Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P6F15_RS01050 Genome accession   NZ_CP168247
Coordinates   199601..201292 (+) Length   563 a.a.
NCBI ID   WP_373332288.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain 177     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 194601..206292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P6F15_RS01010 (P6F15_01010) rpsM 194693..195049 (+) 357 WP_053100995.1 30S ribosomal protein S13 -
  P6F15_RS01015 (P6F15_01015) rpsK 195080..195469 (+) 390 WP_053100996.1 30S ribosomal protein S11 -
  P6F15_RS01020 (P6F15_01020) rpsD 195488..196108 (+) 621 WP_053100997.1 30S ribosomal protein S4 -
  P6F15_RS01025 (P6F15_01025) rpoA 196131..197132 (+) 1002 WP_053100998.1 DNA-directed RNA polymerase subunit alpha -
  P6F15_RS01030 (P6F15_01030) rplQ 197176..197559 (+) 384 WP_053100999.1 50S ribosomal protein L17 -
  P6F15_RS01035 (P6F15_01035) - 197638..198054 (+) 417 WP_053103338.1 PACE efflux transporter -
  P6F15_RS01040 (P6F15_01040) elbB 198097..198750 (+) 654 WP_373332287.1 isoprenoid biosynthesis glyoxalase ElbB -
  P6F15_RS01045 (P6F15_01045) - 198821..199330 (-) 510 WP_053105043.1 pilin -
  P6F15_RS01050 (P6F15_01050) pilB 199601..201292 (+) 1692 WP_373332288.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P6F15_RS01055 (P6F15_01055) pilC 201298..202515 (+) 1218 WP_053105045.1 type II secretion system F family protein Machinery gene
  P6F15_RS01060 (P6F15_01060) pilD 202519..203418 (+) 900 WP_373332289.1 A24 family peptidase Machinery gene
  P6F15_RS01065 (P6F15_01065) - 203626..203907 (-) 282 WP_053109901.1 GIY-YIG nuclease family protein -
  P6F15_RS01070 (P6F15_01070) - 204172..205161 (-) 990 WP_373332290.1 hypothetical protein -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62422.44 Da        Isoelectric Point: 6.2895

>NTDB_id=1043450 P6F15_RS01050 WP_373332288.1 199601..201292(+) (pilB) [Thiopseudomonas alkaliphila strain 177]
MELSSGLIGLAGQIVKLGLADYKKVLNSVADARLAGQPIISWLVHNKIVTSREVMTLATSQFGVASFDLSCFKIKLLSKD
LLSERIVRKHRVLPLAKRGNLLYVALADPGDQLVLNEIKFTTGCAVELVVVEQDKLDLLIEQLYDSLLESFESLADEVLE
ESEYIEIQNELIIDAIDEAPIVKFVNSMLLRAIKIGASDLHFEPYENSYRVRFRTDGILHEVSKQPVGLRDRISTRLKVM
ASIDISERRKPQDGRIKLKVSSVKSINFRVNTLPTLWGEKIVLRILDSDSTKLGIDSLGYESAQKKLYLEALDLPQGMIL
VTGPTGSGKSVSLYTGINIINTVERNISTAEDPVEIYLEGINQVNVNFKQGLDFAQALRAFLRQDPDVIMIGEIRDLETA
EIAIKAAQTGHMVMSTLHTNSAADTLTRLRNMGVAAFNLAISINLIIAQRLARRLCNICKSVKKIPVDILLQEGFSLEQV
GTFKVYEAVGCDKCNAGYKGRIGIYEVVKITPTLQAIIMEGGNPIEINQQIRKEGFNDLRTSALEKVIQGVVSLEEVNRV
TKD

