Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   H1W74_RS08475 Genome accession   NZ_LR822031
Coordinates   1643868..1644809 (-) Length   313 a.a.
NCBI ID   WP_022096896.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_1047     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1638868..1649809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W74_RS08435 (STHERMO_1926) - 1638944..1640137 (-) 1194 WP_022096898.1 acetate kinase -
  H1W74_RS08440 (STHERMO_1927) comGH 1640193..1641149 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  H1W74_RS08445 (STHERMO_1928) comGG 1641194..1641511 (-) 318 WP_002952035.1 competence type IV pilus minor pilin ComGG Machinery gene
  H1W74_RS08450 (STHERMO_1929) comGF 1641489..1641926 (-) 438 WP_022096897.1 competence type IV pilus minor pilin ComGF Machinery gene
  H1W74_RS08455 (STHERMO_1930) comGE 1641910..1642203 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE Machinery gene
  H1W74_RS08460 (STHERMO_1931) comGD 1642175..1642603 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  H1W74_RS08465 (STHERMO_1932) comGC 1642563..1642889 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  H1W74_RS08470 (STHERMO_1933) comGB 1642886..1643986 (-) 1101 WP_116920398.1 competence type IV pilus assembly protein ComGB Machinery gene
  H1W74_RS08475 (STHERMO_1934) comGA/cglA/cilD 1643868..1644809 (-) 942 WP_022096896.1 competence type IV pilus ATPase ComGA Machinery gene
  H1W74_RS08480 (STHERMO_1935) - 1644890..1645252 (-) 363 WP_011226630.1 DUF1033 family protein -
  H1W74_RS08485 (STHERMO_1936) rpoC 1645411..1649049 (-) 3639 WP_022096895.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35409.56 Da        Isoelectric Point: 6.3927

>NTDB_id=1010766 H1W74_RS08475 WP_022096896.1 1643868..1644809(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_1047]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1010766 H1W74_RS08475 WP_022096896.1 1643868..1644809(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_1047]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCTATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTCTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGTGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.595

99.361

0.652
  comGA Streptococcus mutans UA159

64.856

100

0.649
  comGA Streptococcus mutans UA140

64.856

100

0.649
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.309

99.361

0.639
  comGA Streptococcus gordonii str. Challis substr. CH1

63.259

100

0.633
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.538

99.681

0.613
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

38.816

97.125

0.377