Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFU5E_RS02220 Genome accession   NZ_CP170577
Coordinates   482269..483975 (-) Length   568 a.a.
NCBI ID   WP_378081161.1    Uniprot ID   -
Organism   Aeromonas bestiarum strain S1-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477269..488975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFU5E_RS02190 (ACFU5E_02190) mutT 477866..478273 (+) 408 WP_098981871.1 8-oxo-dGTP diphosphatase MutT -
  ACFU5E_RS02195 (ACFU5E_02195) yacG 478363..478557 (-) 195 WP_043139929.1 DNA gyrase inhibitor YacG -
  ACFU5E_RS02200 (ACFU5E_02200) zapD 478567..479289 (-) 723 WP_378081157.1 cell division protein ZapD -
  ACFU5E_RS02205 (ACFU5E_02205) coaE 479327..479941 (-) 615 WP_378081159.1 dephospho-CoA kinase -
  ACFU5E_RS02210 (ACFU5E_02210) pilD 479960..480832 (-) 873 WP_378081160.1 type IVa prepilin peptidase/methyltransferase TapD Machinery gene
  ACFU5E_RS02215 (ACFU5E_02215) pilC 480895..482136 (-) 1242 WP_098981882.1 type II secretion system F family protein Machinery gene
  ACFU5E_RS02220 (ACFU5E_02220) pilB 482269..483975 (-) 1707 WP_378081161.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACFU5E_RS02225 (ACFU5E_02225) - 483976..484389 (-) 414 WP_378081162.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACFU5E_RS02230 (ACFU5E_02230) nadC 484711..485568 (-) 858 WP_348687474.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFU5E_RS02235 (ACFU5E_02235) - 485574..486044 (-) 471 WP_378081163.1 TIGR02281 family clan AA aspartic protease -
  ACFU5E_RS02240 (ACFU5E_02240) ampD 486193..486765 (+) 573 WP_378081164.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACFU5E_RS02245 (ACFU5E_02245) pdhR 487042..487806 (+) 765 WP_042039018.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62576.96 Da        Isoelectric Point: 5.4321

>NTDB_id=1058081 ACFU5E_RS02220 WP_378081161.1 482269..483975(-) (pilB) [Aeromonas bestiarum strain S1-4]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSRALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLLESDQDALGMEDIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKDCSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARIGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1058081 ACFU5E_RS02220 WP_378081161.1 482269..483975(-) (pilB) [Aeromonas bestiarum strain S1-4]
ATGACCTCTAGCCCGAATAGCGGCCTGGCGTTAAGCCTGGCCGCTTCTTCCCTGCTCAGTGAGTCCGACTCACAGCGCTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAGCCCTTTGTGACCTTTCTGATAGAGAACGAGATCCTTGATAGCAGGGCGCTGG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTTGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTACTGCCCATCTACACCCAGGGCCATACCCTCTATATCGCCATGTCGGA
TCCGACCAACGTCTCGGCGCTGGAAGATTTCGGCTTCAGCTTCGGGCTGCATACCGAGGCGCTGCTGGTCGAAGAGAACA
AGCTGACGACGGCCATCGGCAAGCTGCTGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCGCGACTCGATGAGAGCGTCAACACCGCCGATGACGACGCCCCCATCGTCAA
ATACATCAATAAAATCATGATGGATGCCATCAAACGCGGCGCATCCGACCTGCACTTTGAACCCTATGAGACCAAATACC
GCATCCGCTTCCGGATCGACGGCATATTGCACGAAATCGCGACACCACCGGTCAATCTGGCCAACCGCTTCTCCGCCCGT
CTCAAGGTCATGGCGCGGCTCGACATCGCCGAACGGCGGCTGCCGCAGGATGGCCGCATCAAGCTGAAGCTATCCCGCAA
CAAGTCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAGAAGATCGTCATCCGCTTGCTGGACTCCTCAG
CGGCGCGGCTCAACATAGAGCAACTCGGCTTCGACGACCGGCAGAAGGCCCAATATCTGCGTGCCCTCTCCAAGCCGCAG
GGCATGATCTTGGTGACAGGCCCCACCGGCTCGGGCAAGACGGTCTCTCTCTATACCGGTCTCAATATTCTCAACACCAC
GGAGGTGAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCAGGCGTCAATCAGGTACAGGTCAACCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGCTCCTTTCTTCGCCAGGATCCCGATGTGGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACAGGCCATCTGGTGCTCTCCACCCTGCACACTAACTCGGCCGC
CGAAACCCTGACCCGCATGATGAACATGGGGGTGCCCGCCTTCAACATTGCCTCCTCGGTGACCTTGATCATGGCCCAGC
GACTCGCCCGCAAGCTGTGCGATCATTGCAAGGCACCCGAGGTGGTACCCGAAGCCGAGCTGCTGGAGCTGGGATTCACC
CAGCAGCAGCTGGCCGCCGGACTGCGCCTGTTCAAACCCGTGGGGTGCAAGGATTGCTCGGGCGGCTACAAAGGGCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGTGCCAACTCTCTGCAAATTGCGG
CCATTGCTCAAAAAGAGGGGATGCGCACTCTGCGTATATCCGGTCTTGAGAAGGCTCGTATTGGAGTGACCAGCCTGGCA
GAAATCAATCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.106

100

0.551
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

53.982

99.472

0.537
  pilB Vibrio campbellii strain DS40M4

51.045

100

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.277

99.296

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment