Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEOAC_RS23645 Genome accession   NZ_CP168558
Coordinates   5190357..5191493 (+) Length   378 a.a.
NCBI ID   WP_413628611.1    Uniprot ID   -
Organism   Herbaspirillum frisingense strain CT11-119     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5185357..5196493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEOAC_RS23625 (ACEOAC_23625) - 5185621..5186706 (-) 1086 WP_006713198.1 ABC transporter ATP-binding protein -
  ACEOAC_RS23630 (ACEOAC_23630) - 5186975..5187832 (+) 858 WP_241812499.1 IclR family transcriptional regulator -
  ACEOAC_RS23635 (ACEOAC_23635) - 5187829..5188785 (+) 957 WP_413628610.1 2-hydroxyacid dehydrogenase -
  ACEOAC_RS23640 (ACEOAC_23640) - 5188943..5190235 (+) 1293 WP_077235793.1 HlyC/CorC family transporter -
  ACEOAC_RS23645 (ACEOAC_23645) pilF 5190357..5191493 (+) 1137 WP_413628611.1 GspE/PulE family protein Machinery gene
  ACEOAC_RS23650 (ACEOAC_23650) - 5191480..5192721 (+) 1242 WP_413628612.1 type II secretion system F family protein -
  ACEOAC_RS23655 (ACEOAC_23655) pilD 5192802..5193665 (+) 864 WP_413628613.1 prepilin peptidase Machinery gene
  ACEOAC_RS23660 (ACEOAC_23660) coaE 5193675..5194295 (+) 621 WP_413445379.1 dephospho-CoA kinase -
  ACEOAC_RS23665 (ACEOAC_23665) zapD 5194487..5195242 (+) 756 WP_006713184.1 cell division protein ZapD -
  ACEOAC_RS23670 (ACEOAC_23670) yacG 5195272..5195457 (+) 186 WP_413445378.1 DNA gyrase inhibitor YacG -
  ACEOAC_RS23675 (ACEOAC_23675) mutT 5195545..5195958 (-) 414 WP_241812504.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 40937.62 Da        Isoelectric Point: 9.4327

>NTDB_id=1044547 ACEOAC_RS23645 WP_413628611.1 5190357..5191493(+) (pilF) [Herbaspirillum frisingense strain CT11-119]
MQAQTPAVADDDVPVVRLLHKLLADAVGMGASDLHFEPFEHFFRVRLRVDGVLHELAQPPLTLRDKLATRLKILARLDIA
EKRLPQDGKMRLALAQRSIDLRVSTLPTQFGEKIVLRILDTAQASFSIAELGYEPAQQEALLQAIARPHGLVLMTGPTGS
GKTVSLYACLQRLNRPGVNIATAEDPVEINLPGINQVGINERAGLDFATALRAFLRQDPDVLMVGEIRDLETADIAVKAS
QTGHLVLSTLHTNDAPSTLTRLLNMGVPAYNIAASVSLIVAQRLVRKLCRCRRPAPGKPSTYVPQGCPACNQSGYRGRTG
IYQVMPVSAAMQALILAQAGTLELARQAQAEGIIDLRRAGLLKVLRGETRMGEILAGT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1044547 ACEOAC_RS23645 WP_413628611.1 5190357..5191493(+) (pilF) [Herbaspirillum frisingense strain CT11-119]
ATGCAAGCTCAAACCCCGGCGGTGGCCGATGACGATGTCCCGGTAGTACGCCTGCTGCACAAGCTGCTGGCCGACGCCGT
CGGCATGGGTGCCTCCGACCTGCATTTCGAACCCTTCGAACATTTCTTCCGCGTACGCCTGCGCGTCGATGGCGTGCTGC
ACGAACTGGCGCAGCCACCGCTGACACTGCGCGATAAGCTGGCTACCCGCCTGAAGATCCTGGCGCGTCTGGACATCGCC
GAAAAGCGCCTGCCCCAGGACGGCAAGATGCGCCTGGCACTGGCCCAGCGCAGCATCGACCTGCGCGTCTCCACCCTGCC
TACGCAATTCGGCGAAAAGATCGTCCTGCGCATTCTCGATACCGCCCAGGCCAGCTTCTCCATCGCCGAGCTGGGCTATG
AACCGGCCCAGCAGGAAGCGCTGCTGCAAGCCATCGCCCGACCGCATGGGCTGGTGCTGATGACCGGCCCCACCGGCTCG
GGCAAGACGGTCTCACTGTACGCCTGCCTGCAGCGGCTCAACCGCCCCGGGGTCAACATCGCCACTGCCGAGGACCCGGT
GGAAATCAACCTGCCCGGCATCAACCAGGTCGGCATCAACGAACGCGCCGGGCTGGACTTCGCCACGGCGCTGCGCGCCT
TCCTGCGCCAGGACCCGGACGTGCTGATGGTGGGCGAAATCCGCGACCTGGAAACCGCCGACATCGCGGTCAAGGCGTCC
CAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACGACGCCCCCTCGACCCTCACGCGCCTGCTCAACATGGGGGT
GCCGGCCTACAACATCGCTGCGTCGGTGAGCCTGATCGTGGCACAGCGGCTGGTACGCAAGCTGTGCCGCTGCCGGCGTC
CGGCGCCGGGCAAGCCATCCACCTATGTCCCGCAAGGCTGCCCGGCCTGCAACCAGAGCGGCTATCGCGGCCGCACCGGA
ATCTACCAGGTGATGCCGGTCAGCGCCGCCATGCAGGCGCTGATCCTGGCCCAGGCCGGCACGCTGGAGCTGGCACGCCA
GGCGCAGGCCGAGGGCATCATCGACCTGCGCCGCGCCGGGCTGCTGAAGGTCCTGCGCGGGGAAACCCGCATGGGAGAGA
TACTTGCCGGCACCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(22-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

55.784

100

0.574
  pilB Vibrio parahaemolyticus RIMD 2210633

54.974

100

0.556
  pilB Vibrio campbellii strain DS40M4

54.712

100

0.553
  pilB Acinetobacter baumannii D1279779

54.167

100

0.55
  pilB Acinetobacter baylyi ADP1

54.593

100

0.55
  pilB Vibrio cholerae strain A1552

54.047

100

0.548
  pilB Legionella pneumophila strain ERS1305867

50.643

100

0.521
  pilB Haemophilus influenzae 86-028NP

46.791

98.942

0.463
  pilB Haemophilus influenzae Rd KW20

46.524

98.942

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

42.893

100

0.447
  pilF Thermus thermophilus HB27

42.597

100

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.228

100

0.431
  pilB Glaesserella parasuis strain SC1401

49.371

84.127

0.415
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.37

100

0.397

Multiple sequence alignment