Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB3F25_RS07015 Genome accession   NZ_CP162010
Coordinates   1512825..1514222 (+) Length   465 a.a.
NCBI ID   WP_373603145.1    Uniprot ID   -
Organism   Aggregatibacter sp. HMT-949 strain W10649     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1507825..1519222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3F25_RS06985 (AB3F25_06985) - 1508104..1508955 (-) 852 WP_373603140.1 thymidylate synthase -
  AB3F25_RS06990 (AB3F25_06990) lgt 1508965..1509783 (-) 819 WP_373603141.1 prolipoprotein diacylglyceryl transferase -
  AB3F25_RS06995 (AB3F25_06995) - 1509792..1510589 (-) 798 WP_373603142.1 sulfite exporter TauE/SafE family protein -
  AB3F25_RS07000 (AB3F25_07000) rppH 1510709..1511177 (-) 469 Protein_1338 RNA pyrophosphohydrolase -
  AB3F25_RS07005 (AB3F25_07005) ampD 1511703..1512266 (-) 564 WP_373603143.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB3F25_RS07010 (AB3F25_07010) pilA 1512391..1512828 (+) 438 WP_373603144.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AB3F25_RS07015 (AB3F25_07015) pilB 1512825..1514222 (+) 1398 WP_373603145.1 GspE/PulE family protein Machinery gene
  AB3F25_RS07020 (AB3F25_07020) pilC 1514237..1515457 (+) 1221 WP_373603146.1 type II secretion system F family protein Machinery gene
  AB3F25_RS07025 (AB3F25_07025) pilD 1515454..1516140 (+) 687 WP_373603147.1 prepilin peptidase Machinery gene
  AB3F25_RS07030 (AB3F25_07030) coaE 1516372..1516995 (+) 624 WP_373603148.1 dephospho-CoA kinase -
  AB3F25_RS07035 (AB3F25_07035) yacG 1516985..1517191 (+) 207 WP_373603149.1 DNA gyrase inhibitor YacG -
  AB3F25_RS07040 (AB3F25_07040) - 1517344..1518348 (+) 1005 WP_373603150.1 ornithine carbamoyltransferase -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 52614.77 Da        Isoelectric Point: 5.2019

>NTDB_id=1023490 AB3F25_RS07015 WP_373603145.1 1512825..1514222(+) (pilB) [Aggregatibacter sp. HMT-949 strain W10649]
MTTYYLTTVTAQNGEIFEISPELWARNQQQSALFLRYFTLPLKEEEHRIWLAVDSLNNLSACETIAFICGKLVEPVLLDS
VQLKALLQQLAPQASQEEEQVNFYASQEEESTDSLDDEPVIQLLNRIFESALQKNASDIHLETLQHQFQVRFRIDGVLQP
QTPVSKNLANRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKVVLRAQQNKPVELSFAELGMT
EAQQASFQQALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLRSF
LRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIKQHEIESSLLLVIAQRLVRKRCQKCRQ
SSDVPFIQACDCHQGYHGRIGVYQFLHWQQNGYQTDFDNLRQSGLEKVKQGVTDEAEIHRVLGSA

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=1023490 AB3F25_RS07015 WP_373603145.1 1512825..1514222(+) (pilB) [Aggregatibacter sp. HMT-949 strain W10649]
ATGACAACTTATTATTTAACAACGGTTACCGCACAAAACGGCGAAATCTTCGAGATTTCGCCTGAACTTTGGGCTCGAAA
CCAACAACAAAGTGCGCTGTTTTTACGCTATTTTACCCTCCCCTTAAAAGAAGAAGAGCATCGAATCTGGCTGGCGGTCG
ATTCTCTAAATAATCTCTCCGCCTGCGAAACCATCGCCTTTATTTGCGGCAAATTGGTAGAACCGGTATTGCTCGACAGC
GTACAACTTAAAGCGCTTTTGCAACAACTAGCACCACAAGCTTCACAAGAGGAAGAACAGGTTAACTTTTACGCCTCGCA
AGAAGAGGAATCGACCGATTCGTTGGATGATGAACCGGTCATTCAATTGCTCAATCGTATCTTTGAATCCGCTCTGCAAA
AGAACGCGTCCGATATTCATTTGGAAACCTTACAACATCAATTCCAAGTGCGGTTCCGAATTGATGGAGTATTGCAACCA
CAAACTCCGGTCAGTAAAAATCTGGCGAATCGTTTAATCTCTCGCTTAAAATTACTTGCTAAACTCGACATCAGTGAAAC
CCGCCTACCACAAGACGGCCGTTTTCAGTTTAAAACCACATTTTCCGATATTCTTGATTTTCGTCTTTCCACTTTGCCAA
CCCATTGGGGTGAAAAAGTGGTACTGCGCGCCCAACAAAACAAACCGGTCGAACTCAGCTTTGCCGAATTGGGTATGACG
GAAGCCCAACAAGCTTCGTTCCAACAGGCACTCAGCCAACCGCAGGGATTAATTTTAGTCACCGGACCGACCGGCAGCGG
AAAAAGTATTTCACTTTACACTGCACTTCAATGGCTTAATACGCCCGATAAACACATTATGACGGCGGAGGATCCGATAG
AAATTGAACTTGACGGCATTATTCAAAGCCAAATCAATCCGCAAATTGGACTGGATTTTAGCCGACTTTTACGTTCTTTT
TTGCGTCAAGATCCCGATATCATTATGCTTGGCGAAATTCGTGATGAAGAAAGCGCCGCCATGGCGTTACGCGCCGCACA
AACCGGACATCTGGTACTTTCCACCCTTCATACCAATGATGCGATTTCCGCGATTTCTCGTTTACAACAACTCGGCATCA
AGCAACACGAGATCGAAAGCAGCTTACTACTCGTCATCGCCCAGCGACTAGTACGCAAGCGTTGCCAAAAGTGCAGACAA
TCTTCAGATGTTCCCTTCATCCAGGCTTGTGATTGTCATCAAGGTTATCACGGACGCATTGGTGTTTACCAATTCTTACA
TTGGCAACAAAACGGTTATCAAACGGATTTCGACAATCTTCGTCAAAGCGGATTGGAAAAAGTAAAACAAGGCGTAACGG
ACGAAGCAGAAATCCATCGCGTTTTAGGCTCGGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(126-393)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

82.618

100

0.828
  pilB Haemophilus influenzae Rd KW20

80.901

100

0.811
  pilB Glaesserella parasuis strain SC1401

57.203

100

0.581
  pilB Vibrio campbellii strain DS40M4

41.736

100

0.434
  pilB Vibrio cholerae strain A1552

41.237

100

0.43
  pilB Acinetobacter baumannii D1279779

41.336

100

0.426
  pilB Acinetobacter baylyi ADP1

39.437

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

40.501

100

0.417
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.413
  pilF Neisseria gonorrhoeae MS11

38.285

100

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.583

100

0.374
  pilF Thermus thermophilus HB27

35.745

100

0.361

Multiple sequence alignment