Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AB9V60_RS03615 Genome accession   NZ_CP166624
Coordinates   836773..837990 (-) Length   405 a.a.
NCBI ID   WP_370683305.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. atrofaciens strain GM 2231     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 831773..842990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9V60_RS03585 (AB9V60_03585) - 833199..833732 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  AB9V60_RS03590 (AB9V60_03590) - 833729..834358 (-) 630 WP_003392710.1 DUF1780 domain-containing protein -
  AB9V60_RS03595 (AB9V60_03595) - 834485..834946 (+) 462 WP_029573726.1 hypothetical protein -
  AB9V60_RS03600 (AB9V60_03600) yacG 835073..835282 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  AB9V60_RS03605 (AB9V60_03605) coaE 835279..835902 (-) 624 WP_029572866.1 dephospho-CoA kinase -
  AB9V60_RS03610 (AB9V60_03610) pilD 835899..836771 (-) 873 WP_129430362.1 A24 family peptidase Machinery gene
  AB9V60_RS03615 (AB9V60_03615) pilC 836773..837990 (-) 1218 WP_370683305.1 type II secretion system F family protein Machinery gene
  AB9V60_RS03620 (AB9V60_03620) pilB 837993..839687 (-) 1695 WP_003414827.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB9V60_RS03625 (AB9V60_03625) - 839912..840328 (+) 417 WP_032697899.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AB9V60_RS03630 (AB9V60_03630) - 840681..841919 (+) 1239 WP_370683306.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44024.75 Da        Isoelectric Point: 10.0245

>NTDB_id=1034843 AB9V60_RS03615 WP_370683305.1 836773..837990(-) (pilC) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
MASKSVKVSVYTWEGVDKKGGKLSGEVNGHNLALVKAQLRKQGINVTKVRKKPVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIIAEGAENPNMRALVDSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAVIVSGILLIKVVPQFQSVFTGFGAELPAFTLMVIGLSNIVQEWWLGIVGLFFFGFFVSK
KSYKQSQKFRDSIDRFLLKVPVIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDNMLDKVATYYEEEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1034843 AB9V60_RS03615 WP_370683305.1 836773..837990(-) (pilC) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
ATGGCCAGCAAGTCAGTTAAAGTCAGTGTTTACACATGGGAAGGTGTGGACAAGAAAGGCGGCAAACTCAGTGGAGAAGT
GAACGGGCACAATCTTGCGCTTGTGAAGGCGCAGTTGCGCAAACAAGGTATCAACGTCACCAAGGTACGCAAAAAGCCCG
TATCAATCTTTGGCAAAGGCAAGAAGATCAAGCCGCTGGACATCGCATTCTTTTCTCGGCAGATGGCAACCATGATGAAG
GCAGGTGTACCGCTTCTGCAGTCATTTGACATTATTGCCGAGGGCGCAGAAAACCCGAACATGCGTGCGCTAGTCGATTC
ACTAAAGCAGGAGGTTTCAGCAGGGAACAGCTTTGCGACTGCTCTGCGACAGAAACCGGAATACTTCGATGACCTGTTTT
GCAATTTGGTAGATGCTGGGGAGCAGGCTGGAGCGCTAGAGAGCCTGCTGGACCGAGTGGCTAGCTATAAGGAAAAGACA
GAAAAGCTGAAGGCAAAAATTAAAAAAGCCATGACTTATCCTGCCGCTGTTTTGATAGTGGCCGTTATTGTTTCAGGCAT
CTTATTAATTAAAGTAGTACCACAATTTCAATCTGTATTTACCGGTTTTGGTGCAGAGCTACCGGCGTTCACACTGATGG
TCATCGGACTGTCTAACATCGTACAGGAGTGGTGGTTAGGCATAGTTGGATTATTTTTTTTCGGTTTCTTCGTATCCAAA
AAATCTTATAAGCAGTCCCAAAAATTTCGAGACAGCATTGATCGATTTTTGTTGAAAGTGCCTGTCATAGGACCACTTAT
TTTCAAGTCTTCAGTAGCTCGCTACGCCCGAACGCTTGCAACGACATTTGCAGCAGGCGTCCCGTTGGTAGAAGCACTTG
ATTCAGTTGCAGGTGCTACTGGTAACGTGGTTTTTAGAAACGCGGTCAATCAGGTAAAACAGGATGTCTCCACGGGCATG
CAACTGAATTTCTCAATGCGCTCGACCGGTGTTTTCCCGAGTCTGGCGATTCAGATGACCGCTATTGGCGAGGAATCAGG
CGCACTGGACAATATGCTCGATAAAGTCGCGACCTATTACGAAGAAGAAGTCGACAACATGGTCGATAGTCTAACCAGCT
TGATGGAACCCATGATCATGGCTGTGCTGGGTGTCATCGTCGGCGGCCTTGTCATTGCGATGTACCTCCCCATTTTCAAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.086

100

0.731
  pilC Acinetobacter baylyi ADP1

59.367

100

0.602
  pilC Acinetobacter baumannii D1279779

58.168

99.753

0.58
  pilC Legionella pneumophila strain ERS1305867

52.078

100

0.526
  pilG Neisseria gonorrhoeae MS11

42.607

98.519

0.42
  pilC Vibrio campbellii strain DS40M4

42.821

98.025

0.42
  pilG Neisseria meningitidis 44/76-A

42.356

98.519

0.417
  pilC Vibrio cholerae strain A1552

41.235

100

0.412
  pilC Thermus thermophilus HB27

39.801

99.259

0.395

Multiple sequence alignment