Detailed information NTG1027298

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB4P91_RS17595
• Genome accession: NZ_CP162590
• Coordinates: 3940111..3941217 (+)
• Length: 368 a.a.
• NCBI ID: WP_395603314.1
• Uniprot ID: -
• Organism: Pseudomonas sp. B21128
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3935111..3946217

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB4P91_RS17580 (AB4P91_17545)	-	3936044..3936976 (+)	933	WP_150687177.1	sugar kinase	-
AB4P91_RS17585 (AB4P91_17550)	-	3937119..3937394 (-)	276	WP_395603312.1	peptidylprolyl isomerase	-
AB4P91_RS17590 (AB4P91_17555)	-	3937446..3939983 (-)	2538	WP_395603313.1	PAS domain-containing protein	-
AB4P91_RS17595 (AB4P91_17560)	pilU	3940111..3941217 (+)	1107	WP_395603314.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB4P91_RS17600 (AB4P91_17565)	-	3941223..3943178 (-)	1956	WP_395603315.1	acetoacetate--CoA ligase	-
AB4P91_RS17605 (AB4P91_17570)	hbdH	3943326..3944099 (-)	774	WP_150592048.1	3-hydroxybutyrate dehydrogenase	-
AB4P91_RS17610 (AB4P91_17575)	-	3944107..3945498 (-)	1392	WP_150592047.1	GntP family permease	-

Sequence

Protein

• Length: 368 a.a.
• Molecular weight: 40712.63 Da
• Isoelectric Point: 6.6992

>NTDB_id=1027298 AB4P91_RS17595 WP_395603314.1 3940111..3941217(+) (pilU) [Pseudomonas sp. B21128]
MEIDALLKQLASRHGSDLFLSTGAPPSARFEGVLTPLSEQPFKPGEVATVASSLMDAEQRREFDRDLEMNLAISRPGTGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLVLFVGATDSGKSTSLAALIDHRNRHSSGHIVTIED
PIEYIHRHRLSIINQREVGVDTRSFQAALKNTLRQAPDVVLIGEIRDRETMEHALAFAETGHLVLSTLHANNAHQALDRI
INLFAEERRPQLLHALGNNLKAFVSQRLVRTLDGQRRAAVEVMLGTPTIADLIRRNELGELKGIMEKSAELGMQTFDAAL
HALVVEGAISEDEALKHADSVNNLRLRLKLQADAVAAPSASGEWGLMD

Nucleotide

• Length: 1107 bp

>NTDB_id=1027298 AB4P91_RS17595 WP_395603314.1 3940111..3941217(+) (pilU) [Pseudomonas sp. B21128]
ATGGAAATCGATGCACTGCTAAAACAACTGGCGAGTCGTCATGGTTCGGACCTGTTCCTCTCCACGGGCGCTCCGCCCAG
TGCACGTTTTGAAGGCGTGCTTACCCCGTTGAGCGAGCAACCTTTCAAACCCGGCGAGGTGGCAACGGTAGCCTCCTCCC
TCATGGACGCCGAGCAGCGCAGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCAATCTCACGCCCCGGCACCGGTCGC
TTCCGGGTCAACATCTTTAAACAGCGCAACGATGTATCCATTGTGATCCGCAACGTCAAACTCGATATTCCACGTTTCGA
GGATCTCAAGCTGCCGCCAGTATTGCTCGAAACCGTGATGCTCAAGCAGGGGCTGGTACTGTTTGTCGGCGCCACCGATT
CGGGAAAATCAACCTCGCTGGCAGCACTCATCGATCACCGCAACCGCCACAGCAGCGGACATATCGTCACCATCGAAGAC
CCGATCGAGTACATCCACCGGCACCGGCTTTCGATCATCAACCAGCGTGAAGTCGGAGTCGACACGCGCAGCTTTCAAGC
CGCGCTGAAAAATACCCTGCGCCAGGCGCCCGACGTGGTACTGATCGGGGAAATCCGTGACCGCGAAACCATGGAACATG
CGCTGGCATTTGCCGAAACCGGTCACTTGGTGCTGTCGACCCTGCACGCCAACAATGCCCATCAGGCACTCGATCGGATC
ATCAACCTGTTTGCCGAGGAGCGCCGACCGCAGTTACTGCATGCCTTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
CTTGGTCCGCACGCTCGACGGGCAACGGCGTGCGGCGGTCGAAGTGATGCTGGGCACGCCCACCATTGCCGATCTGATCC
GGCGTAACGAATTGGGCGAACTCAAAGGCATCATGGAGAAGTCCGCTGAGTTGGGCATGCAGACCTTTGACGCGGCGCTG
CACGCCCTGGTGGTGGAAGGGGCCATCAGCGAAGACGAAGCGCTGAAACATGCCGACTCGGTGAACAACCTGCGCCTGCG
TTTGAAGTTGCAAGCGGATGCGGTTGCGGCGCCATCCGCGTCGGGTGAGTGGGGCCTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (76-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.586	99.728	0.554
pilU	Acinetobacter baylyi ADP1	53.933	96.739	0.522
pilU	Vibrio cholerae strain A1552	52	95.109	0.495
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.413	92.12	0.372
pilT	Pseudomonas stutzeri DSM 10701	39.244	93.478	0.367
pilT	Vibrio cholerae strain A1552	40.24	90.489	0.364
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.24	90.489	0.364
pilT	Pseudomonas aeruginosa PAK	38.953	93.478	0.364
pilT	Legionella pneumophila strain Lp02	39.82	90.761	0.361
pilT	Legionella pneumophila strain ERS1305867	39.82	90.761	0.361