Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   U2F28_RS04150 Genome accession   NZ_CP160635
Coordinates   858453..858881 (+) Length   142 a.a.
NCBI ID   WP_064305810.1    Uniprot ID   -
Organism   Staphylococcus aureus strain BSN44     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 849096..899186 858453..858881 within 0


Gene organization within MGE regions


Location: 849096..899186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2F28_RS04065 (U2F28_004065) sufB 849096..850493 (+) 1398 WP_001074405.1 Fe-S cluster assembly protein SufB -
  U2F28_RS04070 (U2F28_004070) - 850561..851610 (-) 1050 WP_001145726.1 tyrosine-type recombinase/integrase -
  U2F28_RS04075 (U2F28_004075) - 851723..851902 (+) 180 WP_000337826.1 hypothetical protein -
  U2F28_RS04080 (U2F28_004080) - 851882..852814 (-) 933 WP_000392186.1 hypothetical protein -
  U2F28_RS04085 (U2F28_004085) - 852846..853571 (-) 726 WP_000661437.1 PH domain-containing protein -
  U2F28_RS04090 (U2F28_004090) - 853599..854273 (-) 675 WP_322411404.1 ImmA/IrrE family metallo-endopeptidase -
  U2F28_RS04095 (U2F28_004095) - 854290..854622 (-) 333 WP_001055143.1 helix-turn-helix domain-containing protein -
  U2F28_RS04100 (U2F28_004100) - 854885..855079 (+) 195 WP_000108122.1 helix-turn-helix transcriptional regulator -
  U2F28_RS04105 (U2F28_004105) - 855079..855843 (+) 765 WP_064305808.1 phage antirepressor KilAC domain-containing protein -
  U2F28_RS04110 (U2F28_004110) - 855860..856054 (+) 195 WP_001148857.1 hypothetical protein -
  U2F28_RS04115 (U2F28_004115) - 856085..856228 (+) 144 WP_000939503.1 hypothetical protein -
  U2F28_RS04120 (U2F28_004120) - 856249..856806 (-) 558 WP_000388992.1 hypothetical protein -
  U2F28_RS04125 (U2F28_004125) - 856877..857098 (+) 222 WP_064305809.1 hypothetical protein -
  U2F28_RS04130 (U2F28_004130) - 857091..857252 (+) 162 WP_052995610.1 DUF1270 family protein -
  U2F28_RS04135 (U2F28_004135) - 857346..857606 (+) 261 WP_000291090.1 DUF1108 family protein -
  U2F28_RS04140 (U2F28_004140) - 857616..857837 (+) 222 WP_000815401.1 DUF2483 family protein -
  U2F28_RS04145 (U2F28_004145) - 857830..858453 (+) 624 WP_000139720.1 DUF1071 domain-containing protein -
  U2F28_RS04150 (U2F28_004150) ssbA 858453..858881 (+) 429 WP_064305810.1 single-stranded DNA-binding protein Machinery gene
  U2F28_RS04155 (U2F28_004155) - 858895..859563 (+) 669 WP_064305811.1 putative HNHc nuclease -
  U2F28_RS04160 (U2F28_004160) - 859563..860345 (+) 783 WP_017804645.1 AP2 domain-containing protein -
  U2F28_RS04165 (U2F28_004165) - 860317..861120 (+) 804 WP_000504985.1 phage replisome organizer N-terminal domain-containing protein -
  U2F28_RS04170 (U2F28_004170) - 861130..861909 (+) 780 WP_000803070.1 ATP-binding protein -
  U2F28_RS04175 (U2F28_004175) - 861903..862061 (+) 159 WP_000256597.1 hypothetical protein -
  U2F28_RS04180 (U2F28_004180) - 862075..862296 (+) 222 WP_064305812.1 DUF3269 family protein -
  U2F28_RS04185 (U2F28_004185) - 862306..862713 (+) 408 WP_064305813.1 RusA family crossover junction endodeoxyribonuclease -
  U2F28_RS04190 (U2F28_004190) - 862713..862898 (+) 186 WP_031927496.1 DUF3113 family protein -
  U2F28_RS04195 (U2F28_004195) - 862899..863516 (+) 618 WP_064305814.1 SA1788 family PVL leukocidin-associated protein -
  U2F28_RS04200 (U2F28_004200) - 863517..863765 (+) 249 WP_001126836.1 phi PVL orf 51-like protein -
  U2F28_RS04205 (U2F28_004205) - 863780..864031 (+) 252 WP_001065046.1 DUF1024 family protein -
  U2F28_RS04210 (U2F28_004210) - 864021..864194 (+) 174 WP_000028424.1 hypothetical protein -
  U2F28_RS04215 (U2F28_004215) - 864195..864356 (+) 162 WP_000889680.1 hypothetical protein -
  U2F28_RS04220 (U2F28_004220) - 864371..864904 (+) 534 WP_061839007.1 dUTPase -
  U2F28_RS04225 (U2F28_004225) - 864941..865114 (+) 174 WP_001209217.1 hypothetical protein -
  U2F28_RS04230 (U2F28_004230) - 865131..865337 (+) 207 WP_015988673.1 DUF1381 domain-containing protein -
  U2F28_RS04235 (U2F28_004235) - 865334..865537 (+) 204 WP_015988674.1 hypothetical protein -
  U2F28_RS04240 (U2F28_004240) - 865530..865766 (+) 237 WP_000608283.1 hypothetical protein -
  U2F28_RS04245 (U2F28_004245) - 865756..866145 (+) 390 WP_001596346.1 hypothetical protein -
  U2F28_RS04250 (U2F28_004250) - 866142..866315 (+) 174 WP_000595257.1 transcriptional activator RinB -
