Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACHFJ9_RS21395 Genome accession   NZ_CP171887
Coordinates   2891356..2893104 (-) Length   582 a.a.
NCBI ID   WP_006742777.1    Uniprot ID   A0AA92LSF7
Organism   Vibrio sp. D3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2886356..2898104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFJ9_RS21375 (ACHFJ9_21375) - 2887041..2887880 (-) 840 WP_005395710.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACHFJ9_RS21380 (ACHFJ9_21380) - 2887950..2889464 (-) 1515 WP_258623047.1 SPOR domain-containing protein -
  ACHFJ9_RS21385 (ACHFJ9_21385) aroB 2889517..2890617 (-) 1101 WP_005395712.1 3-dehydroquinate synthase -
  ACHFJ9_RS21390 (ACHFJ9_21390) aroK 2890650..2891168 (-) 519 WP_005381319.1 shikimate kinase AroK -
  ACHFJ9_RS21395 (ACHFJ9_21395) pilQ 2891356..2893104 (-) 1749 WP_006742777.1 type IV pilus secretin PilQ Machinery gene
  ACHFJ9_RS21400 (ACHFJ9_21400) pilP 2893130..2893645 (-) 516 WP_237312731.1 pilus assembly protein PilP Machinery gene
  ACHFJ9_RS21405 (ACHFJ9_21405) pilO 2893635..2894228 (-) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACHFJ9_RS21410 (ACHFJ9_21410) pilN 2894221..2894796 (-) 576 WP_046874634.1 PilN domain-containing protein Machinery gene
  ACHFJ9_RS21415 (ACHFJ9_21415) pilM 2894780..2895796 (-) 1017 WP_301610125.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63760.73 Da        Isoelectric Point: 4.4533

>NTDB_id=1064664 ACHFJ9_RS21395 WP_006742777.1 2891356..2893104(-) (pilQ) [Vibrio sp. D3]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLVAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDVKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYTHDYRLTGRFLEIKVAKIKPNEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQQLEQTMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYNGGGEGEDGGDGDGVGIDDFLNVNLAATSPNA
TSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=1064664 ACHFJ9_RS21395 WP_006742777.1 2891356..2893104(-) (pilQ) [Vibrio sp. D3]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTAACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGTGGCAGCAGAAGAAGTATCGTCTAATGCCTTAAAGAGTATCGATTTTCGCACTAATAAAGATAAAGACGCCG
TTATTGTCGTTGAACTGGCGTCACCTGCCGCGATTGTTGATGTCAAACGCGTACAGGAAGGGTTAAGTATCGATTTGCTT
AATACTTCGGTAAAAGACGAGCAACTGTATCTCCTAGACGTAAAAGATTTCTCGACTGTGGTTGAAAGCGTAGAGGTGTT
TCGCGATACCTCAACTACGCGCTTGGTTGCGCACATCAATGAAGAATATACGCATGATTACCGTTTAACGGGGCGATTCT
TGGAAATCAAAGTGGCTAAGATCAAACCCAATGAGAAAGCTCCGGATAAAAGCATTCTAGAAAAAGAAGGCAAGCTCATT
TCGATTAACTTCCAAGATATTCCTGTACGTAACGTGTTACAGCTGATTGCCGACTACAACCAGTTCAACTTGGTTGTTTC
AGACTCCGTAGAAGGTAACCTCACACTTCGTCTTGATGGTGTTCCGTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTCTGGTTGCTCCAACAGATGAGCTTGATCTGCGCGAAAAACAGCAA
CTCGAAAAGCAGCAGCTAGAACAAACGATGGGAGAGCTTTCGTCTGAAATCATCAAGGTAAACTTCGCTAAAGCATCAGA
TATTGCAGAGATGATCAACGGAGAAGGGAACATCAGTATGTTGTCTGAGCGAGGCAGTATGACCATTGATGAGCGGACTA
ACTCGTTATTGATTCGTGAGCTACCTGAAAACATTGAAGTGATTCGAGAAATTATTGAATCTTTGGATATCCCGGTGAAG
CAGGTTCAAATTGAAGCGCGTATTGTGACCATCAATGAAGGCAACATGGACGAGTTGGGAATTCGCTGGGGCTTTACCTC
TATTAATGGCAATAACACGGTTGGTGGTTCTATCGAAAACAACCTTGCCACGATCGGTTTATATAACGGTGGTGGTGAAG
GAGAAGACGGTGGTGACGGTGATGGAGTAGGTATCGATGACTTCCTAAACGTAAACTTAGCTGCAACCAGTCCAAACGCG
ACCAGCATTGCTTTTCAGGTTGCAAAATTAGGCTCAGACACCTTGTTAGACCTCGAGCTTTCTGCGCTGCAACAAGAGTC
CAAAGCTGAGATTATCTCAAGCCCACGTTTGATCACTACTAATAAGAAGCCTGCCTATATTGAGCAAGGTACCGAGATAC
CTTATCTAGAGTCCTCATCAAGTGGTGCCACGACGGTAACGTTCAAAAAAGCAGTATTGAGTTTGAAGGTGACGCCACAA
ATTACACCGGATAACCGTCTTGTGTTGGACTTAAGCGTTACGCAAGATAGACCTGGACAAGTCGTGAAAACAGGCACTGG
TGAAGCCGTTGCGATTGATACTCAGCGTATCGGGACGCAAGTGTTAGTAAATAACGGTGAAACCGTCGTTCTTGGTGGTA
TTTTCCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTAGGCGATCTGCCACTATTAGGTGCGCTTTTCCGT
CGAAGTTATGAAAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACCCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.955

100

0.93
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.443

100

0.746
  pilQ Vibrio cholerae strain A1552

74.443

100

0.746
  pilQ Legionella pneumophila strain Lp02

40.787

96.048

0.392
  pilQ Legionella pneumophila strain ERS1305867

40.787

96.048

0.392
  pilQ Pseudomonas aeruginosa PAK

39.716

96.907

0.385
  comQ Acinetobacter baylyi ADP1

37.349

99.828

0.373
  pilQ Acinetobacter baumannii strain A118

36.409

100

0.373
  pilQ Acinetobacter baumannii D1279779

36.409

100

0.373

Multiple sequence alignment