Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   ACG4EF_RS03475 Genome accession   NZ_CP172259
Coordinates   704043..704468 (+) Length   141 a.a.
NCBI ID   WP_397321275.1    Uniprot ID   -
Organism   Pediococcus acidilactici strain XJ-24     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 694704..739717 704043..704468 within 0


Gene organization within MGE regions


Location: 694704..739717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG4EF_RS03400 (ACG4EF_03400) - 694704..695393 (-) 690 WP_201197918.1 N-acetylmannosamine-6-phosphate 2-epimerase -
  ACG4EF_RS03405 (ACG4EF_03405) - 695788..696867 (-) 1080 WP_397321268.1 tyrosine-type recombinase/integrase -
  ACG4EF_RS03410 (ACG4EF_03410) - 697006..697920 (-) 915 WP_201197919.1 DUF5067 domain-containing protein -
  ACG4EF_RS03415 (ACG4EF_03415) - 697939..698583 (-) 645 WP_201197920.1 hypothetical protein -
  ACG4EF_RS03420 (ACG4EF_03420) - 698645..699055 (-) 411 WP_201197921.1 hypothetical protein -
  ACG4EF_RS03425 (ACG4EF_03425) - 699059..699397 (-) 339 WP_236252657.1 XRE family transcriptional regulator -
  ACG4EF_RS03430 (ACG4EF_03430) - 699661..699849 (+) 189 WP_201197922.1 helix-turn-helix domain-containing protein -
  ACG4EF_RS03435 (ACG4EF_03435) - 699862..700317 (+) 456 WP_201197923.1 hypothetical protein -
  ACG4EF_RS03440 (ACG4EF_03440) - 700336..701070 (+) 735 WP_397321269.1 Rha family transcriptional regulator -
  ACG4EF_RS03445 (ACG4EF_03445) - 701139..701345 (+) 207 WP_075139784.1 hypothetical protein -
  ACG4EF_RS03450 (ACG4EF_03450) - 701440..702522 (+) 1083 WP_397321270.1 N-6 DNA methylase -
  ACG4EF_RS03455 (ACG4EF_03455) - 702528..702707 (+) 180 WP_397321271.1 hypothetical protein -
  ACG4EF_RS03460 (ACG4EF_03460) - 702697..703152 (+) 456 WP_397321272.1 chromosome segregation protein SMC -
  ACG4EF_RS03465 (ACG4EF_03465) - 703153..703851 (+) 699 WP_397321273.1 ERF family protein -
  ACG4EF_RS03470 (ACG4EF_03470) - 703844..704050 (+) 207 WP_397321274.1 hypothetical protein -
  ACG4EF_RS03475 (ACG4EF_03475) ssb 704043..704468 (+) 426 WP_397321275.1 single-stranded DNA-binding protein Machinery gene
  ACG4EF_RS03480 (ACG4EF_03480) - 704480..705178 (+) 699 WP_397321276.1 putative HNHc nuclease -
  ACG4EF_RS03485 (ACG4EF_03485) - 705159..705923 (+) 765 WP_397321277.1 conserved phage C-terminal domain-containing protein -
  ACG4EF_RS03490 (ACG4EF_03490) - 705937..706719 (+) 783 WP_397321278.1 ATP-binding protein -
  ACG4EF_RS03495 (ACG4EF_03495) - 706837..707469 (+) 633 WP_397321279.1 RNA polymerase subunit sigma-70 -
  ACG4EF_RS03500 (ACG4EF_03500) - 707450..707851 (+) 402 WP_397321280.1 RusA family crossover junction endodeoxyribonuclease -
  ACG4EF_RS03505 (ACG4EF_03505) - 707848..707997 (+) 150 WP_397321281.1 hypothetical protein -
  ACG4EF_RS03510 (ACG4EF_03510) - 707984..708355 (+) 372 WP_397321282.1 N-acetylmuramoyl-L-alanine amidase -
  ACG4EF_RS03515 (ACG4EF_03515) - 708525..708758 (+) 234 WP_397321283.1 hypothetical protein -
  ACG4EF_RS03520 (ACG4EF_03520) - 708748..709086 (+) 339 WP_330156159.1 hypothetical protein -
  ACG4EF_RS03525 (ACG4EF_03525) - 709079..709399 (+) 321 WP_330156160.1 hypothetical protein -
  ACG4EF_RS03530 (ACG4EF_03530) - 709456..709869 (+) 414 WP_330156161.1 hypothetical protein -
  ACG4EF_RS03535 (ACG4EF_03535) - 709899..710207 (+) 309 WP_397321284.1 hypothetical protein -
  ACG4EF_RS03540 (ACG4EF_03540) - 710207..710464 (+) 258 WP_397321285.1 hypothetical protein -
