Detailed information NTG1060347

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACF8MK_RS14190
• Genome accession: NZ_CP171154
• Coordinates: 3262397..3263257 (+)
• Length: 286 a.a.
• NCBI ID: WP_392537202.1
• Uniprot ID: -
• Organism: Legionella sp. 227
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3257397..3268257

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF8MK_RS14170 (ACF8MK_14170)	-	3257421..3258002 (+)	582	WP_392537200.1	hypothetical protein	-
ACF8MK_RS14175 (ACF8MK_14175)	-	3258123..3258905 (-)	783	WP_392537201.1	methyltransferase domain-containing protein	-
ACF8MK_RS14180 (ACF8MK_14180)	pilB	3259212..3260933 (+)	1722	WP_265704776.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACF8MK_RS14185 (ACF8MK_14185)	pilC	3260945..3262165 (+)	1221	WP_265704775.1	type II secretion system F family protein	Machinery gene
ACF8MK_RS14190 (ACF8MK_14190)	pilD	3262397..3263257 (+)	861	WP_392537202.1	prepilin peptidase	Machinery gene
ACF8MK_RS14195 (ACF8MK_14195)	-	3263368..3264138 (+)	771	WP_392537203.1	HAD family hydrolase	-
ACF8MK_RS14200 (ACF8MK_14200)	-	3264154..3265356 (+)	1203	WP_392537204.1	sterol desaturase family protein	-
ACF8MK_RS14205 (ACF8MK_14205)	-	3265553..3266002 (+)	450	WP_392537205.1	CBS domain-containing protein	-
ACF8MK_RS14210 (ACF8MK_14210)	-	3266046..3266600 (+)	555	WP_392537206.1	HPF/RaiA family ribosome-associated protein	-
ACF8MK_RS14215 (ACF8MK_14215)	-	3266672..3267412 (-)	741	WP_392537207.1	SDR family NAD(P)-dependent oxidoreductase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32779.23 Da
• Isoelectric Point: 8.1416

>NTDB_id=1060347 ACF8MK_RS14190 WP_392537202.1 3262397..3263257(+) (pilD) [Legionella sp. 227]
MIYDLIINHTWFMYVVVALFSLAVGSLLNVIIYRLPIMLEREWKQQYNELAGIKEEEQKPINLFFPRSFCPSCKTMVKAW
QNIPVLSYLFLRGRCYQCKSPISVRYPLIELGTMVLSLYASWHFGFTLQLIFVLAAIWILICLFFIDLDYQILPDSLTLS
LLWIGLIANTENLFAPLPQAVLSAAGGYIGLWLFIKIFYLCTGKIGMGNGDFKLFAAFGAWFGWVFLPLILLLSSISGTI
IGLLYLHLQNKSKDTTIPFGPFLCISGLISLFWGHSIVNWYLHLWM

Nucleotide

• Length: 861 bp

>NTDB_id=1060347 ACF8MK_RS14190 WP_392537202.1 3262397..3263257(+) (pilD) [Legionella sp. 227]
ATGATATATGACCTAATCATCAATCATACTTGGTTTATGTATGTCGTAGTTGCCCTTTTTTCCCTGGCTGTGGGCAGTTT
ACTCAATGTCATCATTTATCGCCTGCCTATTATGTTGGAAAGAGAATGGAAGCAACAATATAATGAGCTAGCGGGTATCA
AGGAAGAGGAACAAAAACCGATTAATTTATTTTTTCCAAGATCATTTTGCCCTTCATGTAAAACCATGGTTAAGGCCTGG
CAAAATATCCCTGTTTTAAGTTATTTATTTTTGCGTGGGCGCTGTTATCAATGTAAAAGTCCTATTTCTGTTCGTTATCC
TTTAATCGAACTAGGCACAATGGTTCTTTCCCTATACGCGAGCTGGCATTTTGGCTTTACGTTACAACTCATTTTTGTAT
TGGCTGCGATTTGGATCTTAATTTGCTTATTTTTCATTGACTTAGATTATCAAATTTTACCAGACAGCCTCACGTTGAGC
CTGTTATGGATAGGACTTATTGCCAATACGGAAAATCTATTTGCGCCCCTGCCTCAAGCAGTGCTCAGTGCAGCTGGAGG
TTATATAGGACTATGGTTATTTATAAAAATATTTTATTTATGTACTGGTAAGATTGGCATGGGCAATGGCGATTTTAAAT
TGTTTGCTGCTTTTGGAGCCTGGTTTGGTTGGGTATTTTTGCCGTTAATTCTTCTTCTTTCCTCAATAAGTGGTACAATT
ATTGGTTTATTGTATTTGCATTTGCAAAATAAATCCAAAGATACCACGATTCCTTTTGGTCCTTTTTTATGTATCAGTGG
ATTAATTTCTTTATTTTGGGGGCATAGTATCGTGAACTGGTATTTGCATCTTTGGATGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-124)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.097	100	0.486
pilD	Neisseria gonorrhoeae MS11	45.683	97.203	0.444
pilD	Vibrio campbellii strain DS40M4	45.652	96.503	0.441
pilD	Acinetobacter baumannii D1279779	44.964	97.203	0.437
pilD	Acinetobacter nosocomialis M2	44.604	97.203	0.434