Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB3X10_RS06980 Genome accession   NZ_CP162487
Coordinates   1665629..1666759 (+) Length   376 a.a.
NCBI ID   WP_145701956.1    Uniprot ID   -
Organism   Xanthomonas bundabergensis strain DAR 80977     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1660629..1671759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3X10_RS06955 (AB3X10_06945) - 1660915..1661370 (-) 456 WP_369980225.1 DUF4426 domain-containing protein -
  AB3X10_RS06960 (AB3X10_06950) proC 1661384..1662217 (-) 834 WP_369980227.1 pyrroline-5-carboxylate reductase -
  AB3X10_RS06965 (AB3X10_06955) - 1662387..1663433 (-) 1047 WP_369980229.1 hypothetical protein -
  AB3X10_RS06970 (AB3X10_06960) - 1663712..1664353 (-) 642 WP_369981704.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB3X10_RS06975 (AB3X10_06965) pilT 1664478..1665515 (+) 1038 WP_104557606.1 type IV pilus twitching motility protein PilT Machinery gene
  AB3X10_RS06980 (AB3X10_06970) pilU 1665629..1666759 (+) 1131 WP_145701956.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB3X10_RS06985 (AB3X10_06975) - 1666832..1671262 (+) 4431 WP_369980231.1 RHS repeat-associated core domain-containing protein -
  AB3X10_RS06990 (AB3X10_06980) - 1671283..1671564 (+) 282 WP_369980232.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41942.24 Da        Isoelectric Point: 6.8811

>NTDB_id=1026417 AB3X10_RS06980 WP_145701956.1 1665629..1666759(+) (pilU) [Xanthomonas bundabergensis strain DAR 80977]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTSQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1026417 AB3X10_RS06980 WP_145701956.1 1665629..1666759(+) (pilU) [Xanthomonas bundabergensis strain DAR 80977]
ATGAGCACCATCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCTCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGT
GTGGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATCCC
CACCGTCGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCCATCTCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTGAACCTGCGCGGCGTGGT
CGCGCAGCAGCTGATCCCGACCCCCGACGGCAAGGGCCGGCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGATTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAGGACGCTGGCGCAGGGCATGGACGGGGTGGAGATCGCGG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.854

98.138

0.646
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362

Multiple sequence alignment