Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N011_RS14410 Genome accession   NZ_CP159362
Coordinates   3141669..3142886 (-) Length   405 a.a.
NCBI ID   WP_024695105.1    Uniprot ID   A0AAU8LBD3
Organism   Pseudomonas syringae CC1417     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3136669..3147886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N011_RS14375 (N011_14375) - 3137784..3137957 (-) 174 WP_032610814.1 DUF3094 family protein -
  N011_RS14380 (N011_14380) - 3137978..3138511 (-) 534 WP_024696212.1 MOSC domain-containing protein -
  N011_RS14385 (N011_14385) - 3138508..3139137 (-) 630 WP_024685626.1 DUF1780 domain-containing protein -
  N011_RS14390 (N011_14390) - 3139263..3139724 (+) 462 WP_024696213.1 hypothetical protein -
  N011_RS14395 (N011_14395) yacG 3139968..3140177 (-) 210 WP_024684958.1 DNA gyrase inhibitor YacG -
  N011_RS14400 (N011_14400) coaE 3140174..3140797 (-) 624 WP_024684957.1 dephospho-CoA kinase -
  N011_RS14405 (N011_14405) pilD 3140794..3141666 (-) 873 WP_024695104.1 A24 family peptidase Machinery gene
  N011_RS14410 (N011_14410) pilC 3141669..3142886 (-) 1218 WP_024695105.1 type II secretion system F family protein Machinery gene
  N011_RS14415 (N011_14415) pilB 3142889..3144583 (-) 1695 WP_024695106.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N011_RS14420 (N011_14420) - 3144810..3145238 (+) 429 WP_024695107.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N011_RS14430 (N011_14430) - 3145505..3146050 (-) 546 WP_024695108.1 DUF1523 family protein -
  N011_RS14435 (N011_14435) - 3146170..3147168 (+) 999 WP_024695109.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44141.13 Da        Isoelectric Point: 10.2497

>NTDB_id=1013430 N011_RS14410 WP_024695105.1 3141669..3142886(-) (pilC) [Pseudomonas syringae CC1417]
MASKAVKVSVFTWEGVDKKGSKINGELSGHNLALVKAQLRKQGINPTKVRKKAVSIFGNGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIIIDGSENPNMRSLIGTLKQEVSAGNSFATALRKKPEYFDDLFCNLVDAGEQAGALESLLDRVATYKEKT
EKLKAKIKKAMTYPAAVLIVAMIVSGILLIKVVPQFQAIFAGFGAELPAFTLMVIWLSEVMQRWWLVVLGLSVGAFFLFK
NSYRKSGKFRDGIDRFLLKIPVIGPLIFKSSVARYARTLATTFSAGVPLVEALDSVAGATGNVVFRNAVHKIRQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDSMLDKVATYYEEEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1013430 N011_RS14410 WP_024695105.1 3141669..3142886(-) (pilC) [Pseudomonas syringae CC1417]
ATGGCCAGCAAGGCAGTCAAAGTCAGTGTTTTCACCTGGGAAGGCGTCGACAAGAAAGGCAGCAAGATCAACGGTGAACT
GAGCGGCCACAACCTGGCGCTGGTCAAGGCTCAGTTGCGCAAGCAGGGTATTAACCCGACCAAGGTGCGCAAGAAAGCGG
TGTCGATTTTCGGCAACGGTAAAAAGATCAAACCGCTGGATATCGCCTTCTTTTCAAGACAGATGGCGACAATGATGAAA
GCGGGCGTTCCGCTGCTGCAATCCTTCGACATTATTATCGACGGCAGCGAGAACCCCAACATGCGCTCCCTGATCGGCAC
GCTCAAACAAGAGGTTTCGGCAGGCAACAGCTTTGCAACCGCCTTGCGTAAGAAACCGGAATATTTCGATGACCTGTTTT
GTAACCTGGTGGATGCAGGCGAACAGGCAGGTGCCTTGGAAAGCTTGCTCGACCGGGTAGCCACATATAAGGAAAAAACC
GAAAAGCTCAAAGCGAAGATCAAAAAGGCCATGACCTATCCGGCAGCCGTTCTGATTGTCGCGATGATTGTGTCGGGCAT
CCTGCTCATCAAAGTGGTGCCACAATTCCAGGCGATCTTTGCAGGCTTCGGTGCCGAGCTGCCCGCCTTTACCTTGATGG
TCATCTGGCTTTCAGAGGTCATGCAGCGCTGGTGGCTGGTTGTTCTCGGGTTGTCGGTTGGTGCTTTCTTCCTGTTCAAA
AATTCTTATAGAAAATCCGGGAAGTTTCGTGACGGTATCGACAGGTTCCTGCTGAAGATCCCGGTCATTGGCCCACTGAT
TTTCAAGTCATCTGTGGCCCGTTACGCACGAACACTGGCTACCACTTTCTCGGCGGGTGTCCCGTTGGTCGAGGCGCTTG
ATTCAGTTGCTGGTGCCACCGGCAACGTGGTGTTCCGCAACGCGGTCCACAAAATCAGGCAGGACGTATCGACCGGCATG
CAGCTGAATTTCTCGATGCGCTCGACCGGTGTATTCCCCAGCCTTGCCATTCAGATGACGGCCATCGGTGAAGAATCCGG
CGCGCTGGACAGCATGCTCGACAAGGTCGCAACCTATTACGAGGAGGAGGTCGACAACATGGTCGACAGCCTGACCAGCC
TAATGGAGCCGATGATCATGGCCGTACTGGGCGTTATCGTCGGCGGGCTGGTGATCGCCATGTACCTGCCGATCTTCAAA
CTTGGAAATGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.062

100

0.751
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

59.653

99.753

0.595
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

42.68

99.506

0.425
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

40.295

100

0.405
  pilC Vibrio cholerae strain A1552

40.247

100

0.402
  pilC Thermus thermophilus HB27

39.651

99.012

0.393

Multiple sequence alignment