Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACHFJ9_RS21405 Genome accession   NZ_CP171887
Coordinates   2893635..2894228 (-) Length   197 a.a.
NCBI ID   WP_012841351.1    Uniprot ID   A0AAE4SAG9
Organism   Vibrio sp. D3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2888635..2899228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFJ9_RS21385 (ACHFJ9_21385) aroB 2889517..2890617 (-) 1101 WP_005395712.1 3-dehydroquinate synthase -
  ACHFJ9_RS21390 (ACHFJ9_21390) aroK 2890650..2891168 (-) 519 WP_005381319.1 shikimate kinase AroK -
  ACHFJ9_RS21395 (ACHFJ9_21395) pilQ 2891356..2893104 (-) 1749 WP_006742777.1 type IV pilus secretin PilQ Machinery gene
  ACHFJ9_RS21400 (ACHFJ9_21400) pilP 2893130..2893645 (-) 516 WP_237312731.1 pilus assembly protein PilP Machinery gene
  ACHFJ9_RS21405 (ACHFJ9_21405) pilO 2893635..2894228 (-) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACHFJ9_RS21410 (ACHFJ9_21410) pilN 2894221..2894796 (-) 576 WP_046874634.1 PilN domain-containing protein Machinery gene
  ACHFJ9_RS21415 (ACHFJ9_21415) pilM 2894780..2895796 (-) 1017 WP_301610125.1 type IV pilus biogenesis protein PilM Machinery gene
  ACHFJ9_RS21420 (ACHFJ9_21420) - 2895940..2898501 (+) 2562 WP_395356240.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23042.45 Da        Isoelectric Point: 4.7906

>NTDB_id=1064666 ACHFJ9_RS21405 WP_012841351.1 2893635..2894228(-) (pilO) [Vibrio sp. D3]
MVDWQDLEIDEMAEWPLLPQLVVIALLIFLIQGFGYWFYLKPKEEQLNNLVQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETNNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=1064666 ACHFJ9_RS21405 WP_012841351.1 2893635..2894228(-) (pilO) [Vibrio sp. D3]
ATGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCAGAGTGGCCATTGTTGCCTCAGCTTGTGGTTATCGCGCTGCT
GATTTTCCTCATTCAAGGTTTTGGCTATTGGTTTTACCTTAAGCCAAAAGAAGAGCAATTGAATAACTTGGTCCAGCAGG
AGCAGACGCTCAAGGTAGCACTGAAGATCAAAGCGAACAAAGTAGCTGCATTGCCTCAGCTTCAATCTCAATTGGATGAA
CTCACCACGCGTTACGAGTTATTGCTACAACAACTGCCCGCGCAAAAAGAGTTGGCTACGATGCTCGCTTCGGTGAATGA
ACTCGGTATCGACAATAAGTTGACCTTTACCCGTATCGACTGGGGTGAAAAGCAATCAGAGCATTTCCTCTATCGTCTTC
CTTTAAACATTGAGTTAACGGGAGATTTTCATGACATCGGACATTTTTCTCAGGCGATAGCAACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAAAGTGAAACTCTGCACTTTAGGGTACGAGCCAATACCTATCA
GTTTAAGCCAGAGGAGACGAACAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

88.947

96.447

0.858
  pilO Vibrio cholerae strain A1552

70.051

100

0.701

Multiple sequence alignment