Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACFX5Z_RS19660 Genome accession   NZ_CP170761
Coordinates   4287698..4288939 (+) Length   413 a.a.
NCBI ID   WP_010635960.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain CWH5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4282698..4293939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFX5Z_RS19635 (ACFX5Z_19635) ampD 4283086..4283658 (-) 573 WP_279452167.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACFX5Z_RS19640 (ACFX5Z_19640) - 4283782..4284252 (+) 471 WP_010635963.1 TIGR02281 family clan AA aspartic protease -
  ACFX5Z_RS19645 (ACFX5Z_19645) nadC 4284257..4285114 (+) 858 WP_010635962.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFX5Z_RS19650 (ACFX5Z_19650) - 4285436..4285876 (+) 441 WP_010635961.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACFX5Z_RS19655 (ACFX5Z_19655) pilB 4285867..4287582 (+) 1716 WP_185450531.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACFX5Z_RS19660 (ACFX5Z_19660) pilC 4287698..4288939 (+) 1242 WP_010635960.1 type II secretion system F family protein Machinery gene
  ACFX5Z_RS19665 (ACFX5Z_19665) pilD 4288962..4289834 (+) 873 WP_201889602.1 A24 family peptidase Machinery gene
  ACFX5Z_RS19670 (ACFX5Z_19670) coaE 4289854..4290468 (+) 615 WP_126880609.1 dephospho-CoA kinase -
  ACFX5Z_RS19675 (ACFX5Z_19675) zapD 4290506..4291228 (+) 723 WP_026141277.1 cell division protein ZapD -
  ACFX5Z_RS19680 (ACFX5Z_19680) yacG 4291238..4291432 (+) 195 WP_017782442.1 DNA gyrase inhibitor YacG -
  ACFX5Z_RS19685 (ACFX5Z_19685) mutT 4291493..4291906 (-) 414 WP_005305665.1 8-oxo-dGTP diphosphatase MutT -
  ACFX5Z_RS19690 (ACFX5Z_19690) - 4291915..4293093 (-) 1179 WP_279452164.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45468.58 Da        Isoelectric Point: 9.8834

>NTDB_id=1059317 ACFX5Z_RS19660 WP_010635960.1 4287698..4288939(+) (pilC) [Aeromonas dhakensis strain CWH5]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGARIKPMDIAVVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPTMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPAFTQFVIGISRFMQNWWYMIFGGVA
LAIFLYVRAWRASQKVRDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALISAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=1059317 ACFX5Z_RS19660 WP_010635960.1 4287698..4288939(+) (pilC) [Aeromonas dhakensis strain CWH5]
ATGGCAACGCTAACCCAGAAACAGAATGCCCCCAAGAAAGTCTTCGCCTTTCGCTGGAGCGGGGTGAACCGCAAGGGCCA
GAAGGTCTCCGGCGAGCTGCAGGCCGACAGCATCAACACGGTCAAGGCCGAGCTGCGCAAGCAGGGCGTCAACGTCACCA
AGGTGAGCAAGAAGAGTCAGGGGCTCTTCTCCAAGGGCGGCGCCAGGATCAAGCCGATGGACATTGCCGTCGTCTCTCGC
CAGATCACAACCATGCTGTCGGCCGGCGTACCTCTGGTCCAGAGCCTGCAGATCATCGCTCGCAGCCACGAGAAGGCGGC
CATGCGCGAGCTGATGGGCCAGATAGCCGCCGATGTGGAGACCGGCACCCCCATGTCCGAGGCGCTGCGCCGCCATCCCC
GCCACTTCGATGATCTCTATTGCGACCTGGTCGAGGCGGGCGAGCAGTCCGGCGCCCTGGAGACCATCTACGACCGCATC
GCGACTTATCGCGAAAAGTCCGAAGCGCTCAAATCCAAGATCAAGAAGGCCATGTTCTACCCCACCATGGTCATCCTGGT
GGCCATTGTCGTCACCTCCATTCTGCTGCTGTTCGTCATTCCGCAGTTTGAGGACATCTTCAAGAGCTTCGGCGCCGAAC
TGCCCGCCTTTACCCAGTTCGTCATCGGCATATCCCGCTTCATGCAGAACTGGTGGTATATGATCTTTGGCGGGGTTGCA
CTCGCCATCTTCCTCTACGTGCGAGCCTGGCGTGCCTCGCAGAAAGTCAGAGACAACACCGACAAGTTCATTCTGACTAT
CCCGGTGGTCGGCATGATATTGCACAAGGCCGCCATGGCCCGCTTCGCCCGCACCCTCTCCACCACCTTTTCTGCCGGTA
TCCCGCTGGTGGATGCCCTGATCTCCGCCGCTGGTGCCTCCGGCAACTATGTCTATCGCACCGCCGTCATGGCCATTCGT
AACGAGGTGGTGGCTGGCATGCAGATCAACGTGGCCATGCGCACCGTCGATCTCTTCCCCGACATGGTGATTCAGATGGT
GATGATTGGCGAGGAGTCAGGCGCCATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAACAGGAGGTGGACGATC
TAGTCGACGGCCTCACCAGCCTGCTGGAACCTCTCATCATGGTGGTGCTGGGGGTACTGGTCGGCGGCATGGTGGTCGCC
ATGTACCTGCCCATCTTCAAACTGGGGTCGGTCATCCACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.323

95.884

0.55
  pilC Acinetobacter baumannii D1279779

54

96.852

0.523
  pilC Legionella pneumophila strain ERS1305867

51.358

98.063

0.504
  pilC Acinetobacter baylyi ADP1

52.141

96.126

0.501
  pilC Vibrio cholerae strain A1552

48.241

96.368

0.465
  pilC Vibrio campbellii strain DS40M4

47.607

96.126

0.458
  pilG Neisseria meningitidis 44/76-A

40.247

98.063

0.395
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilC Thermus thermophilus HB27

38

96.852

0.368

Multiple sequence alignment