Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ABFV67_RS01295 Genome accession   NZ_CP158116
Coordinates   283358..285079 (+) Length   573 a.a.
NCBI ID   WP_332396398.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain IS021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 278358..290079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFV67_RS01275 (ABFV67_01275) pilM 280649..281656 (+) 1008 WP_154169995.1 type IV pilus assembly protein PilM Machinery gene
  ABFV67_RS01280 (ABFV67_01280) pilN 281646..282239 (+) 594 WP_158144634.1 PilN domain-containing protein Machinery gene
  ABFV67_RS01285 (ABFV67_01285) pilO 282232..282822 (+) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABFV67_RS01290 (ABFV67_01290) pilP 282812..283333 (+) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  ABFV67_RS01295 (ABFV67_01295) pilQ 283358..285079 (+) 1722 WP_332396398.1 type IV pilus secretin PilQ family protein Machinery gene
  ABFV67_RS01300 (ABFV67_01300) aroK 285266..285784 (+) 519 WP_040903956.1 shikimate kinase AroK -
  ABFV67_RS01305 (ABFV67_01305) aroB 285813..286898 (+) 1086 WP_004394485.1 3-dehydroquinate synthase -
  ABFV67_RS01310 (ABFV67_01310) - 287042..288490 (+) 1449 WP_004394486.1 AAA family ATPase -
  ABFV67_RS01315 (ABFV67_01315) - 288559..289374 (+) 816 WP_004394487.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62160.56 Da        Isoelectric Point: 4.8607

>NTDB_id=1009810 ABFV67_RS01295 WP_332396398.1 283358..285079(+) (pilQ) [Vibrio metschnikovii strain IS021]
MIKGLPSSIAVVLRYLCITLWLGLALTTQAALASDSNQFKHIDFRVTPDKAAVIVIELASASSVVDVQKTTQGLSIELLK
TDVAEEQLYLLDVQDFATIVESVEVFRQTPSTLLLAKIHGPFHYDYQLKGRYVEVTITPLKAEVQSAPANLLEPSAKLIS
INFQDIPVRNVLQLIADYNQFNLVVSDSVLGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVILVAPKAELDLREKQQL
EKARLAEELGDLQSAIIKISFAKASDIAEMIGGTGAVNMLSERGSISIDERTNALLIRELPENIHVIREIISALDIPVKQ
VQIEARIVTVNEGNLDELGIRWGVSSTRGNNTVGGSIEGNLTGIGLYQPEDGSEMINNFLNVNLGATAANASSIAFQVAK
LGSGTLLDLELSAMQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVAFKKAVLSLQVTPQITPDNRLVL
DLSVTQDRPGDIVKTGTGEAVAIHTQRIGTQVLVNNGETVVLGGIFQHSITNTVDKVPLLGDLPLLGALFRRSYQQMGKS
ELLIFVTPKVVIQ

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1009810 ABFV67_RS01295 WP_332396398.1 283358..285079(+) (pilQ) [Vibrio metschnikovii strain IS021]
ATGATTAAAGGATTACCTTCAAGTATCGCGGTAGTGCTGCGCTATCTGTGCATCACGCTATGGCTAGGTTTAGCATTGAC
GACGCAGGCTGCTCTAGCGAGTGATAGCAATCAGTTTAAACACATCGATTTTCGGGTAACCCCCGACAAAGCCGCGGTGA
TTGTGATTGAGTTAGCGTCGGCATCGAGTGTGGTTGATGTCCAGAAAACGACGCAGGGGTTGAGTATTGAATTATTAAAA
ACCGATGTGGCTGAGGAACAGCTTTATTTACTTGATGTTCAAGATTTCGCAACCATAGTTGAGAGTGTGGAAGTTTTTCG
TCAAACACCAAGCACGTTGTTGTTGGCTAAAATACACGGTCCCTTTCATTATGATTATCAGCTCAAAGGTCGTTACGTTG
AAGTGACGATCACGCCGTTAAAAGCCGAGGTTCAAAGTGCGCCAGCCAATCTACTTGAACCATCAGCCAAGCTGATTTCG
ATTAACTTTCAAGACATTCCGGTACGTAACGTTTTACAACTGATCGCTGATTATAATCAGTTTAACTTAGTGGTATCTGA
TTCAGTGTTAGGAAATTTAACCCTGCGTTTAGATGGTGTGCCATGGCAGCAAGTGTTGGACATTATTTTGCAGGTGAAAG
GGTTGGATAAACGGGTTGAGGGCAATGTGATTTTGGTAGCGCCTAAGGCGGAACTCGATTTACGTGAAAAACAACAGCTG
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGAGACTTGCAGTCAGCCATCATCAAAATAAGTTTCGCGAAGGCGTCAGATAT
CGCTGAGATGATTGGTGGGACGGGAGCGGTGAATATGCTCTCTGAACGAGGTTCAATCAGTATCGATGAACGCACCAATG
CCTTATTAATTCGCGAATTGCCGGAAAATATTCATGTGATCCGAGAGATCATTTCGGCGTTAGATATCCCTGTTAAGCAG
GTACAAATTGAAGCGCGAATTGTGACCGTGAATGAAGGTAATCTTGATGAACTCGGTATTCGTTGGGGCGTGAGCTCAAC
ACGCGGTAATAATACAGTTGGTGGTTCAATAGAAGGTAACTTAACGGGTATTGGTCTCTATCAACCAGAAGATGGTAGCG
AAATGATCAATAACTTCCTTAATGTCAATTTGGGAGCCACAGCAGCGAATGCTTCGAGCATCGCGTTTCAGGTGGCAAAG
CTGGGTTCAGGGACACTGCTCGATCTTGAACTTTCAGCGATGCAGCGAGAATCGAAGGCGGAAATTATTTCCAGTCCACG
GCTCATCACTACCAATAAAAAGCCAGCTTATATAGAGCAGGGGACAGAGATCCCCTATTTGGAATCGTCTTCCAGCGGTG
CGACTACGGTGGCATTTAAGAAAGCGGTACTGAGTTTACAGGTTACCCCGCAGATTACGCCGGATAATCGTTTGGTGCTG
GATTTAAGTGTTACCCAAGACCGACCTGGCGATATTGTCAAAACCGGTACCGGAGAAGCGGTAGCGATCCACACGCAACG
GATTGGTACTCAAGTTTTAGTCAATAATGGCGAAACCGTGGTACTGGGCGGTATTTTCCAACACAGCATCACTAATACGG
TCGATAAAGTGCCATTACTGGGTGATTTACCGTTACTCGGTGCGCTTTTTCGACGTAGCTATCAGCAAATGGGCAAAAGT
GAGCTACTGATTTTTGTTACTCCAAAGGTTGTAATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)

Bacterial type II and III secretion system protein (Secretin)

(413-571)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.776

100

0.773
  pilQ Vibrio cholerae strain A1552

76.776

100

0.773
  pilQ Vibrio campbellii strain DS40M4

75.818

95.986

0.728
  pilQ Legionella pneumophila strain Lp02

41.852

94.241

0.394
  pilQ Legionella pneumophila strain ERS1305867

41.852

94.241

0.394
  pilQ Pseudomonas aeruginosa PAK

40.468

97.033

0.393
  comQ Acinetobacter baylyi ADP1

39.076

98.255

0.384
  pilQ Acinetobacter baumannii D1279779

36.269

100

0.366
  pilQ Acinetobacter baumannii strain A118

36.269

100

0.366

Multiple sequence alignment