Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACF6ZU_RS08200 Genome accession   NZ_CP171224
Coordinates   1773180..1774397 (+) Length   405 a.a.
NCBI ID   WP_392891433.1    Uniprot ID   -
Organism   Pseudomonas migulae strain HY-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1768180..1779397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF6ZU_RS08180 (ACF6ZU_08180) - 1769600..1769896 (+) 297 WP_392893747.1 DUF2845 domain-containing protein -
  ACF6ZU_RS08185 (ACF6ZU_08185) - 1770179..1770532 (-) 354 WP_392891427.1 BON domain-containing protein -
  ACF6ZU_RS08190 (ACF6ZU_08190) - 1770841..1771251 (-) 411 WP_392891429.1 pilin -
  ACF6ZU_RS08195 (ACF6ZU_08195) pilB 1771477..1773177 (+) 1701 WP_392891431.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF6ZU_RS08200 (ACF6ZU_08200) pilC 1773180..1774397 (+) 1218 WP_392891433.1 type II secretion system F family protein Machinery gene
  ACF6ZU_RS08205 (ACF6ZU_08205) pilD 1774399..1775271 (+) 873 WP_392891434.1 prepilin peptidase Machinery gene
  ACF6ZU_RS08210 (ACF6ZU_08210) coaE 1775268..1775891 (+) 624 WP_392891435.1 dephospho-CoA kinase -
  ACF6ZU_RS08215 (ACF6ZU_08215) yacG 1775888..1776088 (+) 201 WP_392891436.1 DNA gyrase inhibitor YacG -
  ACF6ZU_RS08220 (ACF6ZU_08220) - 1776098..1776313 (-) 216 WP_008033212.1 hypothetical protein -
  ACF6ZU_RS08225 (ACF6ZU_08225) - 1776377..1777066 (-) 690 WP_392891439.1 energy-coupling factor ABC transporter permease -
  ACF6ZU_RS08230 (ACF6ZU_08230) - 1777394..1778020 (+) 627 WP_392891440.1 DUF1780 domain-containing protein -
  ACF6ZU_RS08235 (ACF6ZU_08235) - 1778017..1778532 (+) 516 WP_392891442.1 MOSC domain-containing protein -
  ACF6ZU_RS08240 (ACF6ZU_08240) - 1778601..1778774 (+) 174 WP_008028730.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44329.21 Da        Isoelectric Point: 10.0746

>NTDB_id=1060534 ACF6ZU_RS08200 WP_392891433.1 1773180..1774397(+) (pilC) [Pseudomonas migulae strain HY-2]
MAVKAAKISVYAWEGTDRKGTKMTGELSGQNPALIKAQLRKQGINPGKVRKKTASILSFGQRIKAQDIALFTRQMATMMR
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPMAVVFVAIIVTGILLIKVVPQFESVFKGFGAELPAFTVMVIGLSKFLQEWWWLILGALIAAIFGVR
RAFKRSQGFRDWTDTWLLKLPLVGTLMYKSSVARYARTLSTTFAAGVPLVEALESVAGATGNVVFKRAVLRIKQDVSTGM
QLNFSMRSSGIFPNMAIQMTAIGEESGALDDMLDKVASFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1060534 ACF6ZU_RS08200 WP_392891433.1 1773180..1774397(+) (pilC) [Pseudomonas migulae strain HY-2]
ATGGCGGTCAAAGCAGCAAAAATCAGCGTGTATGCCTGGGAAGGCACCGACAGGAAAGGCACGAAAATGACCGGCGAGCT
CAGCGGTCAGAACCCGGCGTTGATCAAGGCGCAATTGCGCAAACAAGGCATCAACCCCGGCAAGGTGCGCAAAAAAACCG
CCTCGATTCTCAGCTTCGGTCAACGCATCAAGGCCCAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAGA
GCTGGCGTGCCCTTGTTGCAGTCGTTCGACATCATCGGCGAAGGCTTCGATAACCCGGCCATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCGGCGGGTAACAGCTTCGCCGCCGCCCTGCGCAAAAAACCGCAGTATTTTGATGAGCTCTATT
GCAACCTGGTGGACGCCGGCGAACAGGCCGGTGCGTTGGACACGCTGCTGGAGCGGGTCGCGACCTACAAGGAGAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCGATGGCCGTGGTGTTTGTCGCGATCATCGTCACCGGCAT
TCTGCTGATCAAGGTCGTGCCGCAGTTCGAATCGGTGTTCAAGGGGTTCGGTGCCGAGTTGCCGGCGTTCACCGTGATGG
TCATCGGCCTGTCGAAATTCCTGCAGGAGTGGTGGTGGCTGATTCTCGGCGCGCTGATTGCGGCGATCTTCGGTGTGCGT
CGCGCCTTCAAGAGATCCCAGGGTTTCCGCGACTGGACGGACACCTGGCTGCTGAAACTGCCGCTGGTTGGCACCTTGAT
GTACAAGTCTTCCGTGGCCCGTTACGCCCGCACGCTGTCGACCACCTTCGCCGCCGGCGTGCCACTGGTGGAAGCGCTTG
AATCGGTGGCCGGCGCGACCGGTAACGTTGTGTTCAAGCGTGCGGTACTGCGCATCAAGCAGGATGTGTCGACGGGGATG
CAGCTCAATTTCTCCATGCGCAGCTCCGGCATTTTCCCGAACATGGCGATCCAGATGACGGCCATCGGCGAAGAGTCTGG
CGCGCTGGACGACATGCTCGACAAGGTCGCGAGTTTCTATGAGGCCGAGGTCGACAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGTGTTATCGTCGGCGGCCTGGTGGTTGCGATGTACCTGCCCATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baumannii D1279779

59.804

100

0.602
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilC Vibrio cholerae strain A1552

43.21

100

0.432
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

40.64

100

0.407
  pilC Thermus thermophilus HB27

37.905

99.012

0.375

Multiple sequence alignment