Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH36_RS12690 Genome accession   NZ_AP027939
Coordinates   2784499..2785638 (+) Length   379 a.a.
NCBI ID   WP_026457954.1    Uniprot ID   -
Organism   Aeromonas enteropelogenes strain VA111     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2779499..2790638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH36_RS12660 (VAWG006_24530) - 2779689..2780069 (-) 381 WP_338610404.1 diacylglycerol kinase -
  AACH36_RS12665 (VAWG006_24540) - 2780213..2781274 (+) 1062 WP_026456871.1 3-deoxy-7-phosphoheptulonate synthase -
  AACH36_RS12670 (VAWG006_24550) - 2781355..2782140 (-) 786 WP_225631846.1 ATP-binding cassette domain-containing protein -
  AACH36_RS12675 (VAWG006_24560) btuC 2782127..2783134 (-) 1008 WP_338610406.1 vitamin B12 ABC transporter permease BtuC -
  AACH36_RS12680 (VAWG006_24570) - 2783237..2783968 (-) 732 WP_338610408.1 transporter substrate-binding domain-containing protein -
  AACH36_RS12685 (VAWG006_24580) - 2784083..2784439 (+) 357 WP_005337656.1 DMT family protein -
  AACH36_RS12690 (VAWG006_24590) pilU 2784499..2785638 (+) 1140 WP_026457954.1 type IVa pilus ATPase TapW Machinery gene
  AACH36_RS12695 (VAWG006_24600) - 2785709..2786158 (-) 450 WP_042028581.1 isoprenylcysteine carboxylmethyltransferase family protein -
  AACH36_RS12700 (VAWG006_24610) alr 2786321..2787547 (+) 1227 WP_061475732.1 alanine racemase -
  AACH36_RS12705 (VAWG006_24620) - 2787741..2788685 (-) 945 WP_061475733.1 TerC family protein -
  AACH36_RS12710 (VAWG006_24630) oppF 2788983..2789978 (-) 996 WP_042028578.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42308.37 Da        Isoelectric Point: 6.4287

>NTDB_id=103001 AACH36_RS12690 WP_026457954.1 2784499..2785638(+) (pilU) [Aeromonas enteropelogenes strain VA111]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGVLRPLTETPLNPGDVASIADAIMDKEQKEQFERELEMNLAISLPQVGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWREKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=103001 AACH36_RS12690 WP_026457954.1 2784499..2785638(+) (pilU) [Aeromonas enteropelogenes strain VA111]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTTTATCTGTCAACCGGCGCTCCCCCCTG
CGCCAAGTTCAACGGGGTGTTGCGCCCGCTGACCGAGACGCCCCTCAATCCGGGAGATGTGGCGAGCATCGCCGACGCCA
TCATGGACAAGGAGCAGAAGGAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGGTAGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGTAACGAAGTCTCCCTGGTGGCACGCAACATCAAAACCGAGATCCCGCGCTTTGA
AGATCTCAAACTCCCCCCCGTGCTGCTCGACACCATCATGGAAAAACGGGGCCTGGTGCTGTTCGTCGGGGGTACCGGCT
CGGGCAAATCCACCTCACTGGCGGCCCTTATCGATCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATTGAGGAT
CCGGTAGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTCGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGGGAAATTCGTGATCGCGAGACCATGGAGCATG
CCCTGGCCTTCTCGGAAACCGGTCACCTCGCCATCTCCACCCTGCACGCCAACAACGCTAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGGCGCCCGCAATTGCTCAACGATCTCGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GTTGGTGAAGACCACGGACGGCGGTCGCCGGGCGGCGGTGGAGATCATGCTTGGCACTCACACCATACGTGACATGGTCA
AACGGGGTGAATTTGGCGGTCTGAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGATAGCGCCCTG
TTCGATCTGGTAGTGGAAGGGGTGATCGATGAAGAAGAAGCGGTGAAGAACGCCGACTCGGCCAATAACCTGCGCCTCAA
GATCAAGTTGTGGCGGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAAGACGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.6

98.945

0.53
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

50.142

93.14

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Legionella pneumophila strain ERS1305867

41.617

88.127

0.367
  pilT Legionella pneumophila strain Lp02

41.617

88.127

0.367
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364

Multiple sequence alignment