  ACG4EF_RS03545 (ACG4EF_03545) - 710464..710802 (+) 339 WP_075139800.1 hypothetical protein -
  ACG4EF_RS03550 (ACG4EF_03550) - 710906..711349 (+) 444 WP_397321286.1 hypothetical protein -
  ACG4EF_RS03555 (ACG4EF_03555) - 711886..712377 (+) 492 WP_397321287.1 terminase small subunit -
  ACG4EF_RS03560 (ACG4EF_03560) - 712370..713716 (+) 1347 WP_397321288.1 PBSX family phage terminase large subunit -
  ACG4EF_RS03565 (ACG4EF_03565) - 713798..715132 (+) 1335 WP_397321289.1 phage portal protein -
  ACG4EF_RS03570 (ACG4EF_03570) - 715101..716084 (+) 984 WP_397321290.1 minor capsid protein -
  ACG4EF_RS03575 (ACG4EF_03575) - 716188..716907 (+) 720 WP_397321291.1 DUF4355 domain-containing protein -
  ACG4EF_RS03580 (ACG4EF_03580) - 716907..717722 (+) 816 WP_397321292.1 N4-gp56 family major capsid protein -
  ACG4EF_RS03585 (ACG4EF_03585) - 717740..718006 (+) 267 WP_397321341.1 Ig-like domain-containing protein -
  ACG4EF_RS03590 (ACG4EF_03590) - 718018..718392 (+) 375 WP_397321293.1 phage head-tail connector protein -
  ACG4EF_RS03595 (ACG4EF_03595) - 718397..718702 (+) 306 WP_397321294.1 hypothetical protein -
  ACG4EF_RS03600 (ACG4EF_03600) - 718695..719036 (+) 342 WP_285110031.1 HK97-gp10 family putative phage morphogenesis protein -
  ACG4EF_RS03605 (ACG4EF_03605) - 719037..719438 (+) 402 WP_397321295.1 hypothetical protein -
  ACG4EF_RS03610 (ACG4EF_03610) - 719455..720060 (+) 606 WP_397321296.1 phage major tail protein, TP901-1 family -
  ACG4EF_RS03615 (ACG4EF_03615) - 720075..720581 (+) 507 WP_397321297.1 Ig domain-containing protein -
  ACG4EF_RS03620 (ACG4EF_03620) - 720644..720979 (+) 336 WP_397321298.1 tail assembly chaperone -
  ACG4EF_RS03625 (ACG4EF_03625) - 721060..721395 (+) 336 WP_397321299.1 hypothetical protein -
  ACG4EF_RS03630 (ACG4EF_03630) - 721379..724399 (+) 3021 WP_397321300.1 phage tail tape measure protein -
  ACG4EF_RS03635 (ACG4EF_03635) - 724399..725160 (+) 762 WP_397321301.1 phage tail protein -
  ACG4EF_RS03640 (ACG4EF_03640) - 725160..728105 (+) 2946 WP_397321302.1 phage tail protein -
  ACG4EF_RS03645 (ACG4EF_03645) - 728105..728518 (+) 414 WP_075139818.1 hypothetical protein -
  ACG4EF_RS03650 (ACG4EF_03650) - 728522..730258 (+) 1737 WP_397321303.1 BppU family phage baseplate upper protein -
  ACG4EF_RS03655 (ACG4EF_03655) - 730251..731210 (+) 960 WP_397321304.1 hypothetical protein -
  ACG4EF_RS03660 (ACG4EF_03660) - 731210..731488 (+) 279 WP_075139821.1 hypothetical protein -
  ACG4EF_RS03665 (ACG4EF_03665) - 731488..731634 (+) 147 WP_075139822.1 XkdX family protein -
  ACG4EF_RS03670 (ACG4EF_03670) - 731585..731968 (+) 384 WP_229092876.1 hypothetical protein -
  ACG4EF_RS03675 (ACG4EF_03675) - 731968..732210 (+) 243 WP_128211673.1 phage holin -
  ACG4EF_RS03680 (ACG4EF_03680) - 732194..733315 (+) 1122 WP_201197725.1 peptidoglycan recognition family protein -
  ACG4EF_RS03685 (ACG4EF_03685) - 734060..734908 (+) 849 WP_397321342.1 ROK family protein -
  ACG4EF_RS03690 (ACG4EF_03690) - 734889..735620 (+) 732 WP_397321305.1 sugar phosphate isomerase/epimerase family protein -
  ACG4EF_RS03695 (ACG4EF_03695) - 735874..737382 (+) 1509 WP_004165605.1 sodium:solute symporter -
  ACG4EF_RS03700 (ACG4EF_03700) - 737397..738314 (+) 918 WP_201197959.1 dihydrodipicolinate synthase family protein -
  ACG4EF_RS03705 (ACG4EF_03705) - 739361..739717 (+) 357 WP_236252674.1 AP2 domain-containing protein -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15679.41 Da        Isoelectric Point: 7.1664

