTCAAGGTGATGGCGAGACTCGATATAGCCGAGCGACGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCACGCAG
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGTGAGAAGATAGTCATCCGCTTGCTGGACTCGTCAG
CTGCCCGCCTCAACCTCGATCAGCTCGGCTTCGACGACCGCCAGAAGGCGCAATACCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACTGGCTCGGGCAAGACGGTGTCGCTCTATACAGGTCTCAACATTCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGACCCGGTGGAGATCAATCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTACGCCAGGATCCCGACATCGTCATGGTGGGGGAAATACGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCCCAGACAGGTCACTTGGTACTATCAACCCTGCACACCAACTCGGCAGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAAC
GTCTCGCCCGCAAGCTGTGCGATCACTGCAAGGCCCCCGAGGTGGTGCCCGACGCCGAACTGCAAGAGCTGGGCTTTACC
CAACAGCAGCTGGCTGCCGGACTGCGGCTGTTCAAGCCGGTCGGCTGCAAGGAGTGCTCGGGAGGATACAGAGGGCGGGT
CGGCATCTATGAAATCATGCTGATGTCGGACAACATCGCCAAGCTGATCATGCAGGGAGCGAACTCGCTGCAAATCGCGG
CCATCGCCCAGAAGGAGGGCATGCGCACGCTACGCATCTCCGGCCTGGAGAAAGCCCGTTTGGGAGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.203

99.824

0.551
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Acinetobacter baylyi ADP1

54.982

98.944

0.544
  pilB Vibrio cholerae strain A1552

52.931

99.12

0.525
  pilB Vibrio campbellii strain DS40M4

50.353

99.648

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

49.556

99.12

0.491
  pilF Neisseria gonorrhoeae MS11

46.208

99.824

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.087

97.535

0.371
  pilF Thermus thermophilus HB27

39.962

92.958

0.371

Multiple sequence alignment