Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AB3F25_RS07010 Genome accession   NZ_CP162010
Coordinates   1512391..1512828 (+) Length   145 a.a.
NCBI ID   WP_373603144.1    Uniprot ID   -
Organism   Aggregatibacter sp. HMT-949 strain W10649     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1507391..1517828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3F25_RS06980 (AB3F25_06980) tadA 1507564..1508094 (-) 531 WP_373603139.1 tRNA adenosine(34) deaminase TadA -
  AB3F25_RS06985 (AB3F25_06985) - 1508104..1508955 (-) 852 WP_373603140.1 thymidylate synthase -
  AB3F25_RS06990 (AB3F25_06990) lgt 1508965..1509783 (-) 819 WP_373603141.1 prolipoprotein diacylglyceryl transferase -
  AB3F25_RS06995 (AB3F25_06995) - 1509792..1510589 (-) 798 WP_373603142.1 sulfite exporter TauE/SafE family protein -
  AB3F25_RS07000 (AB3F25_07000) rppH 1510709..1511177 (-) 469 Protein_1338 RNA pyrophosphohydrolase -
  AB3F25_RS07005 (AB3F25_07005) ampD 1511703..1512266 (-) 564 WP_373603143.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB3F25_RS07010 (AB3F25_07010) pilA 1512391..1512828 (+) 438 WP_373603144.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AB3F25_RS07015 (AB3F25_07015) pilB 1512825..1514222 (+) 1398 WP_373603145.1 GspE/PulE family protein Machinery gene
  AB3F25_RS07020 (AB3F25_07020) pilC 1514237..1515457 (+) 1221 WP_373603146.1 type II secretion system F family protein Machinery gene
  AB3F25_RS07025 (AB3F25_07025) pilD 1515454..1516140 (+) 687 WP_373603147.1 prepilin peptidase Machinery gene
  AB3F25_RS07030 (AB3F25_07030) coaE 1516372..1516995 (+) 624 WP_373603148.1 dephospho-CoA kinase -
  AB3F25_RS07035 (AB3F25_07035) yacG 1516985..1517191 (+) 207 WP_373603149.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15014.34 Da        Isoelectric Point: 8.7944

>NTDB_id=1023489 AB3F25_RS07010 WP_373603144.1 1512391..1512828(+) (pilA) [Aggregatibacter sp. HMT-949 strain W10649]
MKFISSHVLKKGFTLIELMIVIAIIAILATIAIPSYQNYTQKAAMSELLQASAPYKADVELCIYGTGTTTNCSGGSNGVA
ANTTAAKGYIKSISTNAGAITVVGDGTLKDIQYTLTATGDKSKGITWAASCTGDKSLFPAGFCSK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1023489 AB3F25_RS07010 WP_373603144.1 1512391..1512828(+) (pilA) [Aggregatibacter sp. HMT-949 strain W10649]
ATGAAATTTATTTCTTCACACGTATTAAAAAAGGGTTTTACTCTAATTGAATTAATGATTGTCATCGCGATTATCGCCAT
TTTGGCAACTATTGCCATTCCCTCTTATCAAAATTACACCCAAAAAGCGGCAATGTCCGAATTATTGCAAGCTTCCGCGC
CTTATAAAGCAGATGTTGAGCTGTGCATTTACGGTACTGGCACCACCACTAATTGTAGCGGCGGGAGTAACGGTGTGGCG
GCCAATACCACTGCTGCAAAAGGTTATATAAAAAGTATCAGTACTAACGCCGGTGCGATTACTGTGGTTGGTGACGGCAC
ATTGAAAGACATTCAATACACGCTCACCGCCACTGGTGACAAAAGTAAGGGCATAACTTGGGCGGCCAGCTGTACCGGTG
ATAAATCTCTCTTCCCGGCCGGATTCTGTTCTAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

70.833

99.31

0.703
  pilA Haemophilus influenzae Rd KW20

66.667

99.31

0.662
  pilA Glaesserella parasuis strain SC1401

59.155

97.931

0.579
  comP Acinetobacter baylyi ADP1

41.135

97.241

0.4
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.838

100

0.386
  pilA Pseudomonas aeruginosa PAK

34.194

100

0.366
  pilA Vibrio campbellii strain DS40M4

36.301

100

0.366

Multiple sequence alignment