Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACF8MK_RS14185 Genome accession   NZ_CP171154
Coordinates   3260945..3262165 (+) Length   406 a.a.
NCBI ID   WP_265704775.1    Uniprot ID   -
Organism   Legionella sp. 227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3255945..3267165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF8MK_RS14155 (ACF8MK_14155) - 3255997..3256188 (-) 192 WP_392537199.1 DUF6356 family protein -
  ACF8MK_RS14160 (ACF8MK_14160) - 3256332..3256892 (-) 561 WP_265704782.1 hypoxanthine-guanine phosphoribosyltransferase -
  ACF8MK_RS14165 (ACF8MK_14165) - 3256939..3257265 (-) 327 WP_265704781.1 DUF1820 family protein -
  ACF8MK_RS14170 (ACF8MK_14170) - 3257421..3258002 (+) 582 WP_392537200.1 hypothetical protein -
  ACF8MK_RS14175 (ACF8MK_14175) - 3258123..3258905 (-) 783 WP_392537201.1 methyltransferase domain-containing protein -
  ACF8MK_RS14180 (ACF8MK_14180) pilB 3259212..3260933 (+) 1722 WP_265704776.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF8MK_RS14185 (ACF8MK_14185) pilC 3260945..3262165 (+) 1221 WP_265704775.1 type II secretion system F family protein Machinery gene
  ACF8MK_RS14190 (ACF8MK_14190) pilD 3262397..3263257 (+) 861 WP_392537202.1 prepilin peptidase Machinery gene
  ACF8MK_RS14195 (ACF8MK_14195) - 3263368..3264138 (+) 771 WP_392537203.1 HAD family hydrolase -
  ACF8MK_RS14200 (ACF8MK_14200) - 3264154..3265356 (+) 1203 WP_392537204.1 sterol desaturase family protein -
  ACF8MK_RS14205 (ACF8MK_14205) - 3265553..3266002 (+) 450 WP_392537205.1 CBS domain-containing protein -
  ACF8MK_RS14210 (ACF8MK_14210) - 3266046..3266600 (+) 555 WP_392537206.1 HPF/RaiA family ribosome-associated protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44408.31 Da        Isoelectric Point: 9.8833

>NTDB_id=1060346 ACF8MK_RS14185 WP_265704775.1 3260945..3262165(+) (pilC) [Legionella sp. 227]
MKKNANTTLTFHYTGVNKAAQKINGDIDARSLALAKVELRRQGIIVSKIAKKRAPFFNFKSKKIKSGDITVFSRQLATMI
ESGIPLVQSFDIVAKGQSNKRLKELIENIKHDVETGLTLSEALMKHPAHFNELFCNLVEAGEKSGSLDIMLDKVATYKEK
IETIKKKIKKALTYPMAVMVVALIVTTGLLIFVVPQFESLFKGFGADLPAMTQAVVSMSKFFQSFWYLIFGALGGGIYAF
IYAEKHSPQFAQNVDRTLLKLPVIGPIIEKAAIARFSRTLSITFAAGLPLVEALKSVAGATGNIIFAKATDQIREEVSTG
QQMNKAMENTQLFPNMVIQMVAIGEESGALEKMLSKVADFYEEDVDNAVDSLSSLLEPVIMVVLGVLVGGLVVAMYLPIF
KLGSAI

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1060346 ACF8MK_RS14185 WP_265704775.1 3260945..3262165(+) (pilC) [Legionella sp. 227]
ATGAAAAAAAATGCCAATACTACTTTAACTTTTCATTACACAGGCGTTAATAAAGCGGCTCAAAAAATAAATGGCGATAT
CGACGCCAGAAGTCTTGCTTTAGCTAAAGTAGAATTACGCAGACAAGGAATTATTGTAAGTAAAATTGCTAAAAAACGCG
CTCCTTTTTTCAATTTCAAAAGCAAAAAAATCAAGTCGGGTGATATTACGGTATTTAGTCGCCAACTGGCTACGATGATA
GAGTCTGGGATCCCTTTAGTGCAATCTTTTGATATTGTTGCAAAAGGACAGTCAAATAAACGGCTTAAGGAATTAATTGA
AAATATTAAACACGATGTTGAAACAGGATTAACCCTCTCAGAAGCATTGATGAAACATCCTGCTCATTTTAACGAATTAT
TTTGCAATTTGGTGGAAGCCGGAGAAAAATCAGGTTCTCTTGATATCATGCTCGATAAAGTCGCCACATATAAGGAAAAA
ATAGAAACAATCAAGAAGAAAATTAAAAAAGCCCTGACTTATCCTATGGCTGTTATGGTTGTCGCTCTGATTGTTACGAC
GGGATTACTTATTTTTGTTGTTCCTCAATTTGAATCATTATTTAAAGGTTTTGGTGCTGATCTACCAGCTATGACCCAAG
CAGTAGTGAGCATGTCCAAATTTTTTCAATCCTTTTGGTATTTAATTTTTGGCGCACTGGGTGGGGGGATTTACGCGTTT
ATTTATGCAGAAAAGCACTCCCCTCAATTTGCACAAAATGTGGATAGAACTTTATTAAAGTTACCCGTTATTGGTCCCAT
TATTGAGAAAGCTGCAATCGCTCGATTTTCAAGAACACTATCGATTACTTTTGCTGCAGGTTTGCCTTTAGTTGAGGCAC
TTAAATCGGTGGCAGGAGCGACAGGAAATATTATTTTCGCCAAAGCCACTGATCAAATTAGAGAAGAGGTATCAACGGGG
CAACAAATGAATAAAGCCATGGAAAATACTCAATTATTCCCCAACATGGTAATCCAGATGGTAGCCATTGGAGAAGAGTC
AGGTGCTTTAGAAAAAATGTTAAGTAAGGTTGCTGATTTTTATGAAGAAGATGTGGATAATGCGGTCGATTCACTGAGTA
GTTTATTAGAACCTGTTATTATGGTTGTTTTAGGTGTTTTGGTAGGTGGACTTGTTGTTGCAATGTATTTACCCATTTTC
AAACTGGGATCTGCAATATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

80.542

100

0.805
  pilC Acinetobacter baumannii D1279779

55.419

100

0.554
  pilC Acinetobacter baylyi ADP1

53.086

99.754

0.53
  pilC Pseudomonas stutzeri DSM 10701

51.97

100

0.52
  pilG Neisseria meningitidis 44/76-A

44.552

100

0.453
  pilG Neisseria gonorrhoeae MS11

44.552

100

0.453
  pilC Vibrio cholerae strain A1552

43.182

97.537

0.421
  pilC Vibrio campbellii strain DS40M4

39.756

100

0.401
  pilC Thermus thermophilus HB27

36.691

100

0.377

Multiple sequence alignment