Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFX5Z_RS19655 Genome accession   NZ_CP170761
Coordinates   4285867..4287582 (+) Length   571 a.a.
NCBI ID   WP_185450531.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain CWH5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4280867..4292582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFX5Z_RS19630 (ACFX5Z_19630) pdhR 4282046..4282810 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACFX5Z_RS19635 (ACFX5Z_19635) ampD 4283086..4283658 (-) 573 WP_279452167.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACFX5Z_RS19640 (ACFX5Z_19640) - 4283782..4284252 (+) 471 WP_010635963.1 TIGR02281 family clan AA aspartic protease -
  ACFX5Z_RS19645 (ACFX5Z_19645) nadC 4284257..4285114 (+) 858 WP_010635962.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFX5Z_RS19650 (ACFX5Z_19650) - 4285436..4285876 (+) 441 WP_010635961.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACFX5Z_RS19655 (ACFX5Z_19655) pilB 4285867..4287582 (+) 1716 WP_185450531.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACFX5Z_RS19660 (ACFX5Z_19660) pilC 4287698..4288939 (+) 1242 WP_010635960.1 type II secretion system F family protein Machinery gene
  ACFX5Z_RS19665 (ACFX5Z_19665) pilD 4288962..4289834 (+) 873 WP_201889602.1 A24 family peptidase Machinery gene
  ACFX5Z_RS19670 (ACFX5Z_19670) coaE 4289854..4290468 (+) 615 WP_126880609.1 dephospho-CoA kinase -
  ACFX5Z_RS19675 (ACFX5Z_19675) zapD 4290506..4291228 (+) 723 WP_026141277.1 cell division protein ZapD -
  ACFX5Z_RS19680 (ACFX5Z_19680) yacG 4291238..4291432 (+) 195 WP_017782442.1 DNA gyrase inhibitor YacG -
  ACFX5Z_RS19685 (ACFX5Z_19685) mutT 4291493..4291906 (-) 414 WP_005305665.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62871.40 Da        Isoelectric Point: 5.4363

>NTDB_id=1059316 ACFX5Z_RS19655 WP_185450531.1 4285867..4287582(+) (pilB) [Aeromonas dhakensis strain CWH5]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMESDQDALGMEEIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1059316 ACFX5Z_RS19655 WP_185450531.1 4285867..4287582(+) (pilB) [Aeromonas dhakensis strain CWH5]
CTGCCCATAATGACATCTAGTCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAGGCCAAGGCGCAACGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGAAATCCTCGACAGCA
AGGCGCTGGCCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGC
CATGTCCGACCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTGCACACCGAGGCCTTGCTGGTCG
AGGAGAACAAGCTGACCGCCGCCATCAGCAAGCTGATGGAGAGCGATCAGGACGCCCTTGGCATGGAGGAGATCGACGAG
TCCGAGATTTCCGAGCTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCC
CATCGTCAAATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGA
CCAAGTACCGTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCAACGCCGCCGGTCAATCTGGCCAACCGCTTC
TCGGCCCGACTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAAAT
GTCGCGCAACAAGTCGATGGACATGCGGGTCAACACCCTGCCTACCATGTGGGGCGAGAAGATCGTGATCCGTCTGCTCG
ACTCTTCCGCTGCGCGCCTCAATATCGAGCAGCTCGGCTTCGACGATCGGCAGAAGGCGCAATATCTGCGTGCGCTCTCG
AAGCCTCAGGGCATGATCCTGGTGACCGGTCCGACCGGCTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATCCT
CAACACCACAGAGGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCAGGAGTCAATCAGGTGCAGGTCA
ACCCCAAGGCAGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACGTGGTGATGGTCGGGGAA
ATACGGGATCTAGAAACCGCCGAGATCGCCATCAAGGCGGCACAAACCGGTCACCTGGTGCTTTCCACATTGCACACCAA
CTCGGCAGCCGAGACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCA
TGGCTCAGCGACTGGCACGCAAGCTCTGTGACCACTGCAAGGCTCCCGAGGTGGTGCCGGAGGCTGAACTGCTGGAGTTG
GGTTTTACCCAACAGCAGCTGGCAGCAGGCTTGCGGCTGTTCAAGCCGGTTGGCTGCAAGGAGTGTTCTGGCGGCTACAA
GGGACGGGTCGGCATCTACGAGATCCTGTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAACTCCATGC
AGATTGCGGCCATTGCCCAGAAGGAGGGCATGCGGACCTTGCGTACCTCCGGTCTTGAGAAGGCCCGACTGGGGGTGACC
AGCCTGGCCGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Acinetobacter baumannii D1279779

54.783

100

0.552
  pilB Legionella pneumophila strain ERS1305867

54.594

99.124

0.541
  pilB Vibrio cholerae strain A1552

54.159

98.949

0.536
  pilB Vibrio campbellii strain DS40M4

51.767

99.124

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.424

0.503
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

96.848

0.375
  pilF Thermus thermophilus HB27

40.53

92.469

0.375

Multiple sequence alignment