Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHWYH_RS05740 Genome accession   NZ_CP172084
Coordinates   1158208..1159602 (-) Length   464 a.a.
NCBI ID   WP_112103477.1    Uniprot ID   -
Organism   Haemophilus influenzae strain GA81666     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1153208..1164602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWYH_RS05705 - 1153446..1153652 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  ACHWYH_RS05710 - 1153738..1153944 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  ACHWYH_RS05715 cueR 1154021..1154407 (+) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  ACHWYH_RS05720 metJ 1154421..1154738 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  ACHWYH_RS05725 rho 1154986..1156248 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  ACHWYH_RS05730 pilD 1156302..1156994 (-) 693 WP_112103479.1 A24 family peptidase Machinery gene
  ACHWYH_RS05735 pilC 1156991..1158211 (-) 1221 WP_112103478.1 type II secretion system F family protein Machinery gene
  ACHWYH_RS05740 pilB 1158208..1159602 (-) 1395 WP_112103477.1 GspE/PulE family protein Machinery gene
  ACHWYH_RS05745 pilA 1159599..1160048 (-) 450 WP_110426612.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACHWYH_RS05750 ampD 1160163..1160723 (+) 561 WP_112103476.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACHWYH_RS05755 corC 1161346..1162245 (+) 900 WP_110426610.1 CNNM family magnesium/cobalt transport protein CorC -
  ACHWYH_RS05760 lnt 1162268..1163785 (+) 1518 WP_080279450.1 apolipoprotein N-acyltransferase -
  ACHWYH_RS05765 rsmE 1163835..1164572 (+) 738 WP_112103475.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52895.25 Da        Isoelectric Point: 5.6399

>NTDB_id=1065049 ACHWYH_RS05740 WP_112103477.1 1158208..1159602(-) (pilB) [Haemophilus influenzae strain GA81666]
MTSYALLHTQRVTAQNGEIFTISPDLWVRNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTESQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINSQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVISTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1065049 ACHWYH_RS05740 WP_112103477.1 1158208..1159602(-) (pilB) [Haemophilus influenzae strain GA81666]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATATTTACGATCTCGCCTGATTTATG
GGTGCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAGGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGTCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACTGAAAGCCAACAACAGGCATTTCAACACGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCACAGG
AAGTGGGAAAAGTATCTCACTTTACACTGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACAGCGGAAGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATTCACAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGGCATTTGGTGATTTCAACTTTACATACCAATGATGCAATTTCTGCTATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAACGTCTTGTACGAAAGCTTTGTTCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGTATAACAGATGAGA
AAGAAATTGAGCGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

97.845

100

0.978
  pilB Haemophilus influenzae Rd KW20

96.112

99.784

0.959
  pilB Glaesserella parasuis strain SC1401

57.895

98.276

0.569
  pilB Vibrio cholerae strain A1552

39.848

100

0.453
  pilB Vibrio campbellii strain DS40M4

40.286

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.416
  pilB Acinetobacter baylyi ADP1

38.508

100

0.412
  pilB Acinetobacter baumannii D1279779

39.085

100

0.405
  pilF Neisseria gonorrhoeae MS11

37.395

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.853

100

0.384
  pilF Thermus thermophilus HB27

36.842

100

0.377

Multiple sequence alignment