Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB9V60_RS03620 Genome accession   NZ_CP166624
Coordinates   837993..839687 (-) Length   564 a.a.
NCBI ID   WP_003414827.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. atrofaciens strain GM 2231     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 832993..844687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9V60_RS03585 (AB9V60_03585) - 833199..833732 (-) 534 WP_003317783.1 MOSC domain-containing protein -
  AB9V60_RS03590 (AB9V60_03590) - 833729..834358 (-) 630 WP_003392710.1 DUF1780 domain-containing protein -
  AB9V60_RS03595 (AB9V60_03595) - 834485..834946 (+) 462 WP_029573726.1 hypothetical protein -
  AB9V60_RS03600 (AB9V60_03600) yacG 835073..835282 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  AB9V60_RS03605 (AB9V60_03605) coaE 835279..835902 (-) 624 WP_029572866.1 dephospho-CoA kinase -
  AB9V60_RS03610 (AB9V60_03610) pilD 835899..836771 (-) 873 WP_129430362.1 A24 family peptidase Machinery gene
  AB9V60_RS03615 (AB9V60_03615) pilC 836773..837990 (-) 1218 WP_370683305.1 type II secretion system F family protein Machinery gene
  AB9V60_RS03620 (AB9V60_03620) pilB 837993..839687 (-) 1695 WP_003414827.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB9V60_RS03625 (AB9V60_03625) - 839912..840328 (+) 417 WP_032697899.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AB9V60_RS03630 (AB9V60_03630) - 840681..841919 (+) 1239 WP_370683306.1 O-antigen ligase family protein -
  AB9V60_RS03635 (AB9V60_03635) - 842031..843317 (+) 1287 WP_370683307.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  AB9V60_RS03640 (AB9V60_03640) - 843367..843912 (-) 546 WP_010415546.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62513.75 Da        Isoelectric Point: 6.0156

>NTDB_id=1034844 AB9V60_RS03620 WP_003414827.1 837993..839687(-) (pilB) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
MTDVVLTGLAKQLVLAELLTETAAQQAYQQARREKVSLVSYLVQNKLVRSLTLAEIASDQFGVPFVDLSSLDKESQPKGL
VSEKLVRQHNALPLWRRGNKLFIGISDPTNHQAITDIQFSTGLNTEAILVEDDKLTVAIDRFFDTESGLGELADIDLGLE
IEPASDKETSLATQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVAKPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKNKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEEK
VGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1034844 AB9V60_RS03620 WP_003414827.1 837993..839687(-) (pilB) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
ATGACTGATGTTGTTCTCACTGGTTTGGCCAAACAACTGGTCCTGGCCGAGCTCCTTACAGAAACTGCCGCGCAGCAGGC
CTATCAGCAGGCTCGCCGCGAAAAAGTCTCGCTGGTTAGCTACCTCGTACAAAACAAATTGGTCAGAAGCCTGACGCTGG
CGGAAATAGCATCCGATCAGTTCGGCGTTCCATTCGTAGATCTTTCAAGCCTGGACAAGGAAAGCCAGCCTAAAGGGCTG
GTGAGTGAGAAGCTTGTACGGCAGCATAATGCCCTCCCTCTATGGAGGCGTGGTAACAAGCTGTTTATCGGTATATCGGA
CCCGACAAACCATCAGGCGATCACAGACATTCAGTTCAGCACAGGTCTGAATACTGAAGCGATCCTGGTTGAAGATGACA
AGCTGACCGTTGCGATAGACCGTTTTTTCGACACTGAAAGCGGCCTTGGGGAGTTAGCTGACATTGATCTGGGTCTTGAA
ATTGAGCCTGCCAGTGACAAGGAAACCTCGCTTGCAACTCAGAGTGACGCCGATGACGCTCCAGTGGTGCGTTTCGTCAA
CAAGATGCTGATGGACGCCATAAGGCTCGGTTCTTCCGACCTCCATTTTGAACCCTACGAAAAGATTTTTCGCGTTCGCC
TGCGCACCGACGGTATTTTGCATGAAGTTGCCAAACCACCGATCCATCTGGCCAACCGAATTGCAGCGCGCTTGAAAGTC
ATGGCAAGCCTTGATATCTCTGAGCGGCGCAAACCGCAGGACGGCAGGGTCAAGCTGCGAGTGTCCAAAAATAAAGCGAT
CGACTTCCGTATGAATACGCTGCCAACACTGTGGGGCGAAAAGATCGTGATGCGGATTCTCGATCCGACCAGCGCACAGA
TGGGCATTGACGCATTGGGCTATGAGCCGGACCAAAAAGCTTTGTATATGGAAGCCTTGAAACAACCACAAGGCATGATT
CTTGTAACCGGTCCCACCGGCTCGGGTAAAACCGTTTCGCTATACACCGGCCTGAACATTCTCAACACTGTGGACATCAA
CATTTCCACTGCTGAAGACCCAGTGGAAATCAACCTGGAAGGCATTAATCAGGTAAACGTCAATCCGCGCCAGGGACTGG
ACTTTTCTCAGGCATTGCGCGCATTCCTGCGTCAAGATCCCGACGTAATCATGGTCGGCGAGATACGAGATCTGGAAACC
GCCGAAATCGCCATCAAAGCCTCGCAGACCGGTCACATGGTATTATCTACCCTGCACACTAACAGTGCCGCAGAAACCCT
GACGCGCTTGCATCATATGGGTGTCGCAGCGTTCAACATTGCTACAGCAATCAACCTCATCATTGCCCAGCGGTTGGCGC
GAAAGCTCTGTAGCCATTGCAAGAAAGAACTCGATATACCTCGTGAAACCCTCATCCAGGAGGGCTTTCCGGAAGAGAAG
GTCGGCACGTTCAAAATCTATGGCCCAGTAGGTTGTGAGCACTGCAATGGCGGCTACAGGGGCAGGGTCGGAATTTACGA
AGTGGTCAAGAAAACTCCAGAGTTGGAACGTATTATCATGGAAGAAGGTAATTCGCTGGAGATTTCCAGGCAGATGCGTA
AAGACGGCTTCAATGACTTGAGGACCTCCGGTCTGTCGAAGGCCATGCAGGGCATTACCAGCCTCGAAGAAGTCAACCGT
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.688

100

0.587
  pilB Acinetobacter baylyi ADP1

57.473

99.645

0.573
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.56
  pilB Vibrio cholerae strain A1552

50.98

99.468

0.507
  pilB Vibrio campbellii strain DS40M4

49.291

100

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

50.545

97.518

0.493
  pilF Neisseria gonorrhoeae MS11

48.307

99.468

0.481
  pilF Thermus thermophilus HB27

39.399

100

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.089

93.44

0.365

Multiple sequence alignment