Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACEN8Q_RS27420 Genome accession   NZ_CP168522
Coordinates   6026345..6027562 (-) Length   405 a.a.
NCBI ID   WP_413426323.1    Uniprot ID   -
Organism   Pseudomonas sp. Pseusp16 strain CT11-16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6021345..6032562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN8Q_RS27390 (ACEN8Q_27385) - 6022504..6022746 (-) 243 WP_413426335.1 DUF2790 domain-containing protein -
  ACEN8Q_RS27395 (ACEN8Q_27390) - 6022868..6023800 (+) 933 WP_413703999.1 LysR family transcriptional regulator -
  ACEN8Q_RS27400 (ACEN8Q_27395) - 6023929..6024618 (+) 690 WP_413704000.1 energy-coupling factor ABC transporter permease -
  ACEN8Q_RS27405 (ACEN8Q_27400) yacG 6024654..6024854 (-) 201 WP_413426329.1 DNA gyrase inhibitor YacG -
  ACEN8Q_RS27410 (ACEN8Q_27405) coaE 6024851..6025474 (-) 624 WP_413426327.1 dephospho-CoA kinase -
  ACEN8Q_RS27415 (ACEN8Q_27410) pilD 6025471..6026343 (-) 873 WP_413426325.1 prepilin peptidase Machinery gene
  ACEN8Q_RS27420 (ACEN8Q_27415) pilC 6026345..6027562 (-) 1218 WP_413426323.1 type II secretion system F family protein Machinery gene
  ACEN8Q_RS27425 (ACEN8Q_27420) - 6027790..6028203 (+) 414 WP_413704001.1 pilin -
  ACEN8Q_RS27430 (ACEN8Q_27425) - 6028504..6028860 (+) 357 WP_413426319.1 BON domain-containing protein -
  ACEN8Q_RS27435 (ACEN8Q_27430) - 6029040..6029336 (-) 297 WP_413426317.1 DUF2845 domain-containing protein -
  ACEN8Q_RS27440 (ACEN8Q_27435) - 6029336..6032293 (-) 2958 WP_413704002.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44352.15 Da        Isoelectric Point: 10.1093

>NTDB_id=1044471 ACEN8Q_RS27420 WP_413426323.1 6026345..6027562(-) (pilC) [Pseudomonas sp. Pseusp16 strain CT11-16]
MAVKAAKISVYAWEGIDRKGTKVTGELSAQNPALLKAKLRKQGINPGKVRKKNQAVFNMGKRIKAQDIALFTRQMATMMR
AGVPLLQSFDIIGEGFDNPAMRQLVDELKQEVAAGNSFATALRKQPRYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKARIRKAMTYPLVVVFVAAIVTGILLVNVVPQFESVFKSFGAELPAFTVMVIGLSQFMQAWWWTVLGALIAMAFGVR
HALRTSPAFRDRMDTWLLKLPLVGTLTYKSAVARFARTLSTTFAAGVPLLEALESVAGATGNIVFKRAVLRIRQDVSTGM
QLNFAMRTSGVFPNMAIQMTAIGEESGALDGMLEKVADFYENDVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1044471 ACEN8Q_RS27420 WP_413426323.1 6026345..6027562(-) (pilC) [Pseudomonas sp. Pseusp16 strain CT11-16]
ATGGCGGTCAAGGCAGCGAAAATCAGCGTGTACGCATGGGAAGGCATCGACCGCAAAGGCACAAAGGTAACCGGTGAGCT
CAGCGCTCAAAACCCGGCACTGCTCAAGGCGAAACTGCGCAAACAAGGCATCAATCCGGGCAAGGTTCGCAAGAAAAACC
AAGCGGTTTTCAACATGGGCAAGCGCATCAAGGCCCAGGACATTGCCCTGTTCACCCGGCAAATGGCAACGATGATGAGG
GCCGGCGTGCCCTTGTTACAGTCGTTCGACATCATCGGCGAAGGCTTCGACAATCCGGCCATGCGCCAGCTGGTGGACGA
ACTGAAACAGGAAGTCGCGGCCGGCAACAGTTTCGCCACCGCCCTGCGCAAGCAGCCCCGGTATTTCGATGAACTGTATT
GCAACCTGGTGGATGCCGGCGAGCAGGCCGGAGCCCTCGACACCCTTTTGGAGCGGGTCGCGACCTATAAGGAAAAAAGC
GAAAGCCTCAAGGCCAGGATCAGGAAAGCCATGACCTACCCGCTGGTGGTGGTGTTCGTCGCGGCGATTGTCACCGGGAT
TCTGCTGGTCAACGTCGTACCGCAGTTCGAATCGGTGTTCAAAAGCTTTGGCGCCGAACTGCCGGCCTTCACCGTGATGG
TCATTGGCTTGTCGCAATTCATGCAGGCCTGGTGGTGGACGGTCCTTGGCGCGCTGATCGCAATGGCCTTCGGTGTGCGT
CACGCCCTCAGGACATCCCCGGCCTTTCGCGACCGGATGGACACCTGGCTGCTGAAACTGCCGCTGGTGGGCACGTTGAC
TTACAAGTCCGCCGTGGCCCGGTTCGCCCGTACACTGTCGACTACATTCGCTGCCGGCGTGCCACTGCTGGAGGCCCTGG
AATCAGTGGCCGGTGCGACCGGCAATATCGTGTTCAAGCGCGCCGTGTTGCGCATCCGGCAAGACGTTTCAACCGGCATG
CAATTGAATTTCGCCATGCGTACCTCTGGCGTGTTTCCGAACATGGCCATACAGATGACCGCCATCGGCGAAGAGTCCGG
CGCCCTGGACGGTATGCTCGAAAAAGTCGCGGATTTCTACGAAAACGACGTCGATAACATGGTCGATAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGTGTTATCGTCGGTGGACTGGTAGTTGCCATGTACCTGCCCATTTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

70.123

100

0.701
  pilC Acinetobacter baylyi ADP1

58.637

100

0.595
  pilC Acinetobacter baumannii D1279779

57.598

100

0.58
  pilC Legionella pneumophila strain ERS1305867

53.788

97.778

0.526
  pilC Vibrio cholerae strain A1552

42.929

97.778

0.42
  pilG Neisseria meningitidis 44/76-A

41.481

100

0.415
  pilG Neisseria gonorrhoeae MS11

41.542

99.259

0.412
  pilC Vibrio campbellii strain DS40M4

40.394

100

0.405
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment