Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACCQ12_RS14975 Genome accession   NZ_CP167863
Coordinates   3553544..3553954 (-) Length   136 a.a.
NCBI ID   WP_249048336.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 1068     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3548544..3558954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ12_RS14955 (ACCQ12_14955) - 3549037..3549414 (-) 378 WP_372382094.1 EamA family transporter -
  ACCQ12_RS14960 (ACCQ12_14960) - 3549411..3550202 (-) 792 WP_372382095.1 glycosyltransferase family 2 protein -
  ACCQ12_RS14965 (ACCQ12_14965) - 3550204..3551394 (-) 1191 WP_372382096.1 glycosyltransferase family 2 protein -
  ACCQ12_RS14970 (ACCQ12_14970) - 3551387..3553471 (-) 2085 WP_372382097.1 hypothetical protein -
  ACCQ12_RS14975 (ACCQ12_14975) pilE 3553544..3553954 (-) 411 WP_249048336.1 pilin Machinery gene
  ACCQ12_RS14980 (ACCQ12_14980) pilC 3554360..3555619 (+) 1260 WP_372383630.1 type II secretion system F family protein Machinery gene
  ACCQ12_RS14985 (ACCQ12_14985) - 3555626..3556489 (+) 864 WP_046343941.1 A24 family peptidase -
  ACCQ12_RS14990 (ACCQ12_14990) coaE 3556503..3557126 (+) 624 WP_372382098.1 dephospho-CoA kinase -
  ACCQ12_RS14995 (ACCQ12_14995) - 3557174..3557382 (+) 209 Protein_2929 SymE family type I addiction module toxin -
  ACCQ12_RS15000 (ACCQ12_15000) - 3557484..3558815 (-) 1332 WP_166744818.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14106.17 Da        Isoelectric Point: 9.0496

>NTDB_id=1039974 ACCQ12_RS14975 WP_249048336.1 3553544..3553954(-) (pilE) [Xanthomonas sp. NCPPB 1068]
MKKNQQGFTLIELMIVVAIIAILAAIALPAYSDYTKKAKVSEVILAASSARTAVAEFAAGTGALPNASWTPETQSSKYVN
GIAWDGSKITATAQGIPSVSGNITLTANMNATTQQIDWTCGGSIPAKYRPGTCQGP

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1039974 ACCQ12_RS14975 WP_249048336.1 3553544..3553954(-) (pilE) [Xanthomonas sp. NCPPB 1068]
ATGAAGAAGAATCAGCAGGGCTTTACCCTGATCGAATTGATGATCGTTGTTGCGATCATCGCCATCCTGGCTGCTATCGC
GCTGCCGGCCTATTCCGACTACACGAAGAAGGCGAAGGTGTCCGAAGTGATCTTGGCTGCTTCCTCGGCTCGTACCGCTG
TCGCCGAGTTTGCTGCCGGTACCGGCGCGCTGCCGAATGCCAGCTGGACACCTGAAACGCAGTCCTCAAAGTATGTCAAT
GGCATCGCCTGGGATGGCTCTAAGATCACTGCCACAGCACAGGGCATCCCGAGTGTGTCAGGTAATATCACTCTCACTGC
CAACATGAACGCTACCACCCAGCAGATCGATTGGACTTGTGGTGGCTCGATTCCCGCTAAGTATCGTCCGGGTACCTGCC
AGGGTCCGTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.362

100

0.544
  pilA2 Legionella pneumophila strain ERS1305867

45.775

100

0.478
  comP Acinetobacter baylyi ADP1

43.537

100

0.471
  pilA2 Legionella pneumophila str. Paris

44.681

100

0.463
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.559

100

0.426
  pilE Neisseria gonorrhoeae strain FA1090

35

100

0.412
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.007

100

0.404
  pilE Neisseria gonorrhoeae MS11

34.591

100

0.404
  pilA Acinetobacter baumannii strain A118

39.568

100

0.404

Multiple sequence alignment