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=1043450 P6F15_RS01050 WP_373332288.1 199601..201292(+) (pilB) [Thiopseudomonas alkaliphila strain 177]
ATGGAGTTATCAAGCGGCTTAATAGGTCTGGCAGGACAAATTGTCAAATTAGGCTTGGCAGACTATAAAAAGGTTTTAAA
CTCTGTTGCAGATGCTAGGCTGGCGGGCCAGCCAATTATTTCTTGGCTAGTGCATAATAAAATTGTCACTTCTCGAGAAG
TGATGACATTAGCTACAAGCCAATTTGGAGTAGCTAGTTTTGATTTAAGCTGTTTTAAAATAAAATTACTGAGTAAAGAT
TTACTTAGTGAAAGAATTGTTCGTAAACACCGAGTATTACCTTTAGCTAAGCGAGGCAATCTGCTTTATGTTGCCTTGGC
AGACCCTGGAGATCAGCTTGTACTTAATGAAATAAAATTTACGACTGGCTGTGCTGTTGAGCTAGTCGTAGTAGAGCAAG
ATAAATTGGATCTGCTTATTGAGCAACTTTATGACAGCTTGCTTGAGAGTTTTGAAAGTCTGGCTGATGAAGTCTTAGAA
GAGTCTGAGTATATTGAAATACAAAATGAGCTAATAATAGATGCTATAGATGAGGCTCCTATAGTTAAATTTGTAAATAG
TATGTTATTGCGTGCTATTAAAATAGGTGCTTCAGATTTACATTTTGAACCCTATGAAAATAGTTATAGAGTTCGCTTTC
GTACTGATGGGATATTGCATGAAGTAAGCAAGCAGCCTGTAGGTTTACGTGATCGGATCTCTACACGGCTCAAGGTTATG
GCTAGCATTGATATTTCTGAGCGTCGTAAACCACAAGACGGAAGAATTAAACTAAAGGTATCATCGGTAAAGTCTATAAA
TTTTCGAGTTAATACTTTACCTACTTTATGGGGTGAAAAAATTGTGCTAAGGATTTTAGATTCTGATAGTACAAAACTAG
GAATTGACTCGTTAGGCTATGAGTCAGCACAAAAAAAGCTTTATTTAGAAGCTTTAGATTTACCCCAAGGTATGATTTTA
GTGACTGGGCCTACTGGCTCGGGTAAAAGTGTATCTTTATATACAGGAATCAATATTATCAACACTGTAGAACGTAACAT
TTCTACTGCAGAAGATCCTGTGGAAATCTATTTGGAAGGGATTAATCAAGTTAATGTTAACTTCAAGCAAGGACTGGATT
TTGCACAAGCTTTGCGTGCTTTTTTACGACAGGATCCTGATGTAATAATGATTGGAGAAATTCGTGACTTAGAAACTGCA
GAAATTGCAATTAAGGCGGCACAAACTGGACACATGGTAATGTCAACTTTACATACAAATAGTGCTGCAGACACTCTTAC
ACGTCTACGTAATATGGGAGTAGCAGCCTTTAATTTAGCTATTTCGATTAATTTAATCATTGCCCAACGGTTAGCTAGAC
GTTTATGTAATATATGTAAGTCTGTTAAGAAAATACCAGTTGATATTTTATTGCAGGAGGGTTTTAGCTTAGAGCAAGTA
GGCACTTTTAAGGTATATGAGGCTGTTGGCTGTGATAAGTGTAATGCGGGATATAAAGGGCGAATTGGAATTTATGAAGT
GGTGAAAATTACACCTACATTACAAGCTATTATTATGGAAGGGGGGAATCCTATTGAAATTAACCAACAAATACGTAAAG
AAGGTTTTAATGATCTACGCACTTCAGCGTTAGAGAAGGTAATTCAGGGAGTTGTTAGTTTAGAAGAGGTGAATCGAGTA
ACTAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(187-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.204

99.29

0.538
  pilB Acinetobacter baumannii D1279779

55.801

96.448

0.538
  pilB Legionella pneumophila strain ERS1305867

52.72

94.671

0.499
  pilF Neisseria gonorrhoeae MS11

48.972

95.027

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

47.292

98.401

0.465
  pilB Vibrio campbellii strain DS40M4

46.085

99.822

0.46
  pilB Vibrio cholerae strain A1552

47.932

94.494

0.453
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.091

97.691

0.362

Multiple sequence alignment