Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AAFM42_RS12280 Genome accession   NZ_CP160329
Coordinates   2437535..2438863 (+) Length   442 a.a.
NCBI ID   WP_053253954.1    Uniprot ID   A0A1E4XNI9
Organism   Pseudomonas fluorescens strain PFX1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2432535..2443863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFM42_RS12260 (AAFM42_12225) - 2432802..2435264 (-) 2463 WP_371850829.1 penicillin-binding protein 1A -
  AAFM42_RS12265 (AAFM42_12230) pilM 2435340..2435996 (+) 657 WP_053254193.1 type IV pilus assembly protein PilM -
  AAFM42_RS12270 (AAFM42_12235) - 2435993..2436544 (+) 552 WP_053253952.1 PilN domain-containing protein -
  AAFM42_RS12275 (AAFM42_12240) - 2436541..2437512 (+) 972 WP_053253953.1 pilus assembly protein PilP -
  AAFM42_RS12280 (AAFM42_12245) pilQ 2437535..2438863 (+) 1329 WP_053253954.1 type IV pilus secretin PilQ Machinery gene
  AAFM42_RS12285 (AAFM42_12250) aroK 2438875..2439393 (+) 519 WP_003209345.1 shikimate kinase AroK -
  AAFM42_RS12290 (AAFM42_12255) aroB 2439432..2440532 (+) 1101 WP_053253955.1 3-dehydroquinate synthase -
  AAFM42_RS12295 (AAFM42_12260) - 2440557..2442125 (+) 1569 WP_053253956.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 442 a.a.        Molecular weight: 48167.26 Da        Isoelectric Point: 4.8928

>NTDB_id=1020516 AAFM42_RS12280 WP_053253954.1 2437535..2438863(+) (pilQ) [Pseudomonas fluorescens strain PFX1]
MAFAVPNRLDLIQLPPPGSSALSTHYSGDKLTLNFQDIALRAVLQQIADVAGLNLVASDDVQGSITLRLKEVPWDQALEL
VLQAKGLDKRVKAGVLLVAPAEELAARELLTLEARKQMAELAPLRRELLQVNYAKAADLAKLFQSVTSLEGIPDERGSVA
VDERTNNIIAYQTGERLEELRRIVAQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGNWGAGGINKPLAEGAEPS
ESLPSSPFVDMGSLTGTSGLGIAYITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSRGATS
VSFKEASLSLEVTPQITPDGWVVMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVP
FLGDVPYLGRLFRRDVLAERKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1329 bp        

>NTDB_id=1020516 AAFM42_RS12280 WP_053253954.1 2437535..2438863(+) (pilQ) [Pseudomonas fluorescens strain PFX1]
ATGGCTTTTGCTGTGCCCAATCGCCTGGACCTGATCCAGCTGCCACCTCCCGGCAGCTCGGCGTTGAGTACCCACTATTC
CGGCGACAAGCTGACCCTCAACTTCCAGGACATCGCTTTGCGCGCTGTATTGCAGCAGATTGCGGATGTCGCCGGGCTTA
ATCTGGTGGCCAGCGACGATGTGCAAGGCTCCATCACCTTGCGGCTCAAGGAGGTGCCCTGGGATCAGGCACTGGAGCTG
GTGTTGCAAGCCAAGGGGTTGGACAAACGTGTGAAAGCCGGTGTGTTGCTGGTGGCGCCGGCCGAAGAGTTGGCCGCACG
CGAACTGCTGACACTGGAGGCGCGCAAGCAAATGGCCGAGTTGGCGCCCTTGCGCCGGGAACTGTTGCAGGTCAATTACG
CCAAGGCGGCGGACCTGGCCAAATTGTTCCAATCGGTCACCAGCCTGGAAGGCATCCCCGATGAGCGCGGTTCGGTCGCC
GTGGATGAACGTACCAACAACATCATCGCCTACCAGACCGGCGAACGGCTCGAAGAGCTGCGGCGGATCGTGGCGCAGCT
GGATGTCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTTGAAGCCAATGTCGATTACGACAAAAGCCTTGGCGTGC
GCTGGGGTGGGCGCCTCAACCGTGGCAATTGGGGGGCGGGCGGTATCAACAAACCGTTGGCCGAGGGCGCTGAACCGTCG
GAAAGCCTGCCCAGCTCGCCGTTTGTCGACATGGGTTCGCTCACCGGTACTTCAGGCCTGGGTATCGCTTATATCACCGA
CAACCTGCTGCTGGACCTTGAGCTGACGGCCATGGAGAAAACCGGCAACGGTGAAATCGTCTCGCAGCCCAAGGTGGTCA
CCTCTGACAAGGAAACCGCGCGGATCCTCAAGGGCACCGAGATCCCTTATCAGGAATCCACCTCGCGGGGTGCCACGTCG
GTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGTGACGCCACAAATCACCCCTGATGGCTGGGTGGTCATGGAGGTCAA
GGTCACCAAGGACGAGCCTGACTATCTGAACAAGCTCAACGACGTGCCGCCGATCAAGAAAAACGAAGTCAACGCCAAGG
TGCTGGTCAAGGATGGCGAGACCATCGTCATCGGTGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCA
TTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAAAGAAAATCCGAGCTGCTGGTATT
CCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(128-193)

Secretin and TonB N terminus short domain (STN)

(54-100)

Bacterial type II and III secretion system protein (Secretin)

(277-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E4XNI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.872

98.643

0.699
  pilQ Acinetobacter baumannii D1279779

40.283

100

0.45
  pilQ Acinetobacter baumannii strain A118

40.283

100

0.45
  comQ Acinetobacter baylyi ADP1

40.71

100

0.441
  pilQ Vibrio campbellii strain DS40M4

44.575

95.928

0.428
  pilQ Legionella pneumophila strain Lp02

43.468

95.249

0.414
  pilQ Vibrio cholerae strain A1552

43.51

94.118

0.41
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.51

94.118

0.41
  pilQ Legionella pneumophila strain ERS1305867

42.993

95.249

0.41
  pilQ Neisseria meningitidis MC58

36.952

97.964

0.362
  pilQ Neisseria meningitidis M1080

36.952

97.964

0.362

Multiple sequence alignment