>NTDB_id=1065963 ACG4EF_RS03475 WP_397321275.1 704043..704468(+) (ssb) [Pediococcus acidilactici strain XJ-24]
MINRTVLIGRLTKDVELRHTAKGDAVASFTVAVNRQFTNSQGEREADFINCVMWRKAAENFAKYTHKGSLVGIEGRIQTR
SYENQQGQRVYVTEVVADNFSLLDSKPKGNQQNNVRQSTTPGDPFANGGQSIDIGDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1065963 ACG4EF_RS03475 WP_397321275.1 704043..704468(+) (ssb) [Pediococcus acidilactici strain XJ-24]
ATGATTAATCGAACAGTACTAATAGGACGTTTAACTAAAGATGTTGAGCTTCGCCACACAGCTAAAGGTGATGCGGTAGC
TAGTTTTACCGTGGCAGTTAACCGACAGTTTACCAACTCACAGGGTGAACGTGAAGCGGATTTCATCAACTGTGTAATGT
GGCGGAAAGCGGCGGAAAACTTTGCTAAGTATACACACAAGGGTTCGTTGGTAGGTATTGAAGGGCGGATTCAAACCCGT
TCGTACGAAAATCAACAAGGGCAACGAGTTTATGTAACTGAGGTTGTAGCTGATAACTTCTCACTGCTAGATTCGAAACC
AAAAGGCAACCAGCAAAATAACGTACGGCAATCTACAACGCCGGGAGACCCGTTCGCTAATGGCGGGCAGTCAATCGATA
TTGGTGACGACGATTTACCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

60

100

0.723

  ssbA Bacillus subtilis subsp. subtilis str. 168

53.488

100

0.652

  ssbB Bacillus subtilis subsp. subtilis str. 168

61.111

76.596

0.468

  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.135

100

0.411

  ssbB/cilA Streptococcus mitis NCTC 12261

40.278

100

0.411

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.278

100

0.411

  ssbB/cilA Streptococcus pneumoniae Rx1

39.583

100

0.404

  ssbB/cilA Streptococcus pneumoniae D39

39.583

100

0.404

  ssbB/cilA Streptococcus pneumoniae R6

39.583

100

0.404

  ssbB/cilA Streptococcus mitis SK321

39.583

100

0.404

  ssbA Streptococcus mutans UA159

37.589

100

0.376


Multiple sequence alignment