Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACF8MK_RS14180 Genome accession   NZ_CP171154
Coordinates   3259212..3260933 (+) Length   573 a.a.
NCBI ID   WP_265704776.1    Uniprot ID   -
Organism   Legionella sp. 227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3254212..3265933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF8MK_RS14150 (ACF8MK_14150) - 3254829..3256010 (-) 1182 WP_392537198.1 hypothetical protein -
  ACF8MK_RS14155 (ACF8MK_14155) - 3255997..3256188 (-) 192 WP_392537199.1 DUF6356 family protein -
  ACF8MK_RS14160 (ACF8MK_14160) - 3256332..3256892 (-) 561 WP_265704782.1 hypoxanthine-guanine phosphoribosyltransferase -
  ACF8MK_RS14165 (ACF8MK_14165) - 3256939..3257265 (-) 327 WP_265704781.1 DUF1820 family protein -
  ACF8MK_RS14170 (ACF8MK_14170) - 3257421..3258002 (+) 582 WP_392537200.1 hypothetical protein -
  ACF8MK_RS14175 (ACF8MK_14175) - 3258123..3258905 (-) 783 WP_392537201.1 methyltransferase domain-containing protein -
  ACF8MK_RS14180 (ACF8MK_14180) pilB 3259212..3260933 (+) 1722 WP_265704776.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF8MK_RS14185 (ACF8MK_14185) pilC 3260945..3262165 (+) 1221 WP_265704775.1 type II secretion system F family protein Machinery gene
  ACF8MK_RS14190 (ACF8MK_14190) pilD 3262397..3263257 (+) 861 WP_392537202.1 prepilin peptidase Machinery gene
  ACF8MK_RS14195 (ACF8MK_14195) - 3263368..3264138 (+) 771 WP_392537203.1 HAD family hydrolase -
  ACF8MK_RS14200 (ACF8MK_14200) - 3264154..3265356 (+) 1203 WP_392537204.1 sterol desaturase family protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63008.56 Da        Isoelectric Point: 5.8277

