Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   P6F15_RS01055 Genome accession   NZ_CP168247
Coordinates   201298..202515 (+) Length   405 a.a.
NCBI ID   WP_053105045.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain 177     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 196298..207515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P6F15_RS01030 (P6F15_01030) rplQ 197176..197559 (+) 384 WP_053100999.1 50S ribosomal protein L17 -
  P6F15_RS01035 (P6F15_01035) - 197638..198054 (+) 417 WP_053103338.1 PACE efflux transporter -
  P6F15_RS01040 (P6F15_01040) elbB 198097..198750 (+) 654 WP_373332287.1 isoprenoid biosynthesis glyoxalase ElbB -
  P6F15_RS01045 (P6F15_01045) - 198821..199330 (-) 510 WP_053105043.1 pilin -
  P6F15_RS01050 (P6F15_01050) pilB 199601..201292 (+) 1692 WP_373332288.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P6F15_RS01055 (P6F15_01055) pilC 201298..202515 (+) 1218 WP_053105045.1 type II secretion system F family protein Machinery gene
  P6F15_RS01060 (P6F15_01060) pilD 202519..203418 (+) 900 WP_373332289.1 A24 family peptidase Machinery gene
  P6F15_RS01065 (P6F15_01065) - 203626..203907 (-) 282 WP_053109901.1 GIY-YIG nuclease family protein -
  P6F15_RS01070 (P6F15_01070) - 204172..205161 (-) 990 WP_373332290.1 hypothetical protein -
  P6F15_RS01075 (P6F15_01075) - 205328..206539 (-) 1212 WP_053105049.1 NAD(P)/FAD-dependent oxidoreductase -
  P6F15_RS01080 (P6F15_01080) - 206536..206994 (-) 459 WP_053103346.1 YiiD C-terminal domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44118.95 Da        Isoelectric Point: 9.7844

>NTDB_id=1043451 P6F15_RS01055 WP_053105045.1 201298..202515(+) (pilC) [Thiopseudomonas alkaliphila strain 177]
MEIKKSPKSYMYVWHGKSRQGNKLSGVINSHDINIVKAQLRKQGVLHAKVKKKGITFGRDKKIKSMDITVFTRQMATMMQ
AGVPLLQSFTIIIEGLDNSNMRKLLEAVRNDIAGGSSFAGALRKHSLYFDELYCNLVASGETSGALETLLDRVATYKEKS
EALKSKIKKAMNYPIAVVVVAAVVTSILLLKVVPQFQEIFEGFGAELPVFTQLVIGISELLQEYSLYVLIGLVLLLYAAR
RTLRRSEQLRNRADKILLKMPIIGDILYNSAVARYGRTLATTFSAGVPLVDALDSVSGAIGNVVFKSAVQQVKSDVSTGM
QLHTSMMSSGVFPPLALQLTAIGEESGALDTMLEKVAAYYEAEVDNAVDGLTALLEPIIMVVLGVLIGGLVIAMYLPILQ
MGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1043451 P6F15_RS01055 WP_053105045.1 201298..202515(+) (pilC) [Thiopseudomonas alkaliphila strain 177]
ATGGAAATTAAGAAGTCGCCTAAAAGCTATATGTATGTTTGGCATGGTAAAAGCAGGCAAGGGAATAAGTTAAGTGGTGT
TATTAATTCGCATGATATTAATATTGTAAAGGCTCAGCTAAGAAAGCAGGGAGTTCTGCATGCTAAGGTGAAAAAGAAAG
GTATCACCTTTGGTCGCGATAAAAAAATTAAATCCATGGACATAACCGTCTTTACTCGGCAAATGGCCACCATGATGCAA
GCCGGGGTGCCTTTATTACAGTCGTTTACCATTATTATTGAAGGCTTAGACAATTCCAATATGCGTAAATTACTAGAAGC
TGTGCGTAATGATATTGCTGGGGGTAGCAGTTTTGCAGGAGCTTTACGCAAACACTCACTATATTTTGATGAGTTGTACT
GCAATTTAGTGGCCTCAGGCGAAACGTCGGGAGCTTTGGAAACTTTGCTTGATCGAGTAGCGACCTATAAAGAAAAGTCA
GAGGCGCTTAAATCTAAAATTAAAAAAGCCATGAACTACCCGATTGCTGTAGTGGTGGTGGCAGCTGTTGTGACGAGTAT
TTTGCTATTAAAAGTGGTACCACAGTTCCAGGAGATCTTTGAAGGTTTTGGGGCTGAATTGCCTGTATTTACGCAATTGG
TAATTGGTATTTCAGAGTTGCTGCAAGAGTATAGTTTATATGTGCTGATAGGCTTAGTACTGTTACTTTATGCTGCTCGT
AGGACGTTGCGCCGATCAGAGCAGTTGCGCAATCGTGCGGATAAAATACTGCTAAAAATGCCGATAATAGGTGATATTTT
ATATAACTCTGCTGTGGCTCGTTATGGGCGCACCTTAGCTACAACCTTCTCAGCCGGCGTACCTTTGGTTGATGCGTTAG
ATTCAGTTTCTGGCGCAATCGGCAACGTGGTTTTTAAAAGTGCAGTGCAGCAAGTGAAGTCCGATGTGTCTACTGGTATG
CAGTTGCATACTTCAATGATGTCTAGCGGGGTATTTCCTCCCCTAGCACTACAACTTACTGCTATTGGTGAAGAGTCTGG
TGCGCTAGATACCATGTTAGAAAAAGTTGCAGCGTACTATGAAGCGGAAGTAGATAACGCTGTGGATGGCTTAACTGCGT
TGCTAGAGCCAATTATTATGGTGGTGCTAGGTGTACTAATTGGCGGACTAGTTATTGCCATGTACCTGCCAATCTTACAA
ATGGGTAACGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.261

99.506

0.649
  pilC Acinetobacter baumannii D1279779

54.657

100

0.551
  pilC Acinetobacter baylyi ADP1

53.659

100

0.543
  pilC Legionella pneumophila strain ERS1305867

49.257

99.753

0.491
  pilG Neisseria gonorrhoeae MS11

43.108

98.519

0.425
  pilG Neisseria meningitidis 44/76-A

42.857

98.519

0.422
  pilC Vibrio campbellii strain DS40M4

41.791

99.259

0.415
  pilC Vibrio cholerae strain A1552

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

39.599

98.519

0.39

Multiple sequence alignment