Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N011_RS14415 Genome accession   NZ_CP159362
Coordinates   3142889..3144583 (-) Length   564 a.a.
NCBI ID   WP_024695106.1    Uniprot ID   A0AAU8LBJ6
Organism   Pseudomonas syringae CC1417     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3137889..3149583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N011_RS14380 (N011_14380) - 3137978..3138511 (-) 534 WP_024696212.1 MOSC domain-containing protein -
  N011_RS14385 (N011_14385) - 3138508..3139137 (-) 630 WP_024685626.1 DUF1780 domain-containing protein -
  N011_RS14390 (N011_14390) - 3139263..3139724 (+) 462 WP_024696213.1 hypothetical protein -
  N011_RS14395 (N011_14395) yacG 3139968..3140177 (-) 210 WP_024684958.1 DNA gyrase inhibitor YacG -
  N011_RS14400 (N011_14400) coaE 3140174..3140797 (-) 624 WP_024684957.1 dephospho-CoA kinase -
  N011_RS14405 (N011_14405) pilD 3140794..3141666 (-) 873 WP_024695104.1 A24 family peptidase Machinery gene
  N011_RS14410 (N011_14410) pilC 3141669..3142886 (-) 1218 WP_024695105.1 type II secretion system F family protein Machinery gene
  N011_RS14415 (N011_14415) pilB 3142889..3144583 (-) 1695 WP_024695106.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N011_RS14420 (N011_14420) - 3144810..3145238 (+) 429 WP_024695107.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N011_RS14430 (N011_14430) - 3145505..3146050 (-) 546 WP_024695108.1 DUF1523 family protein -
  N011_RS14435 (N011_14435) - 3146170..3147168 (+) 999 WP_024695109.1 ATP-grasp domain-containing protein -
  N011_RS14440 (N011_14440) - 3147223..3148365 (+) 1143 WP_024695110.1 trypsin-like peptidase domain-containing protein -
  N011_RS14445 (N011_14445) - 3148444..3149577 (+) 1134 WP_024695111.1 phosphoribosyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62259.48 Da        Isoelectric Point: 6.3059

>NTDB_id=1013431 N011_RS14415 WP_024695106.1 3142889..3144583(-) (pilB) [Pseudomonas syringae CC1417]
MSDVVLTGLAKQLVLAELLSEKAAQQANQNAKRDKISLVSYLVQNKLVKSLTLAEIASDQFGVPFMDLASLDKESQPKGL
ISEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLSDAIDKFFDANSGLGDLADVDLGLD
IEISDGKESSLSEQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKVFRVRLRTDGILHEVARPPIHLASRIAARLKV
MASLDISERRKPQDGRVKLRISKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDVPRETLLQEGFPESK
IGTFKIYGPVGCNQCNGGYKGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1013431 N011_RS14415 WP_024695106.1 3142889..3144583(-) (pilB) [Pseudomonas syringae CC1417]
ATGTCTGATGTCGTTCTCACGGGGCTTGCCAAGCAACTGGTGCTGGCCGAGCTTCTTTCTGAAAAAGCTGCACAGCAGGC
TAACCAAAACGCCAAGCGCGACAAAATTTCGTTGGTCAGCTACCTGGTTCAGAACAAGCTGGTCAAAAGTCTGACGCTTG
CCGAGATAGCCTCCGATCAATTCGGTGTGCCGTTCATGGACCTTGCCAGCCTGGACAAGGAAAGTCAGCCCAAAGGGTTG
ATCAGTGAAAAGCTGGTTCGCCAGCACCATGCATTGCCGTTATGGCGTCGGGGCAACAAGCTGTTCATTGGTATCTCGGA
CCCGACCAACCATCAGGCCGTCACCGACATTCAGTTCAGCACCGGCCTCAACACCGAAGCGATCCTGGTAGAGGACGACA
AGCTCAGTGACGCCATCGACAAGTTTTTCGACGCCAACAGCGGTCTGGGCGATCTGGCCGATGTCGACCTCGGGCTGGAC
ATCGAAATCAGCGACGGCAAGGAGTCGTCTCTCAGCGAACAGAGCGATGCCGATGACGCGCCGGTGGTGCGCTTCGTTAA
CAAGATGCTGATGGACGCGATCCGGCTGGGCTCTTCGGACCTGCACTTCGAACCCTACGAGAAAGTATTTCGCGTGCGCC
TGCGTACCGACGGCATCCTGCATGAAGTCGCCCGGCCGCCGATTCACCTCGCCAGCCGAATCGCTGCGCGCTTGAAGGTC
ATGGCCAGCCTGGACATTTCCGAACGACGCAAGCCTCAGGACGGACGGGTCAAGCTGCGCATCTCCAAGACCAAGGCTAT
CGATTTTCGTATGAACACCCTGCCGACGCTGTGGGGTGAGAAGATCGTCATGCGGATTCTCGATCCGACCAGCGCCCAGA
TGGGCATCGATGCCCTGGGCTACGAGCCTGAACAGAAAGCGCTGTATCTGGAAGCGTTGAAGCAGCCACAGGGGATGATT
CTGGTCACAGGCCCCACCGGCTCCGGCAAGACCGTGTCGCTGTACACAGGCCTGAACATCCTCAACACAGTGGATATCAA
CATTTCTACCGCCGAAGACCCGGTCGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGTCAGGGCATGG
ACTTCTCTCAGGCGCTGCGCGCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTGGAAACG
GCAGAAATCGCCATCAAGGCCTCGCAGACCGGTCACATGGTTTTGTCCACGCTGCACACCAACAGCGCGGCGGAAACCCT
GACCCGTTTGCATCATATGGGTGTTGCAGCGTTCAACATTGCCACGGCGATCAACCTGATCATCGCGCAACGTCTGGCTC
GCAAGCTATGCTCCCACTGCAAAAAAGAGCTGGATGTGCCCCGCGAAACACTGCTTCAGGAAGGTTTTCCCGAGTCAAAA
ATAGGCACGTTCAAGATCTACGGCCCGGTCGGCTGTAATCAGTGCAACGGCGGGTACAAGGGGCGGGTCGGCATTTACGA
AGTCGTCAAGAAAACGCCTGAGCTGGAGCGCATCATCATGGAAGAAGGCAATTCGCTGGAGATTTCCAGGCAAATGCGCA
AGGACGGTTTCAATGACCTGCGCACCTCAGGCCTTTCGAAAGCCATGCAGGGCATTACCAGCCTGGAAGAAGTCAATCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.082

99.823

0.58
  pilB Acinetobacter baylyi ADP1

57.829

99.645

0.576
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.553
  pilB Vibrio cholerae strain A1552

51.51

99.823

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

51.111

95.745

0.489
  pilB Vibrio campbellii strain DS40M4

48.759

100

0.488
  pilF Neisseria gonorrhoeae MS11

47.687

99.645

0.475
  pilF Thermus thermophilus HB27

37.809

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.686

97.163

0.376

Multiple sequence alignment