>NTDB_id=1060345 ACF8MK_RS14180 WP_265704776.1 3259212..3260933(+) (pilB) [Legionella sp. 227]
MLGTDELKLHGIGQLFVLEKLLDRAQAVEFCKQAAAEKISLVQYLVKNNILSATQIALAASRNFGVPILDLDCIEVDSIP
VNLVNEKLIRRHSMVPLFYRGNNLYLATEDPSIQASLKEIQFHTGLNTHAIVVEADKLSALIDHLLTLQETQGLSEFVGG
ESDEFDGLIINEEEEHETNIATSLTEDAPIVKFVNKILLDAIKQGASDIHFEPYEKEYRIRYRQDGILHEIATPPGSLAT
RITARIKIMSNLDISERRIPQDGGFKMKISKTRSIDFRVSTCPTTSGEKVVMRILDPGSAKLGIEALGFSPNQKEHFVRS
IERPQGMILVTGPTGSGKTVTLYTALNILNTKEVNISTAEDPVEIKVPGINQVNINPKAGLTFSNALRSFLRQDPDIIMV
GEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIATFNVASSVTLIIAQRLARKLCEHCKVLRDDFNKPGL
LELGFTEADLDGIKLYKAGGCPKCTNGYRGRIGLFEVLPMTRTLGQVIMSGGNSLDILKIAQEEGMITIYQSGLEKIKQG
ITTIEEVNRVTVD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1060345 ACF8MK_RS14180 WP_265704776.1 3259212..3260933(+) (pilB) [Legionella sp. 227]
ATGTTAGGAACGGATGAATTAAAATTACATGGAATAGGACAGCTATTTGTTCTTGAAAAGTTGTTAGATAGAGCTCAAGC
TGTCGAATTCTGCAAACAAGCAGCTGCAGAAAAAATATCTTTGGTTCAATATCTTGTAAAAAATAACATTTTATCAGCCA
CACAAATTGCACTTGCAGCGTCGCGTAATTTTGGCGTTCCCATTCTAGATCTTGACTGCATTGAAGTCGATTCTATCCCC
GTGAATCTGGTTAATGAGAAATTAATTCGTCGACATTCAATGGTTCCCTTATTTTACCGAGGCAATAATTTATACCTGGC
AACAGAGGATCCAAGTATACAAGCATCTTTAAAAGAAATTCAATTCCATACTGGGCTTAATACTCATGCAATTGTCGTAG
AGGCTGATAAGCTGAGCGCGCTAATTGATCACTTACTCACATTACAAGAAACTCAGGGTTTATCCGAGTTTGTTGGTGGG
GAAAGTGATGAATTTGATGGGCTTATCATTAATGAGGAAGAAGAGCACGAAACAAATATAGCAACTTCACTCACCGAAGA
TGCTCCCATTGTAAAATTCGTCAATAAAATACTTCTTGATGCCATTAAGCAAGGAGCATCCGACATACATTTTGAACCCT
ACGAGAAAGAATATCGCATACGCTATCGACAAGATGGTATTTTACACGAAATTGCCACACCGCCAGGAAGTTTGGCTACG
CGAATTACGGCTCGCATAAAAATCATGTCCAACTTAGATATTTCAGAAAGGCGAATACCCCAGGATGGGGGATTTAAAAT
GAAAATATCCAAAACCCGATCCATCGATTTTCGCGTAAGCACCTGCCCTACTACATCAGGTGAAAAAGTTGTTATGCGTA
TCTTAGATCCTGGTTCAGCAAAACTTGGTATTGAGGCTTTAGGTTTTAGCCCGAACCAAAAAGAACATTTTGTCCGTTCT
ATTGAACGACCTCAAGGTATGATTCTAGTGACAGGTCCCACCGGTAGTGGTAAAACGGTAACCTTGTATACCGCATTGAA
TATACTTAATACTAAAGAAGTTAATATCTCTACAGCTGAAGACCCGGTTGAAATTAAAGTGCCAGGGATCAATCAGGTTA
ATATTAATCCCAAAGCAGGTTTAACCTTTTCCAACGCGTTACGATCGTTTTTACGTCAAGACCCTGACATCATCATGGTT
GGCGAGATACGTGATTTGGAAACCGCGGAAATAGCAGTTAAGGCAGCGCAAACAGGACACTTAGTACTCTCAACATTGCA
TACAAACAGTGCAGCTGAAACACTGAACCGTTTGGTGAATATGGGGATTGCAACATTTAATGTTGCCAGTTCAGTAACAC
TTATTATTGCACAACGTTTAGCTCGAAAATTATGTGAGCATTGTAAAGTATTGAGGGATGACTTCAATAAGCCAGGCTTG
CTGGAATTAGGTTTTACTGAAGCAGATTTAGATGGAATTAAATTATATAAAGCTGGAGGATGTCCTAAATGTACCAATGG
ATACCGCGGTAGAATAGGTTTATTTGAAGTATTGCCTATGACCAGAACACTTGGCCAGGTAATCATGTCTGGAGGAAATT
CCTTAGATATATTAAAGATTGCTCAGGAAGAAGGCATGATTACTATTTATCAATCTGGGCTTGAGAAGATAAAACAAGGT
ATTACAACTATAGAGGAAGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

80.279

100

0.803
  pilB Acinetobacter baylyi ADP1

50.521

100

0.508
  pilB Acinetobacter baumannii D1279779

50.883

98.778

0.503
  pilB Vibrio campbellii strain DS40M4

48.463

96.51

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

48.288

96.859

0.468
  pilB Vibrio cholerae strain A1552

48.282

96.51

0.466
  pilF Neisseria gonorrhoeae MS11

46.996

98.778

0.464
  pilF Thermus thermophilus HB27

40.968

93.717

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.811

92.496

0.368

Multiple sequence alignment