Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACFU5E_RS02215 Genome accession   NZ_CP170577
Coordinates   480895..482136 (-) Length   413 a.a.
NCBI ID   WP_098981882.1    Uniprot ID   -
Organism   Aeromonas bestiarum strain S1-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 475895..487136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFU5E_RS02185 (ACFU5E_02185) - 476673..477857 (+) 1185 WP_378081156.1 tetratricopeptide repeat protein -
  ACFU5E_RS02190 (ACFU5E_02190) mutT 477866..478273 (+) 408 WP_098981871.1 8-oxo-dGTP diphosphatase MutT -
  ACFU5E_RS02195 (ACFU5E_02195) yacG 478363..478557 (-) 195 WP_043139929.1 DNA gyrase inhibitor YacG -
  ACFU5E_RS02200 (ACFU5E_02200) zapD 478567..479289 (-) 723 WP_378081157.1 cell division protein ZapD -
  ACFU5E_RS02205 (ACFU5E_02205) coaE 479327..479941 (-) 615 WP_378081159.1 dephospho-CoA kinase -
  ACFU5E_RS02210 (ACFU5E_02210) pilD 479960..480832 (-) 873 WP_378081160.1 type IVa prepilin peptidase/methyltransferase TapD Machinery gene
  ACFU5E_RS02215 (ACFU5E_02215) pilC 480895..482136 (-) 1242 WP_098981882.1 type II secretion system F family protein Machinery gene
  ACFU5E_RS02220 (ACFU5E_02220) pilB 482269..483975 (-) 1707 WP_378081161.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACFU5E_RS02225 (ACFU5E_02225) - 483976..484389 (-) 414 WP_378081162.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACFU5E_RS02230 (ACFU5E_02230) nadC 484711..485568 (-) 858 WP_348687474.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFU5E_RS02235 (ACFU5E_02235) - 485574..486044 (-) 471 WP_378081163.1 TIGR02281 family clan AA aspartic protease -
  ACFU5E_RS02240 (ACFU5E_02240) ampD 486193..486765 (+) 573 WP_378081164.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45371.49 Da        Isoelectric Point: 9.8637

>NTDB_id=1058080 ACFU5E_RS02215 WP_098981882.1 480895..482136(-) (pilC) [Aeromonas bestiarum strain S1-4]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGAKIKPMDIAIVSR
QITTMLSAGVPLVQSLQIIARSHEKASMRELMGQIAADVETGTPMSEALRRHPRHFDALYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPAMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQFVIGISRFMQHWWYVIFGGTA
FAIFLYVRAWRASQKVKDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALISAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGDVVG

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=1058080 ACFU5E_RS02215 WP_098981882.1 480895..482136(-) (pilC) [Aeromonas bestiarum strain S1-4]
ATGGCAACGCTAACGCAGAAACAGAATGCCCCCAAGAAAGTCTTCGCCTTCCGCTGGAGCGGAGTGAACCGCAAGGGCCA
GAAGGTCTCCGGTGAATTGCAGGCCGACAGTATCAACACGGTCAAAGCTGAACTGCGCAAGCAGGGTGTCAACGTCACCA
AGGTGAGCAAGAAGAGCCAAGGGTTATTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATTGCCATCGTCTCCCGT
CAAATCACCACCATGCTCTCCGCTGGCGTCCCCCTGGTGCAGAGCCTGCAGATCATAGCCCGCAGCCACGAAAAAGCCTC
GATGCGTGAGCTCATGGGACAGATCGCCGCCGACGTGGAAACCGGCACCCCCATGTCCGAGGCGCTGCGCCGCCATCCCC
GCCACTTTGATGCGCTCTATTGTGATCTGGTTGAGGCGGGGGAGCAATCCGGTGCGCTGGAGACCATCTATGACCGCATC
GCCACCTATCGGGAAAAGTCGGAAGCGCTCAAGTCCAAGATCAAGAAGGCGATGTTCTATCCCGCCATGGTCATCCTGGT
CGCCATCGTCGTCACCTCCATACTGCTGCTGTTCGTCATCCCGCAGTTTGAGGACATCTTCAAGAGCTTCGGCGCAGAAC
TGCCCATCTTCACCCAGTTTGTCATCGGCATCTCCCGCTTCATGCAGCATTGGTGGTATGTCATCTTTGGCGGCACAGCG
TTTGCCATTTTCCTCTATGTACGGGCATGGCGCGCGTCCCAGAAGGTCAAAGACAATACCGACAAGTTCATCCTCACCAT
ACCCGTGGTCGGCATGATACTGCACAAGGCGGCCATGGCCCGCTTCGCCCGCACCCTCTCCACTACCTTCTCCGCCGGTA
TCCCACTGGTTGATGCACTGATCTCGGCCGCCGGTGCCTCCGGCAACTATGTCTATCGCACCGCCGTCATGGCCATTCGC
AACGAGGTGGTCGCCGGCATGCAGATCAACGTGGCCATGCGCACAGTGGACCTCTTCCCCGACATGGTGATCCAGATGGT
GATGATCGGCGAGGAGTCAGGCGCCATCGATGATATGCTCTCCAAGGTCGCGACCATCTTCGAACAGGAGGTTGATGACT
TGGTCGACGGCCTCACCAGCTTGCTCGAGCCCCTCATCATGGTAGTGCTGGGGGTACTGGTTGGTGGCATGGTCGTGGCC
ATGTACTTGCCCATCTTCAAGCTGGGAGACGTAGTAGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.071

95.884

0.547
  pilC Acinetobacter baumannii D1279779

54

96.852

0.523
  pilC Acinetobacter baylyi ADP1

52.141

96.126

0.501
  pilC Legionella pneumophila strain ERS1305867

51.111

98.063

0.501
  pilC Vibrio cholerae strain A1552

47.87

96.61

0.462
  pilC Vibrio campbellii strain DS40M4

46.287

97.821

0.453
  pilG Neisseria gonorrhoeae MS11

40.741

98.063

0.4
  pilG Neisseria meningitidis 44/76-A

40.494

98.063

0.397
  pilC Thermus thermophilus HB27

38

96.852

0.368

Multiple sequence alignment