Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BCU28_RS14145 Genome accession   NZ_CP170022
Coordinates   3177696..3178925 (+) Length   409 a.a.
NCBI ID   WP_016790754.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.45.B7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3172696..3183925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU28_RS14125 (BCU28_14125) ampD 3173696..3174295 (-) 600 WP_016790757.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCU28_RS14130 (BCU28_14130) nadC 3174379..3175269 (+) 891 WP_004738163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCU28_RS14135 (BCU28_14135) pilA 3175523..3175996 (+) 474 WP_016790756.1 pilin Machinery gene
  BCU28_RS14140 (BCU28_14140) pilB 3175996..3177681 (+) 1686 WP_016790755.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCU28_RS14145 (BCU28_14145) pilC 3177696..3178925 (+) 1230 WP_016790754.1 type II secretion system F family protein Machinery gene
  BCU28_RS14150 (BCU28_14150) pilD 3178992..3179861 (+) 870 WP_016790753.1 prepilin peptidase Machinery gene
  BCU28_RS14155 (BCU28_14155) coaE 3179864..3180478 (+) 615 WP_010435920.1 dephospho-CoA kinase -
  BCU28_RS14160 (BCU28_14160) zapD 3180505..3181245 (+) 741 WP_010435917.1 cell division protein ZapD -
  BCU28_RS14165 (BCU28_14165) yacG 3181327..3181524 (+) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCU28_RS14170 (BCU28_14170) ltrA 3181803..3183086 (-) 1284 WP_016767640.1 group II intron reverse transcriptase/maturase -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 45204.14 Da        Isoelectric Point: 10.1000

>NTDB_id=1054701 BCU28_RS14145 WP_016790754.1 3177696..3178925(+) (pilC) [Vibrio cyclitrophicus strain 10N.286.45.B7]
MSSKSKQSQLKNYHWKGINSSGKKVSGQSLALTELEVREKLKEQHIQIKKIKKKSISAITRITHRVKAKDITILTRQLAT
MLATGVPIVQAIKLVSDNHRKAEMKSILSHICKGVEAGTPISKAMRTASRHFDDLYTDLVATGELSGNLAQVFERLATYR
EKSEQLKAKVVKALIYPAMVVTVALTVSYLMLTMVIPEFESMFSGFGADLPWFTQQVLDLSHWMQAYSFYTALGIGATTL
VVHQLCQRSYSIRLSASRLVLRFPVLGSVMAKASIAKFSRTLSTSFSSGIPILMSLKTTAKTAGNLHYESAIIEVHRETA
AGMPMYIAMRNTNAFPEMVLQMVMIGEESGNLDDMLNKVASIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLP
IFNLMSVLG

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1054701 BCU28_RS14145 WP_016790754.1 3177696..3178925(+) (pilC) [Vibrio cyclitrophicus strain 10N.286.45.B7]
ATGAGTAGTAAAAGCAAACAATCACAACTAAAAAACTACCATTGGAAGGGAATCAATAGTTCTGGTAAGAAAGTGTCTGG
GCAGAGCTTAGCGCTCACCGAATTAGAAGTGCGAGAGAAGCTCAAAGAGCAGCATATCCAAATTAAGAAAATCAAAAAGA
AAAGTATCTCCGCAATCACTCGCATAACACACCGAGTCAAAGCCAAGGACATCACTATTTTGACGAGACAGCTTGCTACC
ATGCTCGCTACGGGTGTGCCTATCGTGCAAGCCATAAAGTTGGTATCAGATAATCATCGTAAAGCAGAAATGAAATCGAT
TTTATCGCATATCTGCAAAGGTGTAGAAGCCGGAACGCCAATCTCAAAAGCAATGCGAACCGCAAGCCGCCACTTTGATG
ATCTCTATACTGATTTAGTCGCGACAGGAGAGCTCTCCGGCAATCTTGCACAAGTATTCGAACGCTTAGCGACATATCGT
GAAAAAAGTGAACAGTTAAAAGCTAAGGTGGTGAAAGCGCTTATCTATCCAGCAATGGTGGTCACGGTTGCGCTGACAGT
CTCCTATCTCATGCTAACCATGGTCATCCCAGAGTTTGAATCGATGTTTTCAGGCTTTGGTGCCGATCTTCCCTGGTTCA
CCCAACAAGTCCTCGACCTGTCTCATTGGATGCAAGCTTACAGTTTTTATACCGCCCTAGGCATCGGGGCTACCACGCTG
GTTGTTCATCAACTGTGCCAACGTTCTTACTCGATTCGACTATCAGCCAGCCGCTTAGTGTTACGCTTTCCTGTACTTGG
TAGCGTAATGGCAAAGGCCTCTATCGCCAAGTTCAGCCGCACCTTATCAACCAGCTTTAGCTCTGGTATTCCCATCTTAA
TGAGCCTCAAAACCACCGCAAAGACTGCAGGTAATCTGCATTATGAATCTGCGATCATCGAAGTACACCGAGAAACAGCC
GCGGGTATGCCGATGTATATTGCAATGCGCAATACCAATGCCTTCCCTGAAATGGTGTTACAGATGGTGATGATTGGTGA
AGAGTCAGGTAACCTTGATGATATGCTGAATAAAGTCGCTTCCATCTATGAATTTGAAGTCGACAATACCGTCGACAACT
TAGGCAAAATTTTAGAGCCACTGATCATCGTGTTTTTAGGTACAGTTGTGGGTGGGCTTGTTGTCGCGATGTACTTACCA
ATCTTTAATCTTATGAGTGTGTTAGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(278-400)

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

79.75

97.8

0.78
  pilC Vibrio campbellii strain DS40M4

74.138

99.267

0.736
  pilC Acinetobacter baylyi ADP1

42.054

100

0.421
  pilC Acinetobacter baumannii D1279779

41.584

98.778

0.411
  pilC Legionella pneumophila strain ERS1305867

41.814

97.066

0.406
  pilC Pseudomonas stutzeri DSM 10701

40.594

98.778

0.401
  pilG Neisseria gonorrhoeae MS11

40.351

97.555

0.394
  pilG Neisseria meningitidis 44/76-A

40.1

97.555

0.391

Multiple sequence alignment