Detailed information NTG1054578

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BCU99_RS14165
• Genome accession: NZ_CP170015
• Coordinates: 3129323..3130348 (-)
• Length: 341 a.a.
• NCBI ID: WP_016787692.1
• Uniprot ID: A0AAN0LM26
• Organism: Vibrio cyclitrophicus strain 10N.286.52.F6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3124323..3135348

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BCU99_RS14140 (BCU99_14140)	aroK	3125152..3125670 (-)	519	WP_009848907.1	shikimate kinase AroK	-
BCU99_RS14145 (BCU99_14145)	pilQ	3125847..3127538 (-)	1692	WP_010435279.1	type IV pilus secretin PilQ	Machinery gene
BCU99_RS14150 (BCU99_14150)	pilP	3127598..3128116 (-)	519	WP_010435282.1	pilus assembly protein PilP	Machinery gene
BCU99_RS14155 (BCU99_14155)	pilO	3128103..3128732 (-)	630	WP_010435284.1	type 4a pilus biogenesis protein PilO	Machinery gene
BCU99_RS14160 (BCU99_14160)	pilN	3128725..3129339 (-)	615	WP_016790176.1	PilN domain-containing protein	Machinery gene
BCU99_RS14165 (BCU99_14165)	pilM	3129323..3130348 (-)	1026	WP_016787692.1	type IV pilus assembly protein PilM	Machinery gene
BCU99_RS14170 (BCU99_14170)	-	3130500..3133058 (+)	2559	WP_010435294.1	penicillin-binding protein 1A	-
BCU99_RS14175 (BCU99_14175)	oxyR	3133154..3134047 (-)	894	WP_010435297.1	DNA-binding transcriptional regulator OxyR	-
BCU99_RS14180 (BCU99_14180)	-	3134269..3134997 (+)	729	WP_010435300.1	glutathione peroxidase	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38128.77 Da
• Isoelectric Point: 6.8348

>NTDB_id=1054578 BCU99_RS14165 WP_016787692.1 3129323..3130348(-) (pilM) [Vibrio cyclitrophicus strain 10N.286.52.F6]
MGSSLITGIDINHHSIKAVVLKPVGESYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGSRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
IVVDTQAHGLLNIWQLASHLYPEKKNWLLVDVGVDQTSLGIIPQGSAPFYKDIAFGIQDLCSTGNSMDMEGGFSNVEEKH
KFIVNLIEKLKRQLQLYSSVNALQPISGIWLMGEGASMPMVVEELERHFQLSCESLNPLSLFENKVAKKRRLPMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=1054578 BCU99_RS14165 WP_016787692.1 3129323..3130348(-) (pilM) [Vibrio cyclitrophicus strain 10N.286.52.F6]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCACCACAGCATCAAAGCCGTGGTACTAAAACCCGTTGGGGAGTC
GTATGCCCTGGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTCAAAGAACTTAGGAAAGGGCTGCCTTTTGGAAGCCGCAACGTCGCTATTTCGGTACCGGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGCGAATTAGAAGATAGAGAAAAGGAGTTCTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGATCAACAA
GCAGCTATCAAGTGTATGCGACTCGTAAAGAGGTGGTTGATAGCCGAGCTGACGCGCTAACGAAAGCAGGGTTTAAGCCC
ATCGTTGTCGACACTCAGGCCCATGGTTTACTCAACATTTGGCAATTGGCTTCGCATTTGTATCCAGAAAAAAAGAATTG
GCTGTTGGTGGATGTTGGCGTTGATCAAACTTCATTAGGCATTATTCCCCAAGGTTCAGCGCCATTTTATAAAGATATTG
CCTTTGGCATTCAAGATCTTTGCAGTACGGGTAACTCGATGGATATGGAGGGTGGGTTTAGTAACGTTGAGGAGAAGCAT
AAGTTTATTGTCAATTTGATTGAAAAACTCAAGCGTCAGTTACAGCTCTATTCATCGGTCAATGCGCTTCAACCTATCTC
AGGAATTTGGCTAATGGGCGAAGGAGCAAGTATGCCGATGGTCGTTGAAGAGTTAGAGCGACATTTTCAGTTAAGTTGTG
AGTCTTTGAACCCCCTATCTTTGTTTGAGAATAAGGTCGCGAAAAAACGTCGATTACCCATGGATTGGCAACATTTTGGT
ATTGCAGCAGGCATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-220)

• Type IV pilus assembly protein PilM; (PilM_2) (242-328)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	53.392	99.413	0.531
pilM	Vibrio campbellii strain DS40M4	47.661	100	0.478