  U2F28_RS04255 (U2F28_004255) - 866316..866462 (+) 147 WP_000989998.1 hypothetical protein -
  U2F28_RS04260 (U2F28_004260) - 866486..866908 (+) 423 WP_000162701.1 RinA family phage transcriptional activator -
  U2F28_RS04265 (U2F28_004265) - 867239..867733 (+) 495 WP_000594087.1 terminase small subunit -
  U2F28_RS04270 (U2F28_004270) - 867726..868949 (+) 1224 WP_064305815.1 PBSX family phage terminase large subunit -
  U2F28_RS04275 (U2F28_004275) - 868946..870370 (+) 1425 WP_000177422.1 phage portal protein -
  U2F28_RS04280 (U2F28_004280) - 870339..871292 (+) 954 WP_000184133.1 phage head morphogenesis protein -
  U2F28_RS04285 (U2F28_004285) - 871294..871500 (+) 207 WP_000346033.1 hypothetical protein -
  U2F28_RS04290 (U2F28_004290) - 871603..872187 (+) 585 WP_001019219.1 DUF4355 domain-containing protein -
  U2F28_RS04295 (U2F28_004295) - 872204..873118 (+) 915 WP_000235168.1 phage major capsid protein -
  U2F28_RS04300 (U2F28_004300) - 873130..873273 (+) 144 WP_000002931.1 hypothetical protein -
  U2F28_RS04305 (U2F28_004305) - 873279..873629 (+) 351 WP_000177351.1 phage head-tail adapter protein -
  U2F28_RS04310 (U2F28_004310) - 873641..873976 (+) 336 WP_000483041.1 phage head closure protein -
  U2F28_RS04315 (U2F28_004315) - 873963..874376 (+) 414 WP_001151330.1 HK97-gp10 family putative phage morphogenesis protein -
  U2F28_RS04320 (U2F28_004320) - 874389..874814 (+) 426 WP_000270192.1 DUF3168 domain-containing protein -
  U2F28_RS04325 (U2F28_004325) - 874815..875372 (+) 558 WP_000057582.1 tail protein -
  U2F28_RS04330 (U2F28_004330) - 875439..875945 (+) 507 WP_000134337.1 tail assembly chaperone -
  U2F28_RS04335 (U2F28_004335) - 875990..876274 (+) 285 WP_000880587.1 hypothetical protein -
  U2F28_RS04340 (U2F28_004340) - 876278..879163 (+) 2886 WP_017431803.1 hypothetical protein -
  U2F28_RS04345 (U2F28_004345) - 879178..880119 (+) 942 WP_064305816.1 phage tail domain-containing protein -
  U2F28_RS04350 (U2F28_004350) - 880130..882016 (+) 1887 WP_001122001.1 SGNH/GDSL hydrolase family protein -
  U2F28_RS04355 (U2F28_004355) - 882029..883927 (+) 1899 WP_322411405.1 hypothetical protein -
  U2F28_RS04360 (U2F28_004360) - 883927..885750 (+) 1824 WP_064305817.1 BppU family phage baseplate upper protein -
  U2F28_RS04365 (U2F28_004365) - 885750..886127 (+) 378 WP_061644684.1 DUF2977 domain-containing protein -
  U2F28_RS04370 (U2F28_004370) - 886131..886304 (+) 174 WP_001790193.1 XkdX family protein -
  U2F28_RS04375 (U2F28_004375) - 886345..886644 (+) 300 WP_000466769.1 DUF2951 domain-containing protein -
  U2F28_RS04380 (U2F28_004380) - 886781..888655 (+) 1875 WP_064305818.1 glucosaminidase domain-containing protein -
  U2F28_RS04385 (U2F28_004385) - 888668..889840 (+) 1173 WP_053507572.1 BppU family phage baseplate upper protein -
  U2F28_RS04390 (U2F28_004390) - 889846..890241 (+) 396 WP_015978410.1 hypothetical protein -
  U2F28_RS04395 (U2F28_004395) - 890297..890572 (+) 276 WP_000351119.1 phage holin -
  U2F28_RS04400 (U2F28_004400) - 890559..891971 (+) 1413 WP_001141517.1 N-acetylmuramoyl-L-alanine amidase -
  U2F28_RS04405 (U2F28_004405) - 892031..892588 (-) 558 WP_001035620.1 DUF4888 domain-containing protein -
  U2F28_RS04410 (U2F28_004410) - 892963..893115 (+) 153 WP_001788502.1 hypothetical protein -
  U2F28_RS04415 (U2F28_004415) - 893186..893296 (+) 111 WP_000139423.1 hypothetical protein -
  U2F28_RS04420 (U2F28_004420) - 893298..893483 (+) 186 WP_001286805.1 hypothetical protein -
  U2F28_RS04425 (U2F28_004425) - 894168..894307 (+) 140 Protein_863 hypothetical protein -
  U2F28_RS04430 (U2F28_004430) - 894313..894627 (-) 315 WP_000274029.1 hypothetical protein -
  U2F28_RS04435 (U2F28_004435) - 894992..896032 (+) 1041 WP_000582326.1 hemolysin family protein -
  U2F28_RS04440 (U2F28_004440) - 896046..897113 (+) 1068 WP_000267236.1 nitronate monooxygenase family protein -
  U2F28_RS04445 (U2F28_004445) - 897367..897450 (+) 84 WP_016170465.1 hypothetical protein -
  U2F28_RS04450 (U2F28_004450) - 897498..898346 (+) 849 WP_000608129.1 DUF72 domain-containing protein -
  U2F28_RS04455 (U2F28_004455) - 898359..899186 (+) 828 WP_000927632.1 sulfite exporter TauE/SafE family protein -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 15874.55 Da        Isoelectric Point: 8.4724

