Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   BCU99_RS14160 Genome accession   NZ_CP170015
Coordinates   3128725..3129339 (-) Length   204 a.a.
NCBI ID   WP_016790176.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.52.F6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3123725..3134339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU99_RS14135 (BCU99_14135) aroB 3124039..3125127 (-) 1089 WP_010435273.1 3-dehydroquinate synthase -
  BCU99_RS14140 (BCU99_14140) aroK 3125152..3125670 (-) 519 WP_009848907.1 shikimate kinase AroK -
  BCU99_RS14145 (BCU99_14145) pilQ 3125847..3127538 (-) 1692 WP_010435279.1 type IV pilus secretin PilQ Machinery gene
  BCU99_RS14150 (BCU99_14150) pilP 3127598..3128116 (-) 519 WP_010435282.1 pilus assembly protein PilP Machinery gene
  BCU99_RS14155 (BCU99_14155) pilO 3128103..3128732 (-) 630 WP_010435284.1 type 4a pilus biogenesis protein PilO Machinery gene
  BCU99_RS14160 (BCU99_14160) pilN 3128725..3129339 (-) 615 WP_016790176.1 PilN domain-containing protein Machinery gene
  BCU99_RS14165 (BCU99_14165) pilM 3129323..3130348 (-) 1026 WP_016787692.1 type IV pilus assembly protein PilM Machinery gene
  BCU99_RS14170 (BCU99_14170) - 3130500..3133058 (+) 2559 WP_010435294.1 penicillin-binding protein 1A -
  BCU99_RS14175 (BCU99_14175) oxyR 3133154..3134047 (-) 894 WP_010435297.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 23373.88 Da        Isoelectric Point: 9.3072

>NTDB_id=1054577 BCU99_RS14160 WP_016790176.1 3128725..3129339(-) (pilN) [Vibrio cyclitrophicus strain 10N.286.52.F6]
MLHQINLLSWRDEIRAQHKKRFIHLVILGVIIALAGQWVVGHYFHDQQAKQQARLSYLDKYIAVLDRQIQSLKVAEQEHK
AILTRLAVVESLQFGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKEVEMHSIV
HNRKRFNKEFQSFKVSFVFSPVSLENTTAEGTTANIKEKGANHG

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1054577 BCU99_RS14160 WP_016790176.1 3128725..3129339(-) (pilN) [Vibrio cyclitrophicus strain 10N.286.52.F6]
ATGTTGCATCAAATTAACCTGTTGTCGTGGCGTGATGAGATTCGCGCTCAGCATAAAAAGCGTTTCATTCATCTGGTCAT
TCTTGGCGTCATCATTGCGCTTGCCGGGCAATGGGTGGTGGGACACTATTTTCATGACCAACAAGCCAAGCAGCAAGCGC
GCCTCAGTTATCTCGATAAGTACATCGCAGTGCTCGACCGACAAATCCAGTCATTAAAAGTCGCAGAGCAAGAGCACAAA
GCCATCCTTACACGACTTGCTGTCGTGGAGTCTTTACAGTTCGGGCGCAATAAAACCACCGACTTTATGAACCTGATGCC
GGAGCTGATTCCTGAGGGCGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATCAGCGACA
GCACCGCTCGTCTAGCCACCATGTTGGACAACCTAGAACGTTCTGACTCTCTTAAAGAAGTTGAGATGCACTCTATTGTT
CATAACCGTAAGCGCTTCAATAAAGAGTTCCAGAGCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTTTCTTTAGAAAACAC
AACCGCAGAGGGCACAACTGCGAACATAAAAGAGAAGGGGGCGAATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

63.212

94.608

0.598
  pilN Vibrio campbellii strain DS40M4

61.78

93.627

0.578

Multiple sequence alignment