Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACH105_RS18435 Genome accession   NZ_CP172234
Coordinates   3925326..3926471 (-) Length   381 a.a.
NCBI ID   WP_041108398.1    Uniprot ID   A0A8D3Y4G4
Organism   Stutzerimonas balearica strain Y11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3920326..3931471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH105_RS18410 (ACH105_18410) - 3920442..3921464 (+) 1023 WP_041108389.1 aspartate carbamoyltransferase catalytic subunit -
  ACH105_RS18415 (ACH105_18415) - 3921458..3922729 (+) 1272 WP_395764077.1 dihydroorotase -
  ACH105_RS18420 (ACH105_18420) - 3922767..3923168 (-) 402 WP_041108393.1 TM2 domain-containing protein -
  ACH105_RS18425 (ACH105_18425) - 3923265..3924476 (+) 1212 WP_395764078.1 putative DNA modification/repair radical SAM protein -
  ACH105_RS18430 (ACH105_18430) - 3924476..3925360 (+) 885 WP_395764079.1 TIGR03915 family putative DNA repair protein -
  ACH105_RS18435 (ACH105_18435) pilU 3925326..3926471 (-) 1146 WP_041108398.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACH105_RS18440 (ACH105_18440) pilT 3926548..3927582 (-) 1035 WP_043222415.1 type IV pilus twitching motility protein PilT Machinery gene
  ACH105_RS18445 (ACH105_18445) - 3927648..3928337 (+) 690 WP_041108402.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACH105_RS18450 (ACH105_18450) proC 3928357..3929190 (+) 834 WP_041108404.1 pyrroline-5-carboxylate reductase -
  ACH105_RS18455 (ACH105_18455) - 3929200..3929790 (+) 591 WP_041108406.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42517.82 Da        Isoelectric Point: 6.6647

>NTDB_id=1065790 ACH105_RS18435 WP_041108398.1 3925326..3926471(-) (pilU) [Stutzerimonas balearica strain Y11]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLEDLKLPDVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNTSGHIISIED
PIEFIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYNLYVQGEITYEDALAHADSANDLRLMIKLGSETDSEHLTSVSQGLRLEVSDDDPGFRMR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1065790 ACH105_RS18435 WP_041108398.1 3925326..3926471(-) (pilU) [Stutzerimonas balearica strain Y11]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAGAAGGGCGGCTCCGACCTGTTCATCACGGCCGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTCAGCAAGACGCCGATGTCGCCGGAGATGACCCGCGAAACCGTGCATGGGG
TGATGAACGAGCAGCAGCGTCGCGAGTTCACCGAGAACCACGAGTGCAATTTCGCCATCAGCGCTCGGGGGATCGGGCGT
TTCCGGGTCAGTGCGTTCTACCAGCGCAACCTCGCCGGCATGGTCCTGCGGCGGATCGAGACCAACATCCCCACGCTCGA
AGACCTCAAACTGCCCGACGTGTTGAAGAAGCTGGCGCTGACCAAGCGGGGGCTGGTGCTGTTCGTCGGGGCGACCGGTA
CTGGCAAGTCCACGTCGCTGGCGGCAATGATTGGCTACCGCAACAAGAACACCAGTGGCCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAGCTGCATCGTGACTCAGCGCGAGGTGGGCATCGATACGGAGTCGTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCTGACGTCATCCTCATCGGCGAAGTGCGGACCCGCGAGACGATGGATTATG
CCGTGGCGTTCGCCGAGACCGGCCACTTGTGCCTGGCCACGCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGCTGATCGGCAGCAGCAGGTATGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACACCCGACGGCAAGGGGCGGCGAGCGGTGATCGAGGTGCTGATCAACACTCCGCTGGCCGCCGACCTGA
TCCGCAAAGGCGAGGTACATGAGCTCAAGGGGCTGATGAAGCGATCCACCGAGCAAGGCATGCAGACCTTCGATCAGGCG
CTCTACAACCTCTATGTGCAAGGCGAGATCACCTACGAGGATGCCTTGGCTCATGCCGATTCGGCCAACGATCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGACAGCGAGCACCTCACCAGCGTGTCCCAGGGGCTGCGCCTGGAAGTCAGTGACG
ACGATCCGGGCTTCCGGATGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

95.276

100

0.953
  pilU Acinetobacter baylyi ADP1

65.546

93.701

0.614
  pilU Vibrio cholerae strain A1552

60

91.864

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

88.976

0.386
  pilT Legionella pneumophila strain Lp02

41.493

87.927

0.365
  pilT Legionella pneumophila strain ERS1305867

41.493

87.927

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.124

88.714

0.365
  pilT Vibrio cholerae strain A1552

41.124

88.714

0.365
  pilT Neisseria gonorrhoeae MS11

40

90.551

0.362
  pilT Neisseria meningitidis 8013

40

90.551

0.362

Multiple sequence alignment