>NTDB_id=1021792 U2F28_RS04150 WP_064305810.1 858453..858881(+) (ssbA) [Staphylococcus aureus strain BSN44]
MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGEREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTR
SYDNKDGKRVFVTEVVADSVQFLEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF

Nucleotide


Download         Length: 429 bp        

>NTDB_id=1021792 U2F28_RS04150 WP_064305810.1 858453..858881(+) (ssbA) [Staphylococcus aureus strain BSN44]
ATGTTAAACAGAGCAGTATTAGTAGGACGCTTAACAAAAGACCCAGAATTAAGAAGCGCGCCAAATGGCGTAAATGTAGG
TACATTCACATTGGCAGTAAACAGAACATTCACGAATGCTCAAGGCGAGCGTGAAGCAGATTTTATAAACGTAGTAGTGT
TCAAGAAACAAGCTGAAAATGTTAAAAACTACCTTTCTAAAGGGTCGCTGGCAGGTGTAGACGGGCGACTACAAACACGT
AGCTACGATAACAAAGACGGGAAACGTGTATTTGTTACAGAAGTAGTAGCGGACAGTGTTCAATTCTTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAAGACAAACTCAAACTGGTAATAATCCTTTTGATAATACCACTG
CGATTACTGATGATGACTTACCGTTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

56.977

100

0.69

  ssb Latilactobacillus sakei subsp. sakei 23K

49.412

100

0.592

  ssbB Bacillus subtilis subsp. subtilis str. 168

57.547

74.648

0.43

  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.259

100

0.415

  ssbA Streptococcus mutans UA159

40.845

100

0.408

  ssbB/cilA Streptococcus mitis NCTC 12261

38.732

100

0.387

  ssbB/cilA Streptococcus pneumoniae TIGR4

38.732

100

0.387

  ssbB/cilA Streptococcus pneumoniae Rx1

38.028

100

0.38

  ssbB/cilA Streptococcus pneumoniae D39

38.028

100

0.38

  ssbB/cilA Streptococcus pneumoniae R6

38.028

100

0.38

  ssbB/cilA Streptococcus mitis SK321

38.028

100

0.38


Multiple sequence alignment