Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABFV67_RS01275 Genome accession   NZ_CP158116
Coordinates   280649..281656 (+) Length   335 a.a.
NCBI ID   WP_154169995.1    Uniprot ID   -
Organism   Vibrio metschnikovii strain IS021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 275649..286656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFV67_RS01260 (ABFV67_01260) - 276551..276838 (-) 288 WP_040903944.1 DUF3624 domain-containing protein -
  ABFV67_RS01265 (ABFV67_01265) oxyR 277074..277973 (+) 900 WP_004394478.1 DNA-binding transcriptional regulator OxyR -
  ABFV67_RS01270 (ABFV67_01270) - 278027..280507 (-) 2481 WP_110167722.1 PBP1A family penicillin-binding protein -
  ABFV67_RS01275 (ABFV67_01275) pilM 280649..281656 (+) 1008 WP_154169995.1 type IV pilus assembly protein PilM Machinery gene
  ABFV67_RS01280 (ABFV67_01280) pilN 281646..282239 (+) 594 WP_158144634.1 PilN domain-containing protein Machinery gene
  ABFV67_RS01285 (ABFV67_01285) pilO 282232..282822 (+) 591 WP_154169997.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABFV67_RS01290 (ABFV67_01290) pilP 282812..283333 (+) 522 WP_040903953.1 pilus assembly protein PilP Machinery gene
  ABFV67_RS01295 (ABFV67_01295) pilQ 283358..285079 (+) 1722 WP_332396398.1 type IV pilus secretin PilQ family protein Machinery gene
  ABFV67_RS01300 (ABFV67_01300) aroK 285266..285784 (+) 519 WP_040903956.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37445.04 Da        Isoelectric Point: 7.4417

>NTDB_id=1009806 ABFV67_RS01275 WP_154169995.1 280649..281656(+) (pilM) [Vibrio metschnikovii strain IS021]
MSKSLVIGVDIGHHSIKAVALKPNGDNYSLHGCTEIVITDDIFADNDTLNYQKIVKKLKELKKSLPLFSRKVAIALPDTA
VISKVLQIDSDLDPREKEFAIYQAFSHQSPLPMEELSLDFVPLPIQAFAKNRTATYQVYATRREVVASRLSAIQQAGLEA
TLLDVQLHGLAQIWQQVSQAQQRSNWMLVDVGYTQTTLCIDFADKAPFYKDIALGTRWFELPSLAETMARSDERNQWLFN
RLFVERLTRQLQLFTSVHGQQSLAGIWLSGGGAMMDGLAETITEQLGLPCERFAPLATFVNKITQRKRLNDQPKFTTAAG
LALRGIAWLESGHVA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=1009806 ABFV67_RS01275 WP_154169995.1 280649..281656(+) (pilM) [Vibrio metschnikovii strain IS021]
ATGAGTAAATCATTAGTTATTGGTGTCGATATCGGTCATCACAGCATCAAAGCTGTGGCTCTTAAACCAAATGGCGACAA
TTATTCGCTACACGGTTGCACAGAAATCGTCATTACTGACGACATTTTCGCCGATAACGACACGTTGAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGAGTTTGCCGCTATTTAGTCGCAAGGTTGCGATTGCACTGCCTGATACGGCG
GTAATTAGTAAAGTATTACAAATAGATAGCGACTTAGACCCTCGTGAAAAAGAGTTTGCTATTTATCAGGCTTTTTCTCA
TCAATCGCCGTTGCCGATGGAAGAACTGAGCTTAGATTTTGTTCCTTTACCGATTCAAGCGTTCGCTAAAAACCGAACAG
CGACCTATCAGGTGTATGCCACCCGAAGAGAAGTCGTCGCGAGTCGATTATCGGCGATTCAACAAGCCGGACTGGAAGCA
ACCTTACTGGATGTGCAATTACATGGTCTGGCTCAGATTTGGCAGCAGGTGAGTCAGGCTCAACAGCGCTCGAATTGGAT
GTTGGTCGATGTTGGTTATACCCAAACAACGTTATGTATCGATTTTGCTGATAAAGCGCCGTTTTATAAGGATATTGCTT
TAGGTACACGCTGGTTTGAATTGCCGTCTCTGGCGGAAACGATGGCGCGCAGCGATGAACGTAACCAATGGCTATTCAAT
CGATTATTTGTCGAACGCCTAACCCGTCAACTGCAGTTATTTACTTCCGTTCATGGTCAGCAAAGCCTAGCGGGGATCTG
GTTATCTGGTGGCGGGGCGATGATGGATGGGCTAGCAGAAACCATCACCGAGCAGCTTGGATTACCCTGTGAACGGTTCG
CGCCATTAGCGACTTTTGTCAACAAAATCACTCAACGTAAGCGGTTAAATGATCAGCCTAAATTCACGACGGCTGCTGGT
CTTGCGTTGCGAGGAATAGCATGGTTGGAGAGTGGGCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(240-325)

Type IV pilus assembly protein PilM; (PilM_2)

(8-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

60.177

100

0.609
  pilM Vibrio campbellii strain DS40M4

45.97

100

0.46

Multiple sequence alignment