Detailed information NTG1034842

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AB9V60_RS03610
• Genome accession: NZ_CP166624
• Coordinates: 835899..836771 (-)
• Length: 290 a.a.
• NCBI ID: WP_129430362.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. atrofaciens strain GM 2231
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 830899..841771

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB9V60_RS03580 (AB9V60_03580)	-	831684..832982 (-)	1299	WP_003392712.1	NAD(P)/FAD-dependent oxidoreductase	-
AB9V60_RS03585 (AB9V60_03585)	-	833199..833732 (-)	534	WP_003317783.1	MOSC domain-containing protein	-
AB9V60_RS03590 (AB9V60_03590)	-	833729..834358 (-)	630	WP_003392710.1	DUF1780 domain-containing protein	-
AB9V60_RS03595 (AB9V60_03595)	-	834485..834946 (+)	462	WP_029573726.1	hypothetical protein	-
AB9V60_RS03600 (AB9V60_03600)	yacG	835073..835282 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
AB9V60_RS03605 (AB9V60_03605)	coaE	835279..835902 (-)	624	WP_029572866.1	dephospho-CoA kinase	-
AB9V60_RS03610 (AB9V60_03610)	pilD	835899..836771 (-)	873	WP_129430362.1	A24 family peptidase	Machinery gene
AB9V60_RS03615 (AB9V60_03615)	pilC	836773..837990 (-)	1218	WP_370683305.1	type II secretion system F family protein	Machinery gene
AB9V60_RS03620 (AB9V60_03620)	pilB	837993..839687 (-)	1695	WP_003414827.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AB9V60_RS03625 (AB9V60_03625)	-	839912..840328 (+)	417	WP_032697899.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31979.16 Da
• Isoelectric Point: 8.3734

>NTDB_id=1034842 AB9V60_RS03610 WP_129430362.1 835899..836771(-) (pilD) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRIPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLGWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=1034842 AB9V60_RS03610 WP_129430362.1 835899..836771(-) (pilD) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
ATGCCCCTCCTCGACCTACTGGCCAGCTCCCCCCTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCATCCCGATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGCGAACTGCTCGGCC
TGCCCGCCGAACCCGATCAGCCCGTCTTCAACCTGAACCGCCCCCGTTCCAGCTGCCCGCATTGCGCACACAAAATCCGC
CCATGGGAAAACCTGCCGGTCATCAGCTATCTGCTGCTGCGCGGCAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCGGTGCTATCAGCCTACGTGGCATGGCATTTCGGCTTTGGCTGGCAGGCGGCGG
CGATGCTGGTACTGGGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGTTGTTACCTGATTCTCTGGTG
CTGCCACTGCTGTGGCTAGGCCTCATAGTCAACGCTTTCGGGCTGTTCACATCGCTGAACGATGCGCTTTGGGGCGCGGT
GGCCGGTTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTTACAGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCGCTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTTGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.65	97.586	0.514
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	51.825	94.483	0.49
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476