Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BCU99_RS14145 Genome accession   NZ_CP170015
Coordinates   3125847..3127538 (-) Length   563 a.a.
NCBI ID   WP_010435279.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.52.F6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3120847..3132538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU99_RS14125 (BCU99_14125) - 3121526..3122371 (-) 846 WP_010435266.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  BCU99_RS14130 (BCU99_14130) - 3122441..3123958 (-) 1518 WP_010435269.1 SPOR domain-containing protein -
  BCU99_RS14135 (BCU99_14135) aroB 3124039..3125127 (-) 1089 WP_010435273.1 3-dehydroquinate synthase -
  BCU99_RS14140 (BCU99_14140) aroK 3125152..3125670 (-) 519 WP_009848907.1 shikimate kinase AroK -
  BCU99_RS14145 (BCU99_14145) pilQ 3125847..3127538 (-) 1692 WP_010435279.1 type IV pilus secretin PilQ Machinery gene
  BCU99_RS14150 (BCU99_14150) pilP 3127598..3128116 (-) 519 WP_010435282.1 pilus assembly protein PilP Machinery gene
  BCU99_RS14155 (BCU99_14155) pilO 3128103..3128732 (-) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  BCU99_RS14160 (BCU99_14160) pilN 3128725..3129339 (-) 615 WP_016790176.1 PilN domain-containing protein Machinery gene
  BCU99_RS14165 (BCU99_14165) pilM 3129323..3130348 (-) 1026 WP_016787692.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61434.95 Da        Isoelectric Point: 4.3574

>NTDB_id=1054574 BCU99_RS14145 WP_010435279.1 3125847..3127538(-) (pilQ) [Vibrio cyclitrophicus strain 10N.286.52.F6]
MNRGLQGIVLSVVLFFTVMSHAETSNKLENIDFRVNKDKEAVIIIELATTTAVVDVQRVQEGLSIELINTQVDDDKLYLL
DVKDFATLVEGIEVFRELPSTRLLANISNDYQYEYNLKGRFIEVVVSQPKIDEAAIEKSILEKEGKLISINFQDIPVRNV
LQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEKSRLEEELGE
LKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSMLIRELEENIAVIRGIIESLDIPVKQVQIEARIVTVT
EGNLDELGVRWGVSSNNGSFTVGGSIEGNHPSQITPYDDNGGNSAIDDYLNVNLGATSPNASSIAFQVAKLGSDTLLDLE
LSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPG
QVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSELLIFVTPKV
VIQ

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=1054574 BCU99_RS14145 WP_010435279.1 3125847..3127538(-) (pilQ) [Vibrio cyclitrophicus strain 10N.286.52.F6]
ATGAACAGAGGGCTGCAAGGCATTGTGTTGTCGGTGGTGTTGTTTTTTACGGTGATGAGTCATGCAGAGACCTCCAACAA
GCTGGAGAATATTGATTTCAGGGTCAACAAGGACAAAGAAGCGGTCATCATTATTGAACTGGCAACGACTACTGCGGTTG
TCGATGTTCAGCGAGTGCAAGAAGGGTTGAGTATTGAACTGATCAATACTCAGGTTGACGACGATAAACTGTACCTACTG
GACGTAAAGGACTTTGCGACTTTAGTTGAGGGGATTGAAGTCTTTAGGGAGTTGCCGAGCACTCGGCTGTTGGCAAACAT
CTCCAATGACTACCAATATGAGTACAACCTCAAAGGACGTTTTATTGAGGTTGTTGTGAGTCAGCCTAAGATCGATGAAG
CTGCGATTGAGAAAAGTATCTTAGAAAAAGAGGGCAAACTGATATCGATTAACTTCCAAGATATCCCGGTACGAAATGTC
TTGCAGCTGATTGCCGATTACAATGATTTCAATCTAGTCGTATCTGATTCGGTGGCAGGTAACCTAACTTTACGTTTAGA
TGGCGTACCTTGGCAGCAAGTTCTCGATATTATCTTGCAAGTTAAAGGCTTAGATAAACGTGTTGATGGCAACGTTATCT
TGGTCGCACCGAAAGCGGAACTCGATCTTCGAGAGCAGCAAGCGCTCGAGAAATCGCGTTTAGAAGAGGAGTTGGGTGAA
CTCAAATCTGAAATCATCAAAATCAACTTTGCTAAAGCGACCGACATTGCCGACATGATTGGCGGTGAAGGTGCGGTGAG
TATGTTGTCTGATCGAGGTTCAATTACCATTGATGAACGAACCAACTCAATGCTCATTCGTGAGTTGGAAGAAAACATTG
CGGTGATCCGAGGCATCATTGAATCGCTAGATATTCCGGTGAAGCAAGTTCAGATAGAGGCGCGCATCGTAACTGTCACC
GAGGGTAACCTGGATGAGCTTGGTGTGCGCTGGGGCGTTTCCTCAAACAACGGAAGCTTCACCGTTGGCGGTTCAATTGA
AGGCAACCACCCATCGCAAATTACGCCGTATGACGATAATGGTGGGAATAGTGCGATTGATGATTATCTCAACGTCAATT
TAGGGGCAACATCGCCGAACGCATCGAGTATTGCGTTTCAGGTTGCTAAGTTGGGTTCGGATACGCTGCTCGATCTTGAA
TTATCAGCGCTACAACAAGAATCTAAAGCCGAAATAATTTCGAGCCCACGCTTAATCACCACCAATAAAAAACCGGCTTA
TATTGAGCAAGGTACGGAAATTCCTTATTTGGAGTCCTCTTCAAGTGGTGCAACGTCGGTCGCATTTAAGAAAGCGGTAT
TAAGTTTGAAGGTGACACCGCAGATCACGCCAGATAACCGCTTAGTGTTGGACTTGAGTGTGACTCAAGATAGACCTGGG
CAGGTAGTGAAAACAGGAACGGGCGAAGCAGTGGCCATTGATACTCAACGAATAGGAACGCAAGTGCTTGTTAATAATGG
TGAAACAGTCGTACTTGGCGGGATTTTTCAACATAGTGTCAGCAGTACTGTCGACAAAGTTCCGCTGTTGGGCGACCTGC
CAGTTTTAGGCGCATTGTTCCGTCGCAGCTATGAAAATGTAGGCAAAAGTGAACTGCTTATTTTTGTTACACCTAAAGTT
GTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(244-309)

Bacterial type II and III secretion system protein (Secretin)

(403-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

81.101

96.803

0.785
  pilQ Vibrio cholerae strain A1552

79.963

96.625

0.773
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

79.963

96.625

0.773
  pilQ Legionella pneumophila strain ERS1305867

42.857

96.98

0.416
  pilQ Legionella pneumophila strain Lp02

42.857

96.98

0.416
  pilQ Pseudomonas aeruginosa PAK

40.426

100

0.405
  pilQ Acinetobacter baumannii strain A118

37.167

100

0.396
  pilQ Acinetobacter baumannii D1279779

37.167

100

0.396
  comQ Acinetobacter baylyi ADP1

39.046

100

0.393

Multiple sequence alignment