Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACHWYH_RS05745 Genome accession   NZ_CP172084
Coordinates   1159599..1160048 (-) Length   149 a.a.
NCBI ID   WP_110426612.1    Uniprot ID   -
Organism   Haemophilus influenzae strain GA81666     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1154599..1165048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWYH_RS05725 rho 1154986..1156248 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  ACHWYH_RS05730 pilD 1156302..1156994 (-) 693 WP_112103479.1 A24 family peptidase Machinery gene
  ACHWYH_RS05735 pilC 1156991..1158211 (-) 1221 WP_112103478.1 type II secretion system F family protein Machinery gene
  ACHWYH_RS05740 pilB 1158208..1159602 (-) 1395 WP_112103477.1 GspE/PulE family protein Machinery gene
  ACHWYH_RS05745 pilA 1159599..1160048 (-) 450 WP_110426612.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACHWYH_RS05750 ampD 1160163..1160723 (+) 561 WP_112103476.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACHWYH_RS05755 corC 1161346..1162245 (+) 900 WP_110426610.1 CNNM family magnesium/cobalt transport protein CorC -
  ACHWYH_RS05760 lnt 1162268..1163785 (+) 1518 WP_080279450.1 apolipoprotein N-acyltransferase -
  ACHWYH_RS05765 rsmE 1163835..1164572 (+) 738 WP_112103475.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15542.90 Da        Isoelectric Point: 8.4607

>NTDB_id=1065050 ACHWYH_RS05745 WP_110426612.1 1159599..1160048(-) (pilA) [Haemophilus influenzae strain GA81666]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYKNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITTAKGYVASVKTQSGGITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1065050 ACHWYH_RS05745 WP_110426612.1 1159599..1160048(-) (pilA) [Haemophilus influenzae strain GA81666]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGTTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATCCCCTCTTATAAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGTACAAATGAAACAACAAATTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCACAGCAAAAGGCTATGTAGCCTCAGTAAAAACGCAATCAGGTGGCATTACCGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

95.973

100

0.96
  pilA Haemophilus influenzae Rd KW20

93.96

100

0.94
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila strain ERS1305867

39.456

98.658

0.389
  pilA Vibrio campbellii strain DS40M4

40.426

94.631

0.383
  pilA2 Legionella pneumophila str. Paris

39.568

93.289

0.369

Multiple sequence alignment