Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCU28_RS14140 Genome accession   NZ_CP170022
Coordinates   3175996..3177681 (+) Length   561 a.a.
NCBI ID   WP_016790755.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.45.B7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3170996..3182681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU28_RS14120 (BCU28_14120) pdhR 3172453..3173214 (-) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BCU28_RS14125 (BCU28_14125) ampD 3173696..3174295 (-) 600 WP_016790757.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCU28_RS14130 (BCU28_14130) nadC 3174379..3175269 (+) 891 WP_004738163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCU28_RS14135 (BCU28_14135) pilA 3175523..3175996 (+) 474 WP_016790756.1 pilin Machinery gene
  BCU28_RS14140 (BCU28_14140) pilB 3175996..3177681 (+) 1686 WP_016790755.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCU28_RS14145 (BCU28_14145) pilC 3177696..3178925 (+) 1230 WP_016790754.1 type II secretion system F family protein Machinery gene
  BCU28_RS14150 (BCU28_14150) pilD 3178992..3179861 (+) 870 WP_016790753.1 prepilin peptidase Machinery gene
  BCU28_RS14155 (BCU28_14155) coaE 3179864..3180478 (+) 615 WP_010435920.1 dephospho-CoA kinase -
  BCU28_RS14160 (BCU28_14160) zapD 3180505..3181245 (+) 741 WP_010435917.1 cell division protein ZapD -
  BCU28_RS14165 (BCU28_14165) yacG 3181327..3181524 (+) 198 WP_010435916.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62090.84 Da        Isoelectric Point: 5.4344

>NTDB_id=1054700 BCU28_RS14140 WP_016790755.1 3175996..3177681(+) (pilB) [Vibrio cyclitrophicus strain 10N.286.45.B7]
MLTNLPTVLRQAGLLSLTQEQAVAEHVEASGISTPEALLALNFFSSDSLANNIKTIFGLPLVRLANTDYESLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQVELVVANYSELEGAIRKLYGRSISGQYSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTADDSPDIYLRHSIPNSQT
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGIDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1054700 BCU28_RS14140 WP_016790755.1 3175996..3177681(+) (pilB) [Vibrio cyclitrophicus strain 10N.286.45.B7]
ATGTTAACCAACCTCCCAACCGTTCTCCGTCAGGCTGGCTTACTTAGCCTGACTCAGGAACAAGCTGTTGCGGAACATGT
GGAAGCTTCAGGTATATCTACACCTGAAGCTTTACTCGCTCTGAATTTCTTCAGCAGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACGATTAGCCAATACTGACTACGAATCCCTGTGTGAACAATTAGGACTTCGTGAA
CTGATTACTAAGTATCGTGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAGGCTGAAGATGACTTCCGCTTTGCGACAGGATTGCAAGTTGAATTGGTTGTCGCTAACTACTCTGAATTAGAGGGTG
CAATACGAAAATTGTATGGTCGTTCTATTTCCGGGCAATACTCCAAACGCAAAGAGATCACCCAAGATGAGCTAGCTAAC
CTCGTCAAGGTATCTGACGACGAAATAACGTCGATAGAAGATCTCAGCCAAGATGACTCTCCTGTGAGTCGCTTTATCAA
TCAGATACTGGTTGATGCGGTGCGTAAAGGCGCATCGGACATCCACTTCGAACCTTATGAAGAAAACTACCGCGTACGTC
TACGTTGCGATGGCATCCTTGTCGAGATCCAACAACCCGCCTCTCATTTAAGCCGTCGTCTGTCAGCTCGCTTAAAGATC
CTCGCTAAACTCGATATTGCCGAACGTCGCTTACCTCAAGATGGCCGTATCAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACGCTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATAGACAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCAGTAGAGATTAACCTGTGTGGTATCAATCAGGTACAGGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAAATCGCGATCAAAGCCTCCCAAACCGGGCATTTAGTACTTTCAACACTGCACACTAACTCAGCCGCAGAAACCGT
GACCCGGCTTGCTCATATGGGAATCGAGCCCTTCAACCTTGCTTCGTCTTTGAGCTTGATTATTGCTCAGCGTCTTGCGA
GACGCTTGTGTAACCACTGTAAAACCGCAGACGACTCACCAGACATTTATCTGCGCCACTCTATCCCAAACAGCCAAACC
ATCTACAAAGCCAACTCTCAAGGCTGTAATGAATGTAACCAAGGTTACTCAGGTCGAGTCGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAATAAACCCAATGCGCTAGCGATTGAAGATCTCGCACGTCGAGAAGGCA
TGAGAACTTTACAAGAGTCAGGGATAGATAAACTGCTTGAAGGCACCACCAGCTACCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.936

100

0.743
  pilB Vibrio campbellii strain DS40M4

71.352

100

0.715
  pilB Vibrio parahaemolyticus RIMD 2210633

71.174

100

0.713
  pilB Legionella pneumophila strain ERS1305867

48.188

98.396

0.474
  pilB Acinetobacter baumannii D1279779

50.674

92.513

0.469
  pilB Acinetobacter baylyi ADP1

49.526

93.939

0.465
  pilF Neisseria gonorrhoeae MS11

44.643

99.822

0.446
  pilF Thermus thermophilus HB27

38.772

92.87

0.36

Multiple